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- W4295693172 abstract "Human chromosomes are pervasively transcribed, but systematic understanding of coding and long noncoding RNA (lncRNA) genome function in cell differentiation is lacking. Using CRISPR interference (CRISPRi) in human induced pluripotent stem cells, we performed dual genome-wide screens—assessing 18,905 protein-coding and 10,678 lncRNA loci—and identified 419 coding and 201 lncRNA genes that regulate neural induction. Integrative analyses revealed distinct properties of coding and lncRNA genome function, including a 10-fold enrichment of lncRNA genes for roles in differentiation compared with proliferation. Further, we applied CRISPRi perturbation coupled with single-cell RNA-seq (Perturb-seq) to obtain granular insights into neural induction phenotypes. While most coding hits stalled or aborted differentiation, lncRNA hits were enriched for the genesis of diverse cellular states, including those outside the neural lineage. In addition to providing a rich resource for understanding coding and lncRNA gene function in development, these results indicate that the lncRNA genome regulates lineage commitment in a manner fundamentally distinct from coding genes." @default.
- W4295693172 created "2022-09-14" @default.
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- W4295693172 date "2022-11-01" @default.
- W4295693172 modified "2023-10-16" @default.
- W4295693172 title "Dual genome-wide coding and lncRNA screens in neural induction of induced pluripotent stem cells" @default.
- W4295693172 cites W1594675904 @default.
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- W4295693172 cites W1934704664 @default.
- W4295693172 cites W1970741980 @default.
- W4295693172 cites W1976459963 @default.
- W4295693172 cites W1981088384 @default.
- W4295693172 cites W1984173288 @default.
- W4295693172 cites W1985567838 @default.
- W4295693172 cites W2008695914 @default.
- W4295693172 cites W2015845481 @default.
- W4295693172 cites W2017844161 @default.
- W4295693172 cites W2022936129 @default.
- W4295693172 cites W2032183472 @default.
- W4295693172 cites W2041801261 @default.
- W4295693172 cites W2074495242 @default.
- W4295693172 cites W2087786585 @default.
- W4295693172 cites W2088069070 @default.
- W4295693172 cites W2096753123 @default.
- W4295693172 cites W2105559737 @default.
- W4295693172 cites W2106296224 @default.
- W4295693172 cites W2121828161 @default.
- W4295693172 cites W2130313087 @default.
- W4295693172 cites W2134033103 @default.
- W4295693172 cites W2137840425 @default.
- W4295693172 cites W2138207763 @default.
- W4295693172 cites W2138884188 @default.
- W4295693172 cites W2140140367 @default.
- W4295693172 cites W2141831115 @default.
- W4295693172 cites W2152505144 @default.
- W4295693172 cites W2153268494 @default.
- W4295693172 cites W2153773863 @default.
- W4295693172 cites W2157232603 @default.
- W4295693172 cites W2166810745 @default.
- W4295693172 cites W2171808845 @default.
- W4295693172 cites W2179438025 @default.
- W4295693172 cites W2180291937 @default.
- W4295693172 cites W2184997675 @default.
- W4295693172 cites W2197124664 @default.
- W4295693172 cites W2295326929 @default.
- W4295693172 cites W2323326409 @default.
- W4295693172 cites W2341539131 @default.
- W4295693172 cites W2510875395 @default.
- W4295693172 cites W2511664109 @default.
- W4295693172 cites W2514110482 @default.
- W4295693172 cites W2523099503 @default.
- W4295693172 cites W2540381357 @default.
- W4295693172 cites W2548201800 @default.
- W4295693172 cites W2561535772 @default.
- W4295693172 cites W2561754210 @default.
- W4295693172 cites W2562947047 @default.
- W4295693172 cites W2577994754 @default.
- W4295693172 cites W2593488635 @default.
- W4295693172 cites W2620256678 @default.
- W4295693172 cites W2727479110 @default.
- W4295693172 cites W2749564414 @default.
- W4295693172 cites W2751596777 @default.
- W4295693172 cites W2753497899 @default.
- W4295693172 cites W2760692740 @default.
- W4295693172 cites W2766437506 @default.
- W4295693172 cites W2784570320 @default.
- W4295693172 cites W2800773898 @default.
- W4295693172 cites W2902716564 @default.
- W4295693172 cites W2949177718 @default.
- W4295693172 cites W2951184828 @default.
- W4295693172 cites W2951448241 @default.
- W4295693172 cites W2981212141 @default.
- W4295693172 cites W3013900499 @default.
- W4295693172 cites W3015892555 @default.
- W4295693172 cites W3022572641 @default.
- W4295693172 cites W3047141442 @default.
- W4295693172 cites W3092857332 @default.
- W4295693172 cites W3110610967 @default.
- W4295693172 cites W3180701922 @default.
- W4295693172 cites W4224222925 @default.
- W4295693172 cites W4233582454 @default.
- W4295693172 cites W795089952 @default.
- W4295693172 doi "https://doi.org/10.1016/j.xgen.2022.100177" @default.
- W4295693172 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36381608" @default.