Matches in SemOpenAlex for { <https://semopenalex.org/work/W4295957079> ?p ?o ?g. }
- W4295957079 endingPage "11" @default.
- W4295957079 startingPage "1" @default.
- W4295957079 abstract "This study was designed to explore the role and mechanism of eukaryotic initiation factor 3C (EIF3C) in the proliferation and apoptosis of lung cancer cells.EIF3C expression in clinic lung cancer tissues was detected by immunohistochemistry assay. Cell transfection with lentivirus EIF3C short hairpin RNA (shRNA) was performed with Lipofectamine 2000. Cell proliferation was evaluated by Celigo and MTT assays. Caspase-3/7 activity was assessed using caspase-3/7 assay kit for cell apoptosis detection. The apoptosis rate of lung cancer cells was assessed by flow cytometry. A transplanted tumor nude-mouse model was established to clarify the role of EIF3C in lung cancer. The potential mechanism of EIF3C was explored by mRNA microarray analysis. Among the top 30 up- and downregulated mRNAs selected for RT-qPCR, 5 were chosen for western blot analysis.EIF3C was abnormally overexpressed in lung cancer cell lines and tissues. Silencing EIF3C suppressed the proliferation and promoted the apoptosis of lung cancer cells. In vivo experiments using transplanted tumor nude-mouse model suggested that EIF3C promoted lung cancer tumorigenesis. Further, mRNA microarray analyses identified 189 upregulated and 83 downregulated differentially expressed mRNA between the KD and negative control groups. After validation by RT-qPCR and western blot, three downstream genes (APP, HSPA1A, and LMNB1) were confirmed.EIF3C overexpression may facilitate the proliferation and hamper the apoptosis of lung cancer cells by regulating the APP/HSPA1A/LMNB1 axis." @default.
- W4295957079 created "2022-09-16" @default.
- W4295957079 creator A5011198695 @default.
- W4295957079 creator A5014567396 @default.
- W4295957079 creator A5033194571 @default.
- W4295957079 creator A5037258091 @default.
- W4295957079 creator A5047030779 @default.
- W4295957079 creator A5064487127 @default.
- W4295957079 creator A5070265768 @default.
- W4295957079 creator A5081816315 @default.
- W4295957079 date "2022-09-14" @default.
- W4295957079 modified "2023-09-26" @default.
- W4295957079 title "EIF3C Promotes Lung Cancer Tumorigenesis by Regulating the APP/HSPA1A/LMNB1 Axis" @default.
- W4295957079 cites W1245506155 @default.
- W4295957079 cites W1591236566 @default.
- W4295957079 cites W1677807414 @default.
- W4295957079 cites W1978068592 @default.
- W4295957079 cites W2002166179 @default.
- W4295957079 cites W2004526658 @default.
- W4295957079 cites W2009427803 @default.
- W4295957079 cites W2015062291 @default.
- W4295957079 cites W2016803661 @default.
- W4295957079 cites W2018340043 @default.
- W4295957079 cites W2030249003 @default.
- W4295957079 cites W2096603980 @default.
- W4295957079 cites W2114295583 @default.
- W4295957079 cites W2127394968 @default.
- W4295957079 cites W2140342181 @default.
- W4295957079 cites W2171735284 @default.
- W4295957079 cites W2480295405 @default.
- W4295957079 cites W2551770263 @default.
- W4295957079 cites W2590180796 @default.
- W4295957079 cites W2593792882 @default.
- W4295957079 cites W2783044155 @default.
- W4295957079 cites W2784371446 @default.
- W4295957079 cites W2802859292 @default.
- W4295957079 cites W2809309222 @default.
- W4295957079 cites W2884895372 @default.
- W4295957079 cites W2898896996 @default.
- W4295957079 cites W2945919866 @default.
- W4295957079 cites W2952896494 @default.
- W4295957079 cites W2980850559 @default.
- W4295957079 cites W2984070189 @default.
- W4295957079 cites W2992033410 @default.
- W4295957079 cites W2994755653 @default.
- W4295957079 cites W2997487510 @default.
- W4295957079 cites W3017062441 @default.
- W4295957079 cites W3086675276 @default.
- W4295957079 cites W3119066084 @default.
- W4295957079 cites W3126287530 @default.
- W4295957079 cites W3139095723 @default.
- W4295957079 cites W2992449806 @default.
- W4295957079 doi "https://doi.org/10.1155/2022/9464094" @default.
- W4295957079 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36157221" @default.
- W4295957079 hasPublicationYear "2022" @default.
- W4295957079 type Work @default.
- W4295957079 citedByCount "1" @default.
- W4295957079 countsByYear W42959570792022 @default.
- W4295957079 crossrefType "journal-article" @default.
- W4295957079 hasAuthorship W4295957079A5011198695 @default.
- W4295957079 hasAuthorship W4295957079A5014567396 @default.
- W4295957079 hasAuthorship W4295957079A5033194571 @default.
- W4295957079 hasAuthorship W4295957079A5037258091 @default.
- W4295957079 hasAuthorship W4295957079A5047030779 @default.
- W4295957079 hasAuthorship W4295957079A5064487127 @default.
- W4295957079 hasAuthorship W4295957079A5070265768 @default.
- W4295957079 hasAuthorship W4295957079A5081816315 @default.
- W4295957079 hasBestOaLocation W42959570791 @default.
- W4295957079 hasConcept C104317684 @default.
- W4295957079 hasConcept C119056186 @default.
- W4295957079 hasConcept C121608353 @default.
- W4295957079 hasConcept C142724271 @default.
- W4295957079 hasConcept C153911025 @default.
- W4295957079 hasConcept C190283241 @default.
- W4295957079 hasConcept C2776256026 @default.
- W4295957079 hasConcept C2776415932 @default.
- W4295957079 hasConcept C2780278673 @default.
- W4295957079 hasConcept C502942594 @default.
- W4295957079 hasConcept C54355233 @default.
- W4295957079 hasConcept C55493867 @default.
- W4295957079 hasConcept C555283112 @default.
- W4295957079 hasConcept C62112901 @default.
- W4295957079 hasConcept C71924100 @default.
- W4295957079 hasConcept C86803240 @default.
- W4295957079 hasConceptScore W4295957079C104317684 @default.
- W4295957079 hasConceptScore W4295957079C119056186 @default.
- W4295957079 hasConceptScore W4295957079C121608353 @default.
- W4295957079 hasConceptScore W4295957079C142724271 @default.
- W4295957079 hasConceptScore W4295957079C153911025 @default.
- W4295957079 hasConceptScore W4295957079C190283241 @default.
- W4295957079 hasConceptScore W4295957079C2776256026 @default.
- W4295957079 hasConceptScore W4295957079C2776415932 @default.
- W4295957079 hasConceptScore W4295957079C2780278673 @default.
- W4295957079 hasConceptScore W4295957079C502942594 @default.
- W4295957079 hasConceptScore W4295957079C54355233 @default.
- W4295957079 hasConceptScore W4295957079C55493867 @default.
- W4295957079 hasConceptScore W4295957079C555283112 @default.
- W4295957079 hasConceptScore W4295957079C62112901 @default.