Matches in SemOpenAlex for { <https://semopenalex.org/work/W4294877908> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W4294877908 endingPage "536" @default.
- W4294877908 startingPage "528" @default.
- W4294877908 abstract "Alpha-ketoglutaric acid (α-KG) is a crucial intermediate with broad application prospects. However, the downstream separation of α-KG from fermentation broth with low concentration and complex components has become the bottleneck of large-scale production. In this study, α-KG was separated from the actual fermentation broth by aqueous two-phase system (ATPS) and crystallization. The optimal ATPS composed of acetone/(NH4)2SO4 was selected by studying the phase diagram and α-KG distribution behavior in different organic solvents and salts/sugars. Under the optimized conditions, the purity of α-KG products from crude fermentation broth reached 98.08%, and the one-pass extraction efficiency was 90.52%. The removal of cell debris and soluble proteins increased with the increase of acetone and (NH4)2SO4 concentrations, with the removal ratios reaching up to 99.44% and 86.54%. In addition, 92.56% of the glucose was distributed in the aqueous phase. Although the α-KG fermentation broth is more complex than the whole-cell biotransformation, impurities in the crude fermentation broth could be efficiently removed and a higher α-KG distribution coefficient was obtained. Meanwhile, the recovered (NH4)2SO4 could be reused for fermentation medium. This strategy provides an economical and effective method combined with upstream biosynthesis to recover α-KG and other bioproducts from crude fermentation broth." @default.
- W4294877908 created "2022-09-07" @default.
- W4294877908 creator A5019560977 @default.
- W4294877908 creator A5028178244 @default.
- W4294877908 creator A5056976054 @default.
- W4294877908 creator A5058691896 @default.
- W4294877908 creator A5065711224 @default.
- W4294877908 creator A5076197416 @default.
- W4294877908 creator A5089718840 @default.
- W4294877908 date "2022-11-01" @default.
- W4294877908 modified "2023-10-03" @default.
- W4294877908 title "Aqueous two-phase extraction to separate alpha-ketoglutaric acid from fermentation broth by combining crystallization" @default.
- W4294877908 cites W1166159342 @default.
- W4294877908 cites W1997480916 @default.
- W4294877908 cites W2010598280 @default.
- W4294877908 cites W2016397383 @default.
- W4294877908 cites W2019720423 @default.
- W4294877908 cites W2025513201 @default.
- W4294877908 cites W2056174775 @default.
- W4294877908 cites W2079819664 @default.
- W4294877908 cites W2083705387 @default.
- W4294877908 cites W2343993932 @default.
- W4294877908 cites W2463493657 @default.
- W4294877908 cites W2515696368 @default.
- W4294877908 cites W2556232537 @default.
- W4294877908 cites W2589796998 @default.
- W4294877908 cites W2590679997 @default.
- W4294877908 cites W2609680526 @default.
- W4294877908 cites W2734756303 @default.
- W4294877908 cites W2734983615 @default.
- W4294877908 cites W2796828096 @default.
- W4294877908 cites W2889796928 @default.
- W4294877908 cites W2891783374 @default.
- W4294877908 cites W2895628655 @default.
- W4294877908 cites W2907662122 @default.
- W4294877908 cites W2969707828 @default.
- W4294877908 cites W3004235911 @default.
- W4294877908 cites W3010062017 @default.
- W4294877908 cites W3111969163 @default.
- W4294877908 cites W3128223384 @default.
- W4294877908 cites W3153066015 @default.
- W4294877908 cites W3201724803 @default.
- W4294877908 cites W4200290325 @default.
- W4294877908 cites W4213232463 @default.
- W4294877908 cites W4293247451 @default.
- W4294877908 doi "https://doi.org/10.1016/j.jiec.2022.08.040" @default.
- W4294877908 hasPublicationYear "2022" @default.
- W4294877908 type Work @default.
- W4294877908 citedByCount "1" @default.
- W4294877908 crossrefType "journal-article" @default.
- W4294877908 hasAuthorship W4294877908A5019560977 @default.
- W4294877908 hasAuthorship W4294877908A5028178244 @default.
- W4294877908 hasAuthorship W4294877908A5056976054 @default.
- W4294877908 hasAuthorship W4294877908A5058691896 @default.
- W4294877908 hasAuthorship W4294877908A5065711224 @default.
- W4294877908 hasAuthorship W4294877908A5076197416 @default.
- W4294877908 hasAuthorship W4294877908A5089718840 @default.
- W4294877908 hasConcept C100544194 @default.
- W4294877908 hasConcept C149556010 @default.
- W4294877908 hasConcept C178790620 @default.
- W4294877908 hasConcept C184651966 @default.
- W4294877908 hasConcept C185592680 @default.
- W4294877908 hasConcept C192552737 @default.
- W4294877908 hasConcept C2781213060 @default.
- W4294877908 hasConcept C31903555 @default.
- W4294877908 hasConcept C43617362 @default.
- W4294877908 hasConcept C4725764 @default.
- W4294877908 hasConcept C55493867 @default.
- W4294877908 hasConcept C67568554 @default.
- W4294877908 hasConceptScore W4294877908C100544194 @default.
- W4294877908 hasConceptScore W4294877908C149556010 @default.
- W4294877908 hasConceptScore W4294877908C178790620 @default.
- W4294877908 hasConceptScore W4294877908C184651966 @default.
- W4294877908 hasConceptScore W4294877908C185592680 @default.
- W4294877908 hasConceptScore W4294877908C192552737 @default.
- W4294877908 hasConceptScore W4294877908C2781213060 @default.
- W4294877908 hasConceptScore W4294877908C31903555 @default.
- W4294877908 hasConceptScore W4294877908C43617362 @default.
- W4294877908 hasConceptScore W4294877908C4725764 @default.
- W4294877908 hasConceptScore W4294877908C55493867 @default.
- W4294877908 hasConceptScore W4294877908C67568554 @default.
- W4294877908 hasLocation W42948779081 @default.
- W4294877908 hasOpenAccess W4294877908 @default.
- W4294877908 hasPrimaryLocation W42948779081 @default.
- W4294877908 hasRelatedWork W1550243347 @default.
- W4294877908 hasRelatedWork W1994544842 @default.
- W4294877908 hasRelatedWork W2017363364 @default.
- W4294877908 hasRelatedWork W2355084195 @default.
- W4294877908 hasRelatedWork W2361464784 @default.
- W4294877908 hasRelatedWork W2365417736 @default.
- W4294877908 hasRelatedWork W2391525693 @default.
- W4294877908 hasRelatedWork W2392630185 @default.
- W4294877908 hasRelatedWork W2981981514 @default.
- W4294877908 hasRelatedWork W3153458004 @default.
- W4294877908 hasVolume "115" @default.
- W4294877908 isParatext "false" @default.
- W4294877908 isRetracted "false" @default.
- W4294877908 workType "article" @default.