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- W4294051946 abstract "Even after over 2 years of the COVID-19 pandemic, research on rapid, inexpensive, and accurate tests remains essential for controlling and avoiding the global spread of SARS-CoV-2 across the planet during a potential reappearance in future global waves or regional outbreaks. Assessment of serological responses for COVID-19 can be beneficial for population-level surveillance purposes, supporting the development of novel vaccines and evaluating the efficacy of different immunization programs. This can be especially relevant for broadly used inactivated whole virus vaccines, such as CoronaVac, which produced lower titers of neutralizing antibodies. and showed lower efficacy for specific groups such as the elderly and immunocompromised. We developed an impedimetric biosensor based on the immobilization of SARS-CoV-2 recombinant trimeric spike protein (S protein) on zinc oxide nanorod (ZnONR)-modified fluorine-doped tin oxide substrates for COVID-19 serology testing. Due to electrostatic interactions, the negatively charged S protein was immobilized via physical adsorption. The electrochemical response of the immunosensor was measured at each modification step and characterized by scanning electron microscopy and electrochemical techniques. We successfully evaluated the applicability of the modified ZnONR electrodes using serum samples from COVID-19 convalescent individuals, CoronaVac-vaccinated with or without positive results for SARS-CoV-2 infection, and pre-pandemic samples from healthy volunteers as controls. ELISA for IgG anti-SARS-CoV-2 spike protein was performed for comparison, and ELISA for IgG anti-RBDs of seasonal coronavirus (HCoVs) was used to test the specificity of immunosensor detection. No cross-reactivity with HCoVs was detected using the ZnONR immunosensor, and more interestingly, the sensor presented higher sensitivity when compared to negative ELISA results. The results demonstrate that the ZnONRs/spike-modified electrode displayed sensitive results for convalescents and vaccinated samples and shows excellent potential as a tool for the population’s assessment and monitoring of seroconversion and seroprevalence." @default.
- W4294051946 created "2022-09-01" @default.
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- W4294051946 date "2022-09-01" @default.
- W4294051946 modified "2023-10-15" @default.
- W4294051946 title "Electrochemical Immunosensors Based on Zinc Oxide Nanorods for Detection of Antibodies Against SARS-CoV-2 Spike Protein in Convalescent and Vaccinated Individuals" @default.
- W4294051946 cites W1496374733 @default.
- W4294051946 cites W1582964774 @default.
- W4294051946 cites W1966027864 @default.
- W4294051946 cites W1969400004 @default.
- W4294051946 cites W1976877249 @default.
- W4294051946 cites W1979495766 @default.
- W4294051946 cites W1979797405 @default.
- W4294051946 cites W1987664387 @default.
- W4294051946 cites W1988874625 @default.
- W4294051946 cites W1997194058 @default.
- W4294051946 cites W1998996983 @default.
- W4294051946 cites W2003129296 @default.
- W4294051946 cites W2012310040 @default.
- W4294051946 cites W2015181132 @default.
- W4294051946 cites W2019603139 @default.
- W4294051946 cites W2023931848 @default.
- W4294051946 cites W2026911097 @default.
- W4294051946 cites W2032150100 @default.
- W4294051946 cites W2044447394 @default.
- W4294051946 cites W2046378459 @default.
- W4294051946 cites W2051955688 @default.
- W4294051946 cites W2055316580 @default.
- W4294051946 cites W2058095352 @default.
- W4294051946 cites W2058194180 @default.
- W4294051946 cites W2063228439 @default.
- W4294051946 cites W2068717657 @default.
- W4294051946 cites W2069542935 @default.
- W4294051946 cites W2072715410 @default.
- W4294051946 cites W2073685981 @default.
- W4294051946 cites W2081651620 @default.
- W4294051946 cites W2081842095 @default.
- W4294051946 cites W2082045674 @default.
- W4294051946 cites W2126914215 @default.
- W4294051946 cites W2136359657 @default.
- W4294051946 cites W2141918308 @default.
- W4294051946 cites W2161136893 @default.
- W4294051946 cites W2166776437 @default.
- W4294051946 cites W2170803509 @default.
- W4294051946 cites W2188414870 @default.
- W4294051946 cites W2194741285 @default.
- W4294051946 cites W2300423099 @default.
- W4294051946 cites W2321760542 @default.
- W4294051946 cites W2323446833 @default.
- W4294051946 cites W2414360444 @default.
- W4294051946 cites W2489179305 @default.
- W4294051946 cites W2522305008 @default.
- W4294051946 cites W2587581450 @default.
- W4294051946 cites W2604222709 @default.
- W4294051946 cites W2606330582 @default.
- W4294051946 cites W2614000815 @default.
- W4294051946 cites W2766085136 @default.
- W4294051946 cites W2770877241 @default.
- W4294051946 cites W2787378999 @default.
- W4294051946 cites W2793350103 @default.
- W4294051946 cites W2802872841 @default.
- W4294051946 cites W2892332176 @default.
- W4294051946 cites W2910494580 @default.
- W4294051946 cites W2912722395 @default.
- W4294051946 cites W2922060538 @default.
- W4294051946 cites W2931960508 @default.
- W4294051946 cites W2948210487 @default.
- W4294051946 cites W2970171921 @default.
- W4294051946 cites W2979958388 @default.
- W4294051946 cites W2999689790 @default.
- W4294051946 cites W3013886024 @default.
- W4294051946 cites W3021274772 @default.
- W4294051946 cites W3029021356 @default.
- W4294051946 cites W3033626723 @default.
- W4294051946 cites W3036461115 @default.
- W4294051946 cites W3046463683 @default.
- W4294051946 cites W3047416189 @default.
- W4294051946 cites W3080266293 @default.
- W4294051946 cites W3081977270 @default.
- W4294051946 cites W3090829291 @default.
- W4294051946 cites W3092246152 @default.
- W4294051946 cites W3092987038 @default.
- W4294051946 cites W3093877663 @default.
- W4294051946 cites W3094412000 @default.
- W4294051946 cites W3095205492 @default.
- W4294051946 cites W3111314177 @default.
- W4294051946 cites W3113088690 @default.
- W4294051946 cites W3113774549 @default.
- W4294051946 cites W3120305145 @default.