Matches in SemOpenAlex for { <https://semopenalex.org/work/W4294384962> ?p ?o ?g. }
- W4294384962 abstract "Abstract Polysialic acid (polySia) is a long homopolymer consisting of α2,8-linked sialic acid with tightly regulated expression in humans. In healthy adults, it occurs on cell surface glycoproteins in neuronal, reproductive, and immune tissues; however, it is aberrantly present in many cancers and its overexpression correlates with significantly increased metastasis and poor prognosis. Prompted by the observation that the MCF-7 breast cancer cell line contains only intracellular polySia, we investigated the secretion of polySia from MCF-7 cells. PolySia was found predominantly on soluble proteins in MCF-7 conditioned media, but also on extracellular vesicles (EVs), secreted from the cells. Since MCF-7 cells do not express known polysialylated proteins, we developed a robust method for purifying polysialylated proteins that uses a metabolic labelling strategy to introduce a bioorthogonal functionality into polySia. Using this method we identified three previously unknown polysialylated proteins, and found that two of these proteins - AGR2 and QSOX2 – were secreted from MCF-7 cells. We confirmed that QSOX2 found in EV-depleted MCF-7 cell conditioned media was polysialylated. Herein we report the secretion of polysialic acid on both soluble and EV-associated proteins from MCF-7 cancer cells and introduce a new method to efficiently identify polysialylated proteins. These findings have exciting implications for understanding the roles of polySia in cancer progression and metastasis and for identifying new cancer biomarkers." @default.
- W4294384962 created "2022-09-03" @default.
- W4294384962 creator A5032091634 @default.
- W4294384962 creator A5036423260 @default.
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- W4294384962 creator A5066994524 @default.
- W4294384962 creator A5071365663 @default.
- W4294384962 creator A5082843765 @default.
- W4294384962 date "2022-09-02" @default.
- W4294384962 modified "2023-09-27" @default.
- W4294384962 title "A new strategy for identifying polysialylated proteins reveals they are secreted from cancer cells as soluble proteins and as part of extracellular vesicles" @default.
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- W4294384962 doi "https://doi.org/10.1101/2022.09.01.506237" @default.
- W4294384962 hasPublicationYear "2022" @default.