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- W4295514274 abstract "Many bacterial species are represented by a pan-genome, whose genetic repertoire far outstrips that of any single bacterial genome. Here we investigate how a bacterial pan-genome might influence gene essentiality and whether essential genes that are initially critical for the survival of an organism can evolve to become non-essential. By using Transposon insertion sequencing (Tn-seq), whole-genome sequencing and RNA-seq on a set of 36 clinical Streptococcus pneumoniae strains representative of >68% of the species' pan-genome, we identify a species-wide 'essentialome' that can be subdivided into universal, core strain-specific and accessory essential genes. By employing 'forced-evolution experiments', we show that specific genetic changes allow bacteria to bypass essentiality. Moreover, by untangling several genetic mechanisms, we show that gene essentiality can be highly influenced by and/or be dependent on: (1) the composition of the accessory genome, (2) the accumulation of toxic intermediates, (3) functional redundancy, (4) efficient recycling of critical metabolites and (5) pathway rewiring. While this functional characterization underscores the evolvability potential of many essential genes, we also show that genes with differential essentiality remain important antimicrobial drug target candidates, as their inactivation almost always has a severe fitness cost in vivo." @default.
- W4295514274 created "2022-09-14" @default.
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- W4295514274 date "2022-09-12" @default.
- W4295514274 modified "2023-10-15" @default.
- W4295514274 title "A bacterial pan-genome makes gene essentiality strain-dependent and evolvable" @default.
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- W4295514274 cites W1999574084 @default.
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- W4295514274 cites W2008074150 @default.
- W4295514274 cites W2012457803 @default.
- W4295514274 cites W2015616512 @default.
- W4295514274 cites W2020247237 @default.
- W4295514274 cites W2030924818 @default.
- W4295514274 cites W2038992687 @default.
- W4295514274 cites W2055079545 @default.
- W4295514274 cites W2058989497 @default.
- W4295514274 cites W2085454969 @default.
- W4295514274 cites W2087631453 @default.
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- W4295514274 cites W2143334851 @default.
- W4295514274 cites W2152311269 @default.
- W4295514274 cites W2153125716 @default.
- W4295514274 cites W2168571698 @default.
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- W4295514274 cites W2515246605 @default.
- W4295514274 cites W2535283300 @default.
- W4295514274 cites W2546667113 @default.
- W4295514274 cites W2613730347 @default.
- W4295514274 cites W2735876928 @default.
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- W4295514274 cites W2937635380 @default.
- W4295514274 cites W2940289922 @default.
- W4295514274 cites W2949102320 @default.
- W4295514274 cites W2950731393 @default.
- W4295514274 cites W2952702265 @default.
- W4295514274 cites W2961135345 @default.
- W4295514274 cites W2970828853 @default.
- W4295514274 cites W2980087843 @default.
- W4295514274 cites W3012393121 @default.
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- W4295514274 doi "https://doi.org/10.1038/s41564-022-01208-7" @default.
- W4295514274 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36097170" @default.
- W4295514274 hasPublicationYear "2022" @default.
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