Matches in SemOpenAlex for { <https://semopenalex.org/work/W4294991475> ?p ?o ?g. }
- W4294991475 abstract "In cystic fibrosis (CF), the loss of cystic fibrosis transmembrane conductance regulator (CFTR) mediated Cl− and HCO3− secretion across the epithelium acidifies the airway surface liquid (ASL). Acidic ASL alters two key host defense mechanisms: Rapid ASL bacterial killing and mucociliary transport (MCT). Aerosolized tromethamine (Tham) increases ASL pH and restores the ability of ASL to rapidly kill bacteria in CF pigs. In CF pigs, clearance of insufflated microdisks is interrupted due to abnormal mucus causing microdisks to abruptly recoil. Aerosolizing a reducing agent to break disulfide bonds that link mucins improves MCT. Here, we are interested in restoring MCT in CF by aerosolizing Tham, a buffer with a pH of 8.4. Because Tham is hypertonic to serum, we use an acidified formulation as a control. We measure MCT by tracking the caudal movement of individual tantalum microdisks with serial chest computed tomography scans. Alkaline Tham improves microdisk clearance to within the range of that seen in non-CF pigs. It also partially reverses MCT defects, including reduced microdisk recoil and elapse time until they start moving after methacholine stimulation in CF pig airways. The effect is not due to hypertonicity, as it is not seen with acidified Tham or hypertonic saline. This finding indicates acidic ASL impairs CF MCT and suggests that alkalinization of ASL pH with inhaled Tham may improve CF airway disease." @default.
- W4294991475 created "2022-09-09" @default.
- W4294991475 creator A5003544851 @default.
- W4294991475 creator A5022422924 @default.
- W4294991475 creator A5023131199 @default.
- W4294991475 creator A5036535003 @default.
- W4294991475 creator A5046834672 @default.
- W4294991475 creator A5057269820 @default.
- W4294991475 creator A5076021105 @default.
- W4294991475 date "2022-09-01" @default.
- W4294991475 modified "2023-10-15" @default.
- W4294991475 title "Tromethamine improves mucociliary clearance in cystic fibrosis pigs" @default.
- W4294991475 cites W1571437601 @default.
- W4294991475 cites W1594696872 @default.
- W4294991475 cites W1775439135 @default.
- W4294991475 cites W1845641771 @default.
- W4294991475 cites W1968152866 @default.
- W4294991475 cites W1990179332 @default.
- W4294991475 cites W1990301853 @default.
- W4294991475 cites W1997180527 @default.
- W4294991475 cites W1997182007 @default.
- W4294991475 cites W2000313413 @default.
- W4294991475 cites W2007770546 @default.
- W4294991475 cites W2016417321 @default.
- W4294991475 cites W2019385365 @default.
- W4294991475 cites W2022941489 @default.
- W4294991475 cites W2024990146 @default.
- W4294991475 cites W2038865117 @default.
- W4294991475 cites W2039462910 @default.
- W4294991475 cites W2044380511 @default.
- W4294991475 cites W2046370810 @default.
- W4294991475 cites W2052657993 @default.
- W4294991475 cites W2054716883 @default.
- W4294991475 cites W2061186101 @default.
- W4294991475 cites W2069252849 @default.
- W4294991475 cites W2080479975 @default.
- W4294991475 cites W2089214886 @default.
- W4294991475 cites W2090937593 @default.
- W4294991475 cites W2093757202 @default.
- W4294991475 cites W2099896698 @default.
- W4294991475 cites W2103043501 @default.
- W4294991475 cites W2108676410 @default.
- W4294991475 cites W2113830910 @default.
- W4294991475 cites W2120911787 @default.
- W4294991475 cites W2121159616 @default.
- W4294991475 cites W2122517176 @default.
- W4294991475 cites W2124309573 @default.
- W4294991475 cites W2124798883 @default.
- W4294991475 cites W2125150759 @default.
- W4294991475 cites W2130300976 @default.
- W4294991475 cites W2132721917 @default.
- W4294991475 cites W2133639252 @default.
- W4294991475 cites W2134260485 @default.
- W4294991475 cites W2135363031 @default.
- W4294991475 cites W2137956968 @default.
- W4294991475 cites W2138914111 @default.
- W4294991475 cites W2144347190 @default.
- W4294991475 cites W2160859910 @default.
- W4294991475 cites W2163695915 @default.
- W4294991475 cites W2235679918 @default.
- W4294991475 cites W2253068377 @default.
- W4294991475 cites W2278269182 @default.
- W4294991475 cites W2301131921 @default.
- W4294991475 cites W2333398204 @default.
- W4294991475 cites W2345818435 @default.
- W4294991475 cites W2400615778 @default.
- W4294991475 cites W2409936513 @default.
- W4294991475 cites W2411711891 @default.
- W4294991475 cites W2464656850 @default.
- W4294991475 cites W2624837927 @default.
- W4294991475 cites W2625013350 @default.
- W4294991475 cites W2784278501 @default.
- W4294991475 cites W2795055822 @default.
- W4294991475 cites W2896532574 @default.
- W4294991475 cites W2909464755 @default.
- W4294991475 cites W2911835860 @default.
- W4294991475 cites W2979759357 @default.
- W4294991475 cites W2989388815 @default.
- W4294991475 cites W3008025512 @default.
- W4294991475 cites W3045693682 @default.
- W4294991475 cites W3125905406 @default.
- W4294991475 cites W3131106424 @default.
- W4294991475 cites W3177478456 @default.
- W4294991475 cites W3207367722 @default.
- W4294991475 cites W4211056322 @default.
- W4294991475 cites W4211144794 @default.
- W4294991475 cites W4220966082 @default.
- W4294991475 cites W4232133563 @default.
- W4294991475 cites W77445965 @default.
- W4294991475 doi "https://doi.org/10.14814/phy2.15340" @default.
- W4294991475 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36073059" @default.
- W4294991475 hasPublicationYear "2022" @default.
- W4294991475 type Work @default.
- W4294991475 citedByCount "0" @default.
- W4294991475 crossrefType "journal-article" @default.
- W4294991475 hasAuthorship W4294991475A5003544851 @default.
- W4294991475 hasAuthorship W4294991475A5022422924 @default.
- W4294991475 hasAuthorship W4294991475A5023131199 @default.
- W4294991475 hasAuthorship W4294991475A5036535003 @default.
- W4294991475 hasAuthorship W4294991475A5046834672 @default.