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- W4294916321 abstract "We aim to familiarize the application status of metagenomic sequencing in diagnosing pulmonary infections, to compare metagenomic sequencing with traditional diagnostic methods, to conclude the advantages and limitations of metagenomic sequencing, and to provide some advice for clinical practice and some inspiration for associated researches.The data were obtained from peer-reviewed literature, white papers, and meeting reports.This review focused on the applications of untargeted metagenomic sequencing in lungs infected by bacteria, viruses, fungi, chlamydia pneumoniae, Mycoplasma pneumoniae, parasites, and other pathogens. Compared with conventional diagnostic methods, metagenomic sequencing is better in detecting novel, rare, and unexpected pathogens and being applied in co-infections. Meanwhile, it can also provide more comprehensive information about pathogens. However, metagenomic sequencing still has limitations. Also, the situations that should be applied in and how the results should be interpreted are discussed in this review.Metagenomic sequencing improves efficiency to identify pathogens compared with traditional diagnostic methods and can be applied in clinical diagnosis. However, the technology of metagenomic sequencing still needs to be improved. Also, clinicians should learn more about when to use metagenomic sequencing and how to interpret its results." @default.
- W4294916321 created "2022-09-08" @default.
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- W4294916321 date "2022-09-06" @default.
- W4294916321 modified "2023-10-15" @default.
- W4294916321 title "Advantages and challenges of metagenomic sequencing for the diagnosis of pulmonary infectious diseases" @default.
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- W4294916321 doi "https://doi.org/10.1111/crj.13538" @default.
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