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- W4294925963 abstract "SUMMARY Chloroplasts possess a considerably reduced genome that is decoded via an almost minimal set of tRNAs. These features make an excellent platform for gaining insights into fundamental mechanisms that govern protein expression. Here, we present a comprehensive and revised perspective of the mechanisms that drive codon selection in the chloroplast of Chlamydomonas reinhardtii and the functional consequences for protein expression. In order to extract this information, we applied several codon usage descriptors to genes with different expression levels. We show that highly expressed genes strongly favor translationally optimal codons, while genes with lower functional importance are rather affected by directional mutational bias. We demonstrate that codon optimality can be deduced from codon–anticodon pairing affinity and, for a small number of amino acids (leucine, arginine, serine, and isoleucine), tRNA concentrations. Finally, we review, analyze, and expand on the impact of codon usage on protein yield, secondary structures of mRNA, translation initiation and termination, and amino acid composition of proteins, as well as cotranslational protein folding. The comprehensive analysis of codon choice provides crucial insights into heterologous gene expression in the chloroplast of C. reinhardtii , which may also be applicable to other chloroplast‐containing organisms and bacteria." @default.
- W4294925963 created "2022-09-08" @default.
- W4294925963 creator A5023047887 @default.
- W4294925963 creator A5052748295 @default.
- W4294925963 creator A5053421162 @default.
- W4294925963 date "2022-10-19" @default.
- W4294925963 modified "2023-09-26" @default.
- W4294925963 title "Mechanisms governing codon usage bias and the implications for protein expression in the chloroplast of <i>Chlamydomonas reinhardtii</i>" @default.
- W4294925963 cites W1494671507 @default.
- W4294925963 cites W1504552716 @default.
- W4294925963 cites W1527267184 @default.
- W4294925963 cites W1530712791 @default.
- W4294925963 cites W1530982307 @default.
- W4294925963 cites W1550695177 @default.
- W4294925963 cites W1555159335 @default.
- W4294925963 cites W1578482749 @default.
- W4294925963 cites W1588840456 @default.
- W4294925963 cites W1589192533 @default.
- W4294925963 cites W1603411458 @default.
- W4294925963 cites W1607969426 @default.
- W4294925963 cites W1671377823 @default.
- W4294925963 cites W1919707350 @default.
- W4294925963 cites W1945878615 @default.
- W4294925963 cites W1966778471 @default.
- W4294925963 cites W1967819652 @default.
- W4294925963 cites W1968397216 @default.
- W4294925963 cites W1969633341 @default.
- W4294925963 cites W1971453673 @default.
- W4294925963 cites W1973147750 @default.
- W4294925963 cites W1973538078 @default.
- W4294925963 cites W1974072721 @default.
- W4294925963 cites W1974333371 @default.
- W4294925963 cites W1975586269 @default.
- W4294925963 cites W1977566832 @default.
- W4294925963 cites W1978561489 @default.
- W4294925963 cites W1982160717 @default.
- W4294925963 cites W1982411237 @default.
- W4294925963 cites W1982484796 @default.
- W4294925963 cites W1983106607 @default.
- W4294925963 cites W1985020066 @default.
- W4294925963 cites W1985037876 @default.
- W4294925963 cites W1986720579 @default.
- W4294925963 cites W1991135133 @default.
- W4294925963 cites W1993358499 @default.
- W4294925963 cites W1997809760 @default.
- W4294925963 cites W1999177695 @default.
- W4294925963 cites W2004674004 @default.
- W4294925963 cites W2008465577 @default.
- W4294925963 cites W2011503994 @default.
- W4294925963 cites W2011739907 @default.
- W4294925963 cites W2015365987 @default.
- W4294925963 cites W2017411951 @default.
- W4294925963 cites W2017528171 @default.
- W4294925963 cites W2018172900 @default.
- W4294925963 cites W2018590530 @default.
- W4294925963 cites W2019661196 @default.
- W4294925963 cites W2020320704 @default.
- W4294925963 cites W2022726657 @default.
- W4294925963 cites W2025569664 @default.
- W4294925963 cites W2026070637 @default.
- W4294925963 cites W2026814176 @default.
- W4294925963 cites W2029132648 @default.
- W4294925963 cites W2030564603 @default.
- W4294925963 cites W2033187951 @default.
- W4294925963 cites W2033662167 @default.
- W4294925963 cites W2034336251 @default.
- W4294925963 cites W2035215080 @default.
- W4294925963 cites W2035475950 @default.
- W4294925963 cites W2035970801 @default.
- W4294925963 cites W2036588330 @default.
- W4294925963 cites W2038379310 @default.
- W4294925963 cites W2043592686 @default.
- W4294925963 cites W2044573154 @default.
- W4294925963 cites W2046872786 @default.
- W4294925963 cites W2046975961 @default.
- W4294925963 cites W2047405192 @default.
- W4294925963 cites W2047515039 @default.
- W4294925963 cites W2048469000 @default.
- W4294925963 cites W2052413864 @default.
- W4294925963 cites W2057074984 @default.
- W4294925963 cites W2058212562 @default.
- W4294925963 cites W2058259339 @default.
- W4294925963 cites W2058315563 @default.
- W4294925963 cites W2058323291 @default.
- W4294925963 cites W2060421156 @default.
- W4294925963 cites W2062418478 @default.
- W4294925963 cites W2062678866 @default.
- W4294925963 cites W2063504601 @default.
- W4294925963 cites W2063619185 @default.
- W4294925963 cites W2063779182 @default.
- W4294925963 cites W2063933849 @default.
- W4294925963 cites W2064251716 @default.
- W4294925963 cites W2065049493 @default.
- W4294925963 cites W2065120930 @default.
- W4294925963 cites W2065371606 @default.
- W4294925963 cites W2067447790 @default.
- W4294925963 cites W2068694787 @default.
- W4294925963 cites W2070163065 @default.