FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4295308796> ?p ?o ?g. }

• W4295308796 abstract "Fucoxanthin is a major light-harvesting pigment in ecologically important algae such as diatoms, haptophytes, and brown algae (Phaeophyceae). Therefore, it is a major driver of global primary productivity. Species of these algal groups are brown colored because the high amounts of fucoxanthin bound to the proteins of their photosynthetic machineries enable efficient absorption of green light. While the structure of these fucoxanthin-chlorophyll proteins has recently been resolved, the biosynthetic pathway of fucoxanthin is still unknown. Here, we identified two enzymes central to this pathway by generating corresponding knockout mutants of the diatom Phaeodactylum tricornutum that are green due to the lack of fucoxanthin. Complementation of the mutants with the native genes or orthologs from haptophytes restored fucoxanthin biosynthesis. We propose a complete biosynthetic path to fucoxanthin in diatoms and haptophytes based on the carotenoid intermediates identified in the mutants and in vitro biochemical assays. It is substantially more complex than anticipated and reveals diadinoxanthin metabolism as the central regulatory hub connecting the photoprotective xanthophyll cycle and the formation of fucoxanthin. Moreover, our data show that the pathway evolved by repeated duplication and neofunctionalization of genes for the xanthophyll cycle enzymes violaxanthin de-epoxidase and zeaxanthin epoxidase. Brown algae lack diadinoxanthin and the genes described here and instead use an alternative pathway predicted to involve fewer enzymes. Our work represents a major step forward in elucidating the biosynthesis of fucoxanthin and understanding the evolution, biogenesis, and regulation of the photosynthetic machinery in algae." @default.
• W4295308796 created "2022-09-12" @default.
• W4295308796 creator A5004180106 @default.
• W4295308796 creator A5005935590 @default.
• W4295308796 creator A5008118658 @default.
• W4295308796 creator A5008921132 @default.
• W4295308796 creator A5012940856 @default.
• W4295308796 creator A5013375641 @default.
• W4295308796 creator A5024120345 @default.
• W4295308796 creator A5046298715 @default.
• W4295308796 creator A5070707262 @default.
• W4295308796 creator A5072569510 @default.
• W4295308796 creator A5074424310 @default.
• W4295308796 creator A5077343968 @default.
• W4295308796 creator A5078283824 @default.
• W4295308796 creator A5081326616 @default.
• W4295308796 creator A5083532068 @default.
• W4295308796 date "2022-09-12" @default.
• W4295308796 modified "2023-09-30" @default.
• W4295308796 title "Green diatom mutants reveal an intricate biosynthetic pathway of fucoxanthin" @default.
• W4295308796 cites W13725583 @default.
• W4295308796 cites W1485497108 @default.
• W4295308796 cites W1501460217 @default.
• W4295308796 cites W1549539801 @default.
• W4295308796 cites W1616428661 @default.
• W4295308796 cites W1720106642 @default.
• W4295308796 cites W1881066983 @default.
• W4295308796 cites W1958499792 @default.
• W4295308796 cites W1963496118 @default.
• W4295308796 cites W1968657515 @default.
• W4295308796 cites W1969818846 @default.
• W4295308796 cites W1973757188 @default.
• W4295308796 cites W1975135854 @default.
• W4295308796 cites W1984701121 @default.
• W4295308796 cites W1988780133 @default.
• W4295308796 cites W1989922725 @default.
• W4295308796 cites W1994592931 @default.
• W4295308796 cites W1995800359 @default.
• W4295308796 cites W2016123825 @default.
• W4295308796 cites W2019619926 @default.
• W4295308796 cites W2025366392 @default.
• W4295308796 cites W2026516661 @default.
• W4295308796 cites W2034403867 @default.
• W4295308796 cites W2038226984 @default.
• W4295308796 cites W2040305620 @default.
• W4295308796 cites W2050470820 @default.
• W4295308796 cites W2054970975 @default.
• W4295308796 cites W2061340909 @default.
• W4295308796 cites W2062018285 @default.
• W4295308796 cites W2063651453 @default.
• W4295308796 cites W2064941324 @default.
• W4295308796 cites W2074506764 @default.
• W4295308796 cites W2079492081 @default.
• W4295308796 cites W2094201044 @default.
• W4295308796 cites W2095724872 @default.
• W4295308796 cites W2098140159 @default.
• W4295308796 cites W2100425347 @default.
• W4295308796 cites W2104349772 @default.
• W4295308796 cites W2112073484 @default.
• W4295308796 cites W2119177774 @default.
• W4295308796 cites W2127099340 @default.
• W4295308796 cites W2127847431 @default.
• W4295308796 cites W2129095580 @default.
• W4295308796 cites W2141052558 @default.
• W4295308796 cites W2142888309 @default.
• W4295308796 cites W2147132217 @default.
• W4295308796 cites W2150297520 @default.
• W4295308796 cites W2155923306 @default.
• W4295308796 cites W2158714788 @default.
• W4295308796 cites W2159958980 @default.
• W4295308796 cites W2161324059 @default.
• W4295308796 cites W2161746138 @default.
• W4295308796 cites W2162371815 @default.
• W4295308796 cites W2167705142 @default.
• W4295308796 cites W2259522006 @default.
• W4295308796 cites W2290413657 @default.
• W4295308796 cites W2306748636 @default.
• W4295308796 cites W2343659782 @default.
• W4295308796 cites W2758941834 @default.
• W4295308796 cites W2768571346 @default.
• W4295308796 cites W2782004254 @default.
• W4295308796 cites W2803479625 @default.
• W4295308796 cites W2891772303 @default.
• W4295308796 cites W2892586303 @default.
• W4295308796 cites W2901445771 @default.
• W4295308796 cites W2912557076 @default.
• W4295308796 cites W2947563222 @default.
• W4295308796 cites W2954061271 @default.
• W4295308796 cites W2966137395 @default.
• W4295308796 cites W2966277946 @default.
• W4295308796 cites W2973064791 @default.
• W4295308796 cites W2983319040 @default.
• W4295308796 cites W2996972459 @default.
• W4295308796 cites W3010584835 @default.
• W4295308796 cites W3024872414 @default.
• W4295308796 cites W3092470966 @default.
• W4295308796 cites W3168511271 @default.
• W4295308796 cites W3200553754 @default.
• W4295308796 cites W4225414448 @default.
• W4295308796 cites W4253572634 @default.

• next