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- W4296082208 abstract "Human embryonic stem cells (hESCs) continuously self-renew in culture and can be induced to differentiate into multiple cell types, including neural progenitor cells (NPCs). Here, we present a protocol to perform a CRISPR-Cas9 screen in hESCs to identify regulators that promote SOX1 expression during NPC formation. This screening protocol can be adapted with other endpoint reporters for the identification of genes involved in the commitment of hESCs to other cell lineages. For complete details on the use and execution of this protocol, please refer to Sivakumar et al. (2022)." @default.
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- W4296082208 date "2022-12-01" @default.
- W4296082208 modified "2023-09-30" @default.
- W4296082208 title "CRISPR-Cas9 screen in human embryonic stem cells to identify genes required for neural differentiation" @default.
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- W4296082208 doi "https://doi.org/10.1016/j.xpro.2022.101682" @default.
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