FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4296002913> ?p ?o ?g. }

• W4296002913 endingPage "10603" @default.
• W4296002913 startingPage "10603" @default.
• W4296002913 abstract "Porcine epidemic diarrhea virus (PEDV) infection causes watery diarrhea and vomiting in piglets. The pathogenesis of PEDV infection is related to intestinal inflammation. It is known that 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) has potent anti-inflammatory activity, but it is unknown whether 1,25(OH)2D3 can inhibit the PEDV-induced inflammatory response and the underlying mechanism. We used transcriptome analysis, gene and protein expression, RNA interference and overexpression, and other techniques to study the anti-inflammatory effects of 1,25(OH)2D3 on PEDV infection in IPEC-J2 cells. The results showed that interleukin 19 (IL-19) and C-C motif chemokine ligand 20 (CCL20) gene expression were enhanced with the increase in PEDV infection time in IPEC-J2 cells. Interestingly, 1,25(OH)2D3 supplementation obviously inhibited IL-19 and CCL20 expression induced by PEDV. Meanwhile, we also found that 1,25(OH)2D3 reduced p-NF-κB, p-STAT1, and p-STAT3 protein levels induced by PEDV at 24 h post-infection. IκBα and SOCS3, NF-κB, and STAT inhibitor respectively, were increased by 1,25(OH)2D3 supplementation upon PEDV infection. In addition, 1,25(OH)2D3 supplementation inhibited ISG15 and MxA expression induced by PEDV. Although 1,25(OH)2D3 suppressed the JAK/STAT signal pathway and antiviral gene expression, it had no significant effects on PEDV replication and IFN-α-induced antiviral effects. In addition, when the vitamin D receptor (VDR) was silenced by siRNA, the anti-inflammatory effect of 1,25(OH)2D3 was inhibited. Meanwhile, the overexpression of VDR significantly downregulated IL-19 and CCL20 expression induced by PEDV infection. Together, our results provide powerful evidence that 1,25(OH)2D3 could alleviate PEDV-induced inflammation by regulating the NF-κB and JAK/STAT signaling pathways through VDR. These results suggest that vitamin D could contribute to inhibiting intestinal inflammation and alleviating intestinal damage in PEDV-infected piglets, which offers new approaches for the development of nutritional strategies to prevent PEDV infection in piglets." @default.
• W4296002913 created "2022-09-16" @default.
• W4296002913 creator A5004468414 @default.
• W4296002913 creator A5005388620 @default.
• W4296002913 creator A5006216982 @default.
• W4296002913 creator A5014633542 @default.
• W4296002913 creator A5023809939 @default.
• W4296002913 creator A5030188489 @default.
• W4296002913 creator A5043632436 @default.
• W4296002913 creator A5049317673 @default.
• W4296002913 creator A5059581347 @default.
• W4296002913 creator A5063659373 @default.
• W4296002913 creator A5072617804 @default.
• W4296002913 creator A5085375811 @default.
• W4296002913 creator A5089540321 @default.
• W4296002913 date "2022-09-13" @default.
• W4296002913 modified "2023-10-05" @default.
• W4296002913 title "1,25-Dihydroxyvitamin D3 Negatively Regulates the Inflammatory Response to Porcine Epidemic Diarrhea Virus Infection by Inhibiting NF-κB and JAK/STAT Signaling Pathway in IPEC-J2 Porcine Epithelial Cells" @default.
• W4296002913 cites W1556684910 @default.
• W4296002913 cites W1970308473 @default.
• W4296002913 cites W1970516100 @default.
• W4296002913 cites W1971724873 @default.
• W4296002913 cites W1973454407 @default.
• W4296002913 cites W1985160576 @default.
• W4296002913 cites W1998577849 @default.
• W4296002913 cites W2005817924 @default.
• W4296002913 cites W2012504769 @default.
• W4296002913 cites W2031375858 @default.
• W4296002913 cites W2038621270 @default.
• W4296002913 cites W2043045955 @default.
• W4296002913 cites W2056297553 @default.
• W4296002913 cites W2077574194 @default.
• W4296002913 cites W2091344524 @default.
• W4296002913 cites W2091957879 @default.
• W4296002913 cites W2097957152 @default.
• W4296002913 cites W2099126377 @default.
• W4296002913 cites W2113290646 @default.
• W4296002913 cites W2117075813 @default.
• W4296002913 cites W2117263637 @default.
• W4296002913 cites W2124927273 @default.
• W4296002913 cites W2131923232 @default.
• W4296002913 cites W2133570354 @default.
• W4296002913 cites W2144052431 @default.
• W4296002913 cites W2151565551 @default.
• W4296002913 cites W2152532651 @default.
• W4296002913 cites W2155729092 @default.
• W4296002913 cites W2159979956 @default.
• W4296002913 cites W2254563264 @default.
• W4296002913 cites W2290389478 @default.
• W4296002913 cites W2292371441 @default.
• W4296002913 cites W2411301702 @default.
• W4296002913 cites W2529149698 @default.
• W4296002913 cites W2618503740 @default.
• W4296002913 cites W2758443375 @default.
• W4296002913 cites W2808118052 @default.
• W4296002913 cites W2890795854 @default.
• W4296002913 cites W2969423276 @default.
• W4296002913 cites W2971306975 @default.
• W4296002913 cites W2990191413 @default.
• W4296002913 cites W3006155236 @default.
• W4296002913 cites W3016171630 @default.
• W4296002913 cites W3018199512 @default.
• W4296002913 cites W3171713403 @default.
• W4296002913 doi "https://doi.org/10.3390/ijms231810603" @default.
• W4296002913 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36142545" @default.
• W4296002913 hasPublicationYear "2022" @default.
• W4296002913 type Work @default.
• W4296002913 citedByCount "1" @default.
• W4296002913 countsByYear W42960029132023 @default.
• W4296002913 crossrefType "journal-article" @default.
• W4296002913 hasAuthorship W4296002913A5004468414 @default.
• W4296002913 hasAuthorship W4296002913A5005388620 @default.
• W4296002913 hasAuthorship W4296002913A5006216982 @default.
• W4296002913 hasAuthorship W4296002913A5014633542 @default.
• W4296002913 hasAuthorship W4296002913A5023809939 @default.
• W4296002913 hasAuthorship W4296002913A5030188489 @default.
• W4296002913 hasAuthorship W4296002913A5043632436 @default.
• W4296002913 hasAuthorship W4296002913A5049317673 @default.
• W4296002913 hasAuthorship W4296002913A5059581347 @default.
• W4296002913 hasAuthorship W4296002913A5063659373 @default.
• W4296002913 hasAuthorship W4296002913A5072617804 @default.
• W4296002913 hasAuthorship W4296002913A5085375811 @default.
• W4296002913 hasAuthorship W4296002913A5089540321 @default.
• W4296002913 hasBestOaLocation W42960029131 @default.
• W4296002913 hasConcept C104317684 @default.
• W4296002913 hasConcept C112392421 @default.
• W4296002913 hasConcept C12823836 @default.
• W4296002913 hasConcept C13373296 @default.
• W4296002913 hasConcept C145430368 @default.
• W4296002913 hasConcept C157333092 @default.
• W4296002913 hasConcept C159047783 @default.
• W4296002913 hasConcept C161238802 @default.
• W4296002913 hasConcept C170493617 @default.
• W4296002913 hasConcept C179639408 @default.
• W4296002913 hasConcept C191732599 @default.
• W4296002913 hasConcept C195794163 @default.
• W4296002913 hasConcept C203014093 @default.
• W4296002913 hasConcept C2522874641 @default.

• next