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- W4294647046 abstract "MicroRNA-21 (miRNA-21) is a common biomarker with high expression in breast tumors. Therefore, sensitive detection of miRNA-21 is of great significance for clinical breast tumor diagnosis. A TH/rGO/CMK-3/AuNPs nanocomposite is composed of thionine (TH), reduced graphene oxide (rGO), ordered mesoporous carbon (CMK-3), and gold nanoparticles (AuNPs), which help to increase the specific surface area of a glassy carbon electrode (GCE) and to amplify the DPV signal. Meanwhile, methylene blue (MB) was combined with the capture probe guanine and absorbed by the composite material to mediate the differential pulse voltammetry (DPV) of the obtained miRNA biosensor. The current response decreased with increasing miRNA-21 concentration under optimal conditions. The biosensor responds to miRNA-21 in the 0.1fM-1 pM concentration range, and the detection limit (LOD) was 0.046 fM. Moreover, human serum samples were effectively detected utilizing the miRNA-21 biosensor with satisfactory results." @default.
- W4294647046 created "2022-09-05" @default.
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- W4294647046 date "2022-12-01" @default.
- W4294647046 modified "2023-10-15" @default.
- W4294647046 title "A novel conductive nanocomposite-based biosensor for ultrasensitive detection of microRNA-21 in serum, using methylene blue as mediator" @default.
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- W4294647046 doi "https://doi.org/10.1016/j.bioelechem.2022.108256" @default.
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