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- W4295095247 abstract "Abstract The antigenicity of SARS-CoV-2 is a critical issue for the effectiveness of the vaccine, and thus it should be phenotypically evaluated by serological assays as new field isolates emerge. The hemagglutination/hemagglutination-inhibition (HA/HI) tests are well-known as a representative method for antigenic analysis of influenza viruses, but SARS-CoV-2 is unlikely to agglutinate to human or guinea pig red blood cells. Therefore, the antigenic analysis requires complicated enzyme-linked immunosorbent assay (ELISA) or cell-based assays such as the microneutralization assay. In this study, we developed the particle agglutination/particle agglutination-inhibition (PA/PAI) test to easily and rapidly quantify the virus and antibody using human angiotensin-converting enzyme 2 (hACE2)-bound latex beads. The PA titer was positively correlated with the plaque-forming units. The PAI titer using post-infection Syrian hamster antisera clearly revealed the antigenic difference between the omicron and previous variants. The results show the PAI test is useful for easy and rapid antigenic analysis of SARS-CoV-2." @default.
- W4295095247 created "2022-09-10" @default.
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- W4295095247 date "2022-09-09" @default.
- W4295095247 modified "2023-09-27" @default.
- W4295095247 title "Use of the particle agglutination/particle agglutination-inhibition test for antigenic analysis of SARS-CoV-2" @default.
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- W4295095247 doi "https://doi.org/10.1101/2022.09.08.507221" @default.
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