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- W4294891990 abstract "Abstract Transgene expression in C. elegans is an important tool for understanding regulatory elements ( e.g. , promoters and enhancers), for controlling cells using optogenetic effector proteins, and for editing the (epi)genome. Insertion of single-copy transgenes into well-defined safe-harbor locations can be used when consistent expression is required ( e.g. , to compare expression from different transgenes) or when expression is required in germ cells. Here, we describe a detailed protocol for inserting single-copy transgenes using Modular Safe-harbor Transgene Insertion (MosTI) and constitutive Cas9 expression from a co-injected plasmid." @default.
- W4294891990 created "2022-09-07" @default.
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- W4294891990 date "2022-09-07" @default.
- W4294891990 modified "2023-10-16" @default.
- W4294891990 title "MosTI single-copy transgene insertion in C. elegans using constitutive Cas9 expression" @default.
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- W4294891990 doi "https://doi.org/10.21203/rs.3.rs-2006553/v2" @default.
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