Matches in SemOpenAlex for { <https://semopenalex.org/work/W4294940> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W4294940 endingPage "315" @default.
- W4294940 startingPage "307" @default.
- W4294940 abstract "Ca 2+ transients have traditionally been measured using synthetic fluorescent chelators or recombinant aequorin. Synthetic molecules provide a bright fluorescent signal, but these dyes are not easy to load and gradually leak out of cells at physiological temperatures. The use of cameleon indicators is gradually becoming more common within the Ca 2+ signaling community and the literature is rich with examples of various applications. A successful construction of GFP-fused protein indicators is facilitated greatly by careful inspection of available three-dimensional structure information of the protein or protein domain used for sensing functions. Enhanced cyan fluorescent protein (ECFP) and enhanced yellow fluorescent protein (EYFP) expression constructus. Expression vectors for many GFP family members are available through Clontech. Cameleon constructs can benefit from truncation of the last 11 C-terminal amino acids of ECFP (the minimal region to form GFP) to reduce the relative tumbling of the fluorophores. GFP family proteins have been observed to form obligate dimers and may thereby generate false-positive FRET signals." @default.
- W4294940 created "2016-06-24" @default.
- W4294940 creator A5010844468 @default.
- W4294940 creator A5016424930 @default.
- W4294940 creator A5024058200 @default.
- W4294940 date "2006-01-01" @default.
- W4294940 modified "2023-10-17" @default.
- W4294940 title "Ca2+ as a Second MessengerNew Reporters for Calcium (Cameleons and Camgaroos)" @default.
- W4294940 cites W1542263802 @default.
- W4294940 cites W1557879301 @default.
- W4294940 cites W1576575094 @default.
- W4294940 cites W1969387286 @default.
- W4294940 cites W1973828083 @default.
- W4294940 cites W1976136773 @default.
- W4294940 cites W1996160970 @default.
- W4294940 cites W1996744375 @default.
- W4294940 cites W1997192016 @default.
- W4294940 cites W2013151879 @default.
- W4294940 cites W2013412105 @default.
- W4294940 cites W2032488764 @default.
- W4294940 cites W2037668423 @default.
- W4294940 cites W2039210066 @default.
- W4294940 cites W2041194353 @default.
- W4294940 cites W2057522454 @default.
- W4294940 cites W2070164216 @default.
- W4294940 cites W2082569007 @default.
- W4294940 cites W2090212447 @default.
- W4294940 cites W2097651960 @default.
- W4294940 cites W2107913200 @default.
- W4294940 cites W2136396782 @default.
- W4294940 cites W2141260882 @default.
- W4294940 cites W2144454171 @default.
- W4294940 cites W2157199084 @default.
- W4294940 cites W66362777 @default.
- W4294940 cites W97375708 @default.
- W4294940 cites W2799449195 @default.
- W4294940 doi "https://doi.org/10.1016/b978-012164730-8/50112-x" @default.
- W4294940 hasPublicationYear "2006" @default.
- W4294940 type Work @default.
- W4294940 sameAs 4294940 @default.
- W4294940 citedByCount "0" @default.
- W4294940 crossrefType "book-chapter" @default.
- W4294940 hasAuthorship W4294940A5010844468 @default.
- W4294940 hasAuthorship W4294940A5016424930 @default.
- W4294940 hasAuthorship W4294940A5024058200 @default.
- W4294940 hasConcept C104317684 @default.
- W4294940 hasConcept C121332964 @default.
- W4294940 hasConcept C12554922 @default.
- W4294940 hasConcept C142613039 @default.
- W4294940 hasConcept C185592680 @default.
- W4294940 hasConcept C2778067846 @default.
- W4294940 hasConcept C3019336641 @default.
- W4294940 hasConcept C55493867 @default.
- W4294940 hasConcept C62520636 @default.
- W4294940 hasConcept C79879829 @default.
- W4294940 hasConcept C80933277 @default.
- W4294940 hasConcept C86803240 @default.
- W4294940 hasConcept C91881484 @default.
- W4294940 hasConcept C95444343 @default.
- W4294940 hasConcept C96305047 @default.
- W4294940 hasConceptScore W4294940C104317684 @default.
- W4294940 hasConceptScore W4294940C121332964 @default.
- W4294940 hasConceptScore W4294940C12554922 @default.
- W4294940 hasConceptScore W4294940C142613039 @default.
- W4294940 hasConceptScore W4294940C185592680 @default.
- W4294940 hasConceptScore W4294940C2778067846 @default.
- W4294940 hasConceptScore W4294940C3019336641 @default.
- W4294940 hasConceptScore W4294940C55493867 @default.
- W4294940 hasConceptScore W4294940C62520636 @default.
- W4294940 hasConceptScore W4294940C79879829 @default.
- W4294940 hasConceptScore W4294940C80933277 @default.
- W4294940 hasConceptScore W4294940C86803240 @default.
- W4294940 hasConceptScore W4294940C91881484 @default.
- W4294940 hasConceptScore W4294940C95444343 @default.
- W4294940 hasConceptScore W4294940C96305047 @default.
- W4294940 hasLocation W42949401 @default.
- W4294940 hasOpenAccess W4294940 @default.
- W4294940 hasPrimaryLocation W42949401 @default.
- W4294940 hasRelatedWork W1975629253 @default.
- W4294940 hasRelatedWork W1980726951 @default.
- W4294940 hasRelatedWork W2013484632 @default.
- W4294940 hasRelatedWork W2021692017 @default.
- W4294940 hasRelatedWork W2077482001 @default.
- W4294940 hasRelatedWork W2750805269 @default.
- W4294940 hasRelatedWork W2946216085 @default.
- W4294940 hasRelatedWork W3157204075 @default.
- W4294940 hasRelatedWork W4294940 @default.
- W4294940 hasRelatedWork W47411888 @default.
- W4294940 isParatext "false" @default.
- W4294940 isRetracted "false" @default.
- W4294940 magId "4294940" @default.
- W4294940 workType "book-chapter" @default.