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- W4296103977 abstract "A three-dimensional (3D) tumor spheroid model plays a critical role in mimicking tumor microenvironments in vivo. However, the conventional culture methods lack the ability to manipulate the 3D tumor spheroids in a homogeneous manner. To address this limitation, we developed a microfluidic-based droplet system for drug screening applications. We used a tree-shaped gradient generator to control the cell density and encapsulate the cells within uniform-sized droplets to generate a 3D gradient-sized tumor spheroid. Using this microfluidic-based droplet system, we demonstrated the high-throughput generation of uniform 3D tumor spheroids containing various cellular ratios for the analysis of the anti-cancer drug cytotoxicity. Consequently, this microfluidic-based gradient droplet generator could be a potentially powerful tool for anti-cancer drug screening applications." @default.
- W4296103977 created "2022-09-17" @default.
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- W4296103977 date "2022-09-08" @default.
- W4296103977 modified "2023-09-28" @default.
- W4296103977 title "3D Multicellular Tumor Spheroids in a Microfluidic Droplet System for Investigation of Drug Resistance" @default.
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- W4296103977 doi "https://doi.org/10.3390/polym14183752" @default.
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