FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4294662011> ?p ?o ?g. }

• W4294662011 abstract "Abstract Pests and pathogens are a continuous threat to the health of Western honey bees Apis mellifera L. Monitoring honey bee colonies for arthropod pests, disease-causing bacteria and fungi, and early detection of new invasions is essential to maintain the pollination services provided by honey bees. Here, we investigated the feasibility of using eDNA metabarcoding to detect honey bee pests and pathogens in their hives and across their foraging environment. We sampled 13 different surfaces within and outside hives from our test apiary to determine where the most informative eDNA could be obtained. Furthermore, we compared two main eDNA collection techniques – wiping surfaces with moistened forensic swabs and using a spray/wash technique that washed surface DNA into a bucket, before collecting the DNA on a filter. We used DNA metabarcoding with universal primer sets to target arthropod, bacterial and fungal communities. Our results showed that most surfaces yielded usable DNA, and that results of the swabs and spray/wash methods were similar when they could be applied to the same surface. We were able to detect DNA from the small hive beetle ( Aethina tumida), Varroa destructor mites and European foulbrood ( Melissococcus plutonius ), all of which matched our visual observations of clinical signs of these pests and pathogens in the hives we tested. DNA from some species was location specific, which has implications for using eDNA as a monitoring tool. Collectively, our data show that eDNA metabarcoding can accurately detect DNA from arthropods and microbes honey bees contact and has the potential to provide information on disease and pest incidence, Apis species identity, and A. mellifera subspecies identity of the colony and/or apiary. In sum, eDNA metabarcoding can be used as a comprehensive molecular predictor tool for colony health surveys." @default.
• W4294662011 created "2022-09-06" @default.
• W4294662011 creator A5002502992 @default.
• W4294662011 creator A5002743749 @default.
• W4294662011 creator A5030080461 @default.
• W4294662011 creator A5048754462 @default.
• W4294662011 creator A5081859423 @default.
• W4294662011 creator A5087885045 @default.
• W4294662011 date "2022-09-02" @default.
• W4294662011 modified "2023-09-24" @default.
• W4294662011 title "Novel eDNA approaches to monitor Western honey bee (<i>Apis mellifera</i>) microbial and arthropod communities" @default.
• W4294662011 cites W1515010604 @default.
• W4294662011 cites W1583249335 @default.
• W4294662011 cites W1592352451 @default.
• W4294662011 cites W1777567968 @default.
• W4294662011 cites W1998476131 @default.
• W4294662011 cites W2000682768 @default.
• W4294662011 cites W2001304828 @default.
• W4294662011 cites W2002504652 @default.
• W4294662011 cites W2027936653 @default.
• W4294662011 cites W2029250845 @default.
• W4294662011 cites W2036897871 @default.
• W4294662011 cites W2044572367 @default.
• W4294662011 cites W2054908644 @default.
• W4294662011 cites W2055817596 @default.
• W4294662011 cites W2056279562 @default.
• W4294662011 cites W2059796969 @default.
• W4294662011 cites W2059872940 @default.
• W4294662011 cites W2064518489 @default.
• W4294662011 cites W2066598948 @default.
• W4294662011 cites W2074583233 @default.
• W4294662011 cites W2090660388 @default.
• W4294662011 cites W2098035523 @default.
• W4294662011 cites W2099546198 @default.
• W4294662011 cites W2104121186 @default.
• W4294662011 cites W2115943082 @default.
• W4294662011 cites W2121199768 @default.
• W4294662011 cites W2132291178 @default.
• W4294662011 cites W2137022411 @default.
• W4294662011 cites W2138832709 @default.
• W4294662011 cites W2140682278 @default.
• W4294662011 cites W2141624395 @default.
• W4294662011 cites W2153691236 @default.
• W4294662011 cites W2155423879 @default.
• W4294662011 cites W2158714788 @default.
• W4294662011 cites W2170027074 @default.
• W4294662011 cites W2179733967 @default.
• W4294662011 cites W2337608830 @default.
• W4294662011 cites W2401404581 @default.
• W4294662011 cites W2467179186 @default.
• W4294662011 cites W2506843120 @default.
• W4294662011 cites W2547006228 @default.
• W4294662011 cites W2616338442 @default.
• W4294662011 cites W2724127573 @default.
• W4294662011 cites W2733513970 @default.
• W4294662011 cites W2737148894 @default.
• W4294662011 cites W2791004613 @default.
• W4294662011 cites W2794904294 @default.
• W4294662011 cites W2801694332 @default.
• W4294662011 cites W2837409195 @default.
• W4294662011 cites W2897079919 @default.
• W4294662011 cites W2910071209 @default.
• W4294662011 cites W2910667598 @default.
• W4294662011 cites W2912959159 @default.
• W4294662011 cites W2913641542 @default.
• W4294662011 cites W2915013299 @default.
• W4294662011 cites W2962814267 @default.
• W4294662011 cites W2964935422 @default.
• W4294662011 cites W3006873678 @default.
• W4294662011 cites W3007995941 @default.
• W4294662011 cites W3013711054 @default.
• W4294662011 cites W3016113032 @default.
• W4294662011 cites W3016295937 @default.
• W4294662011 cites W3033457396 @default.
• W4294662011 cites W3049198040 @default.
• W4294662011 cites W3080852711 @default.
• W4294662011 cites W3093479100 @default.
• W4294662011 cites W3134405499 @default.
• W4294662011 cites W3138109540 @default.
• W4294662011 cites W3158177500 @default.
• W4294662011 cites W3166248452 @default.
• W4294662011 cites W3178009721 @default.
• W4294662011 cites W3185461384 @default.
• W4294662011 cites W3187877246 @default.
• W4294662011 cites W3212193450 @default.
• W4294662011 cites W4225007016 @default.
• W4294662011 cites W4225009814 @default.
• W4294662011 cites W4229441870 @default.
• W4294662011 cites W4250963355 @default.
• W4294662011 doi "https://doi.org/10.1101/2022.08.31.506105" @default.
• W4294662011 hasPublicationYear "2022" @default.
• W4294662011 type Work @default.
• W4294662011 citedByCount "0" @default.
• W4294662011 crossrefType "posted-content" @default.
• W4294662011 hasAuthorship W4294662011A5002502992 @default.
• W4294662011 hasAuthorship W4294662011A5002743749 @default.
• W4294662011 hasAuthorship W4294662011A5030080461 @default.
• W4294662011 hasAuthorship W4294662011A5048754462 @default.
• W4294662011 hasAuthorship W4294662011A5081859423 @default.
• W4294662011 hasAuthorship W4294662011A5087885045 @default.

• next