Matches in SemOpenAlex for { <https://semopenalex.org/work/W4294123289> ?p ?o ?g. }
- W4294123289 abstract "Cryopreserved peripheral blood mononuclear cells (PBMCs) are frequently collected and provide disease- and treatment-relevant data in clinical studies. Here, we developed combined protein (40 antibodies) and transcript single-cell (sc)RNA sequencing (scRNA-seq) in PBMCs.Among 31 participants in the Women's Interagency HIV Study (WIHS), we sequenced 41,611 cells. Using Boolean gating followed by Seurat UMAPs (tool for visualizing high-dimensional data) and Louvain clustering, we identified 50 subsets among CD4+ T, CD8+ T, B, NK cells, and monocytes. This resolution was superior to flow cytometry, mass cytometry, or scRNA-seq without antibodies. Combined protein and transcript scRNA-seq allowed for the assessment of disease-related changes in transcriptomes and cell type proportions. As a proof-of-concept, we showed such differences between healthy and matched individuals living with HIV with and without cardiovascular disease.In conclusion, combined protein and transcript scRNA sequencing is a suitable and powerful method for clinical investigations using PBMCs." @default.
- W4294123289 created "2022-09-02" @default.
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- W4294123289 date "2022-09-01" @default.
- W4294123289 modified "2023-10-14" @default.
- W4294123289 title "Combined protein and transcript single-cell RNA sequencing in human peripheral blood mononuclear cells" @default.
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