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- W4294204330 abstract "Many questions surround the underlying mechanism for the differential metabolic processing observed for the prion protein (PrP) in healthy and prion-infected mammals. Foremost, the physiological α-cleavage of PrP interrupts a region critical for both toxicity and conversion of cellular PrP (PrP C ) into its misfolded pathogenic isoform (PrP Sc ) by generating a glycosylphosphatidylinositol (GPI)-anchored C1 fragment. During prion diseases, alternative β-cleavage of PrP becomes prominent, producing a GPI-anchored C2 fragment with this particular region intact. It remains unexplored whether physical up-regulation of α-cleavage can inhibit disease progression. Furthermore, several pieces of evidence indicate that a disintegrin and metalloproteinase (ADAM) 10 and ADAM17 play a much smaller role in the α-cleavage of PrP C than originally believed, thus presenting the need to identify the primary protease(s) responsible. For this purpose, we characterized the ability of plasmin to perform PrP α-cleavage. Then, we conducted functional assays using protein misfolding cyclic amplification (PMCA) and prion-infected cell lines to clarify the role of plasmin-mediated α-cleavage during prion propagation. Here, we demonstrated an inhibitory role of plasmin for PrP Sc formation through PrP α-cleavage that increased C1 fragments resulting in reduced prion conversion compared with non-treated PMCA and cell cultures. The reduction of prion infectious titer in the bioassay of plasmin-treated PMCA material also supported the inhibitory role of plasmin on PrP Sc replication. Our results suggest that plasmin-mediated endoproteolytic cleavage of PrP may be an important event to prevent prion propagation." @default.
- W4294204330 created "2022-09-02" @default.
- W4294204330 creator A5014827285 @default.
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- W4294204330 date "2022-09-02" @default.
- W4294204330 modified "2023-10-14" @default.
- W4294204330 title "Endoproteolysis of cellular prion protein by plasmin hinders propagation of prions" @default.
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- W4294204330 cites W1998098913 @default.
- W4294204330 cites W2000036908 @default.
- W4294204330 cites W2002466165 @default.
- W4294204330 cites W2005583362 @default.
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- W4294204330 cites W2006299913 @default.
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- W4294204330 cites W2010584732 @default.
- W4294204330 cites W2011779831 @default.
- W4294204330 cites W2019748604 @default.
- W4294204330 cites W2020146455 @default.
- W4294204330 cites W2022392367 @default.
- W4294204330 cites W2023531011 @default.
- W4294204330 cites W2028373033 @default.
- W4294204330 cites W2030575994 @default.
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- W4294204330 cites W2031682941 @default.
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- W4294204330 cites W2034776372 @default.
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- W4294204330 cites W2047370333 @default.
- W4294204330 cites W2049711833 @default.
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- W4294204330 cites W2067604578 @default.
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- W4294204330 cites W2073854759 @default.
- W4294204330 cites W2075955495 @default.
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- W4294204330 cites W2087197243 @default.
- W4294204330 cites W2088094466 @default.
- W4294204330 cites W2090973003 @default.
- W4294204330 cites W2095913655 @default.
- W4294204330 cites W2103549354 @default.
- W4294204330 cites W2103700658 @default.
- W4294204330 cites W2104759293 @default.
- W4294204330 cites W2111291323 @default.
- W4294204330 cites W2121737241 @default.
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- W4294204330 cites W2141387514 @default.
- W4294204330 cites W2141570128 @default.
- W4294204330 cites W2141701425 @default.
- W4294204330 cites W2149529721 @default.
- W4294204330 cites W2163326577 @default.
- W4294204330 cites W2164672119 @default.
- W4294204330 cites W2164683872 @default.
- W4294204330 cites W2171526610 @default.
- W4294204330 cites W2217289029 @default.
- W4294204330 cites W2297182267 @default.
- W4294204330 cites W2324660492 @default.
- W4294204330 cites W2416762961 @default.
- W4294204330 cites W2561231882 @default.
- W4294204330 cites W4206843970 @default.
- W4294204330 cites W4244789804 @default.
- W4294204330 cites W4321317599 @default.
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- W4294204330 doi "https://doi.org/10.3389/fnmol.2022.990136" @default.
- W4294204330 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36117913" @default.
- W4294204330 hasPublicationYear "2022" @default.
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