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• W4295008001 endingPage "340360" @default.
• W4295008001 startingPage "340360" @default.
• W4295008001 abstract "Infectious diseases caused by viruses have attracted global concern owing to their rapid spread and catastrophic consequences. Therefore, developing fast and reliable on-site virus detection methods is essential for the prevention and treatment of virus-related diseases. In this study, immunoassays on a membrane, combining virus preconcentration with nanoparticle-based signal amplification, were used to realize the rapid and accurate visual detection of viruses. The biotin–streptavidin scaffolds for target virus preconcentration were established on a membrane, and subsequently a Zika aptamer (Apt) immobilized on the membrane recognized and captured the nonstructural protein 1 of Zika virus (ZIKV-NS1). The probe for detection was synthesized by conjugating the Zika Apt with a high level of horseradish peroxidase on gold nanoparticles. The ZIKV-loaded membrane was incubated with the probes, and the viral signal was amplified as the signal of horseradish peroxidase. In the presence of 3,3,5′,5′-tetramethyl benzidine and hydrogen peroxide, the green color of the probe-coated membrane indicated the level of ZIKV-NS1. Our developed method could reach a detection limit of 5 ng mL−1, and the whole procedure could be completed within 1 h. Analyses of rabbit serum and environmental water samples demonstrated that an immunoassay-based approach on the membrane could accurately determine the level of ZIKV-NS1 against the complicated matrix. Our results suggest that this virus detection method has a high potential for application in clinical and environmental settings." @default.
• W4295008001 created "2022-09-09" @default.
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• W4295008001 date "2022-10-01" @default.
• W4295008001 modified "2023-09-24" @default.
• W4295008001 title "Developing a robust method integrating with selective membrane-based preconcentration and signal amplification for field virus detection" @default.
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• W4295008001 doi "https://doi.org/10.1016/j.aca.2022.340360" @default.
• W4295008001 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36156222" @default.
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