FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4294631138> ?p ?o ?g. }

• W4294631138 abstract "Effective therapeutics and vaccines for coronavirus disease 2019 (COVID-19) are currently lacking because of the mutation and immune escape of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Based on the propagation characteristics of SARS-CoV-2, rapid and accurate detection of complete virions from clinical samples and the environment is critical for assessing infection risk and containing further COVID-19 outbreaks. However, currently applicable methods cannot achieve large-scale clinical application due to factors such as the high viral load, cumbersome virus isolation steps, demanding environmental conditions, and long experimental periods. In this study, we developed an immuno molecular detection method combining capture of the viral spike glycoprotein with monoclonal antibodies and nucleic acid amplification via quantitative reverse transcription PCR to rapidly and accurately detect complete virions.After constructing a novel pseudovirus, screening for specific antibodies, and optimizing the detection parameters, the assay achieved a limit of detection of 9 × 102 transduction units/mL of viral titer with high confidence (~ 95%) and excellent stability against human serum and common virus/pseudovirus. The coefficients of variation were 1.0 ~ 2.0% for intra-assay and inter-assay analyses, respectively. Compared with reverse transcription-PCR, the immunomolecular method more accurately quantified complete virions. SARS-CoV-2/pseudovirus was more stable on plastic and paper compared with aluminum and copper in the detection of SARS-CoV-2 pseudovirus under different conditions. Complete virions were detected up to 96 h after they were applied to these surfaces (except for copper), although the titer of the virions was greatly reduced.Convenient, inexpensive, and accurate complete virus detection can be applied to many fields, including monitoring the infectivity of convalescent and post-discharge patients and assessing high-risk environments (isolation rooms, operating rooms, patient living environments, and cold chain logistics). This method can also be used to detect intact virions, including Hepatitis B and C viruses, human immunodeficiency virus, influenza, and the partial pulmonary virus, which may further improve the accuracy of diagnoses and facilitate individualized and precise treatments." @default.
• W4294631138 created "2022-09-05" @default.
• W4294631138 creator A5008911769 @default.
• W4294631138 creator A5026376739 @default.
• W4294631138 creator A5027946202 @default.
• W4294631138 creator A5029673951 @default.
• W4294631138 creator A5036085392 @default.
• W4294631138 creator A5047044100 @default.
• W4294631138 creator A5049242358 @default.
• W4294631138 creator A5051830816 @default.
• W4294631138 creator A5062077711 @default.
• W4294631138 creator A5083215036 @default.
• W4294631138 date "2022-09-05" @default.
• W4294631138 modified "2023-10-11" @default.
• W4294631138 title "Immunomolecular assay based on selective virion capture by spike antibody and viral nucleic acid amplification for detecting intact SARS-CoV-2 particles" @default.
• W4294631138 cites W1966238900 @default.
• W4294631138 cites W1984335993 @default.
• W4294631138 cites W2000380840 @default.
• W4294631138 cites W2077533125 @default.
• W4294631138 cites W2082877002 @default.
• W4294631138 cites W2106881036 @default.
• W4294631138 cites W2337915946 @default.
• W4294631138 cites W2408333651 @default.
• W4294631138 cites W2562256867 @default.
• W4294631138 cites W2565695020 @default.
• W4294631138 cites W2765995584 @default.
• W4294631138 cites W2902356452 @default.
• W4294631138 cites W2990923584 @default.
• W4294631138 cites W2997926335 @default.
• W4294631138 cites W3001195213 @default.
• W4294631138 cites W3001465255 @default.
• W4294631138 cites W3002108456 @default.
• W4294631138 cites W3002539152 @default.
• W4294631138 cites W3004318991 @default.
• W4294631138 cites W3004906315 @default.
• W4294631138 cites W3008443627 @default.
• W4294631138 cites W3009912996 @default.
• W4294631138 cites W3012099172 @default.
• W4294631138 cites W3013490518 @default.
• W4294631138 cites W3013800395 @default.
• W4294631138 cites W3015636815 @default.
• W4294631138 cites W3017334101 @default.
• W4294631138 cites W3023465669 @default.
• W4294631138 cites W3030968929 @default.
• W4294631138 cites W3031297371 @default.
• W4294631138 cites W3041319886 @default.
• W4294631138 cites W3047593146 @default.
• W4294631138 cites W3048573533 @default.
• W4294631138 cites W3049713430 @default.
• W4294631138 cites W3084605929 @default.
• W4294631138 cites W3092136311 @default.
• W4294631138 cites W3094412000 @default.
• W4294631138 cites W3101317276 @default.
• W4294631138 cites W3103021984 @default.
• W4294631138 cites W3106080072 @default.
• W4294631138 cites W3110571029 @default.
• W4294631138 cites W3110630166 @default.
• W4294631138 cites W3110932919 @default.
• W4294631138 cites W3111754831 @default.
• W4294631138 cites W3125800928 @default.
• W4294631138 cites W3127798546 @default.
• W4294631138 cites W3131287340 @default.
• W4294631138 cites W3136105419 @default.
• W4294631138 cites W3137469790 @default.
• W4294631138 cites W3148220846 @default.
• W4294631138 cites W3152577942 @default.
• W4294631138 cites W3159064358 @default.
• W4294631138 cites W3165205308 @default.
• W4294631138 cites W3171186796 @default.
• W4294631138 cites W3185425927 @default.
• W4294631138 cites W3187192250 @default.
• W4294631138 cites W3202168443 @default.
• W4294631138 cites W3204888995 @default.
• W4294631138 cites W4248668370 @default.
• W4294631138 doi "https://doi.org/10.1186/s12951-022-01558-8" @default.
• W4294631138 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36064407" @default.
• W4294631138 hasPublicationYear "2022" @default.
• W4294631138 type Work @default.
• W4294631138 citedByCount "0" @default.
• W4294631138 crossrefType "journal-article" @default.
• W4294631138 hasAuthorship W4294631138A5008911769 @default.
• W4294631138 hasAuthorship W4294631138A5026376739 @default.
• W4294631138 hasAuthorship W4294631138A5027946202 @default.
• W4294631138 hasAuthorship W4294631138A5029673951 @default.
• W4294631138 hasAuthorship W4294631138A5036085392 @default.
• W4294631138 hasAuthorship W4294631138A5047044100 @default.
• W4294631138 hasAuthorship W4294631138A5049242358 @default.
• W4294631138 hasAuthorship W4294631138A5051830816 @default.
• W4294631138 hasAuthorship W4294631138A5062077711 @default.
• W4294631138 hasAuthorship W4294631138A5083215036 @default.
• W4294631138 hasBestOaLocation W42946311381 @default.
• W4294631138 hasConcept C104317684 @default.
• W4294631138 hasConcept C119128265 @default.
• W4294631138 hasConcept C142724271 @default.
• W4294631138 hasConcept C153911025 @default.
• W4294631138 hasConcept C156719811 @default.
• W4294631138 hasConcept C159047783 @default.
• W4294631138 hasConcept C159654299 @default.
• W4294631138 hasConcept C185592680 @default.
• W4294631138 hasConcept C203014093 @default.

• next