FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4296196908> ?p ?o ?g. }

• W4296196908 endingPage "873" @default.
• W4296196908 startingPage "864" @default.
• W4296196908 abstract "To study the prevalence of spermatogonia in adult subjects with Klinefelter syndrome (KS) using MAGE-A4 and UCHL1 (PGP9.5) immunohistochemistry as markers for undifferentiated spermatogonial cells. We aimed to compare this method to the gold standard of hematoxylin and eosin (H & E) staining with histologic analysis in the largest reported cohort of adult subjects with KS.A retrospective cohort study.Infertility Clinic and Institute for Regenerative Medicine.This study consisted of 79 adult subjects with KS and 12 adult control subjects.The subjects with KS (n = 79) underwent bilateral testicular biopsy in an initial effort to recover spermatozoa for in vitro fertilization and intracytoplasmic sperm injection. The institutional review board approved the use of a portion of the archived diagnostic pathology paraffin blocks for the study. The samples were superimposed onto microscopic slides and labeled with the PGP9.5 and MAGE-A4 antibodies. Subjects (n = 12) who had previously consented to be organ donors via the National Disease Research Interchange were selected as controls. Dedicated genitourinary pathologists examined the H & E-, PGP9.5-, and MAGE-A4-stained tissue for presence of undifferentiated spermatogonia and spermatozoa with the use of a virtual microscopy software.The primary outcome was the presence of MAGE-A4-positive or UCHL1-positive tubules that indicate undifferentiated spermatogonia. Supportive outcomes include assessing the biopsy specimen for the following: total surface area; total seminiferous tubule surface area; total interstitium surface area; the total number of seminiferous tubules; and MAGE-A4- negative or UCHL1-negative tubules. Additionally, clinical information, such as age, karyotype, height, weight, mean testicle size, and hormonal panel (luteinizing hormone, follicle-stimulating hormone, and testosterone), was obtained and used in a single and multivariable analysis with linear regression to determine predictive factors for the number of UCHL1-positive tubules.The mean age of the subjects in the KS group was 32.9 ± 0.7 years (range, 16-48). UCHL1 (PGP9.5) and MAGE-A4 staining showed that 74.7% (n = 59) and 40.5% (n = 32) of the subjects with KS, respectively, were positive for undifferentiated spermatogonia compared with 100% (n = 12) of the control subjects who were positive for both the markers. Hematoxylin and eosin with microscopic analysis showed that only 10.1% (n = 8) of the subjects were positive for spermatogonia. The mean number of positive tubules per subject with KS was 11.8 ± 1.8 for UCHL1 and 3.7 ± 1.0 for MAGE-A4. Secondary analysis showed 7 (8.9%) adult subjects with KS as positive for spermatozoa on biopsy. The population having negative testicular sperm extraction results (n = 72) showed a spermatogonia-positive rate of 1.4%, (n = 1), 72.2% (n = 52), and 34.7% (n = 25) using H & E, UCHL1, and MAGE-A4, respectively. Further analysis showed that 54 (75.0%) subjects were either positive for UCHL1 or MAGE-A4. Twenty (27.8%) subjects were positive for both UCHL1 and MAGE-A4. Multivariate analysis with linear regression showed no significant correlation between clinical variables and the number of UCHL1-positive tubules found on biopsy specimens.We report a cohort of adult subjects with KS undergoing analysis for the presence of undifferentiated spermatogonia. UCHL1 and MAGE-A4 immunostaining appear to be an effective way of identifying undifferentiated spermatogonia in testicular biopsy specimens of subjects with KS. Despite observing deterioration in the testicular architecture, many patients remain positive for undifferentiated spermatogonia, which could be harvested and potentially used for infertility therapy in a patient with KS who is azoospermic and has negative testicular sperm extraction results." @default.
• W4296196908 created "2022-09-18" @default.
• W4296196908 creator A5004335359 @default.
• W4296196908 creator A5008750218 @default.
• W4296196908 creator A5018486819 @default.
• W4296196908 creator A5019933666 @default.
• W4296196908 creator A5027290610 @default.
• W4296196908 creator A5030131001 @default.
• W4296196908 creator A5030802910 @default.
• W4296196908 creator A5039364359 @default.
• W4296196908 creator A5062414050 @default.
• W4296196908 creator A5076544343 @default.
• W4296196908 creator A5082261321 @default.
• W4296196908 date "2022-11-01" @default.
• W4296196908 modified "2023-10-16" @default.
• W4296196908 title "Morphometric and immunohistochemical analysis as a method to identify undifferentiated spermatogonial cells in adult subjects with Klinefelter syndrome: a cohort study" @default.
• W4296196908 cites W1949032488 @default.
• W4296196908 cites W1985398262 @default.
• W4296196908 cites W2007668924 @default.
• W4296196908 cites W2053911546 @default.
• W4296196908 cites W2054743870 @default.
• W4296196908 cites W2077833546 @default.
• W4296196908 cites W2096312080 @default.
• W4296196908 cites W2104956810 @default.
• W4296196908 cites W2108934358 @default.
• W4296196908 cites W2110804381 @default.
• W4296196908 cites W2122040112 @default.
• W4296196908 cites W2124954943 @default.
• W4296196908 cites W2131397213 @default.
• W4296196908 cites W2136230328 @default.
• W4296196908 cites W2150982942 @default.
• W4296196908 cites W2170926399 @default.
• W4296196908 cites W2273933578 @default.
• W4296196908 cites W2274566290 @default.
• W4296196908 cites W2326088028 @default.
• W4296196908 cites W2341482240 @default.
• W4296196908 cites W2590403902 @default.
• W4296196908 cites W2604271309 @default.
• W4296196908 cites W2793713098 @default.
• W4296196908 cites W2801616121 @default.
• W4296196908 cites W2887748979 @default.
• W4296196908 cites W2995657008 @default.
• W4296196908 cites W3003640971 @default.
• W4296196908 cites W3186611731 @default.
• W4296196908 cites W4200148426 @default.
• W4296196908 doi "https://doi.org/10.1016/j.fertnstert.2022.07.015" @default.
• W4296196908 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36116982" @default.
• W4296196908 hasPublicationYear "2022" @default.
• W4296196908 type Work @default.
• W4296196908 citedByCount "4" @default.
• W4296196908 countsByYear W42961969082022 @default.
• W4296196908 countsByYear W42961969082023 @default.
• W4296196908 crossrefType "journal-article" @default.
• W4296196908 hasAuthorship W4296196908A5004335359 @default.
• W4296196908 hasAuthorship W4296196908A5008750218 @default.
• W4296196908 hasAuthorship W4296196908A5018486819 @default.
• W4296196908 hasAuthorship W4296196908A5019933666 @default.
• W4296196908 hasAuthorship W4296196908A5027290610 @default.
• W4296196908 hasAuthorship W4296196908A5030131001 @default.
• W4296196908 hasAuthorship W4296196908A5030802910 @default.
• W4296196908 hasAuthorship W4296196908A5039364359 @default.
• W4296196908 hasAuthorship W4296196908A5062414050 @default.
• W4296196908 hasAuthorship W4296196908A5076544343 @default.
• W4296196908 hasAuthorship W4296196908A5082261321 @default.
• W4296196908 hasConcept C123765429 @default.
• W4296196908 hasConcept C125473707 @default.
• W4296196908 hasConcept C126322002 @default.
• W4296196908 hasConcept C142724271 @default.
• W4296196908 hasConcept C16685009 @default.
• W4296196908 hasConcept C204232928 @default.
• W4296196908 hasConcept C2775934546 @default.
• W4296196908 hasConcept C2776314099 @default.
• W4296196908 hasConcept C2777338322 @default.
• W4296196908 hasConcept C2777688143 @default.
• W4296196908 hasConcept C2778054371 @default.
• W4296196908 hasConcept C2778278907 @default.
• W4296196908 hasConcept C2779234561 @default.
• W4296196908 hasConcept C2781068068 @default.
• W4296196908 hasConcept C54355233 @default.
• W4296196908 hasConcept C71924100 @default.
• W4296196908 hasConcept C72563966 @default.
• W4296196908 hasConcept C86803240 @default.
• W4296196908 hasConceptScore W4296196908C123765429 @default.
• W4296196908 hasConceptScore W4296196908C125473707 @default.
• W4296196908 hasConceptScore W4296196908C126322002 @default.
• W4296196908 hasConceptScore W4296196908C142724271 @default.
• W4296196908 hasConceptScore W4296196908C16685009 @default.
• W4296196908 hasConceptScore W4296196908C204232928 @default.
• W4296196908 hasConceptScore W4296196908C2775934546 @default.
• W4296196908 hasConceptScore W4296196908C2776314099 @default.
• W4296196908 hasConceptScore W4296196908C2777338322 @default.
• W4296196908 hasConceptScore W4296196908C2777688143 @default.
• W4296196908 hasConceptScore W4296196908C2778054371 @default.
• W4296196908 hasConceptScore W4296196908C2778278907 @default.
• W4296196908 hasConceptScore W4296196908C2779234561 @default.
• W4296196908 hasConceptScore W4296196908C2781068068 @default.
• W4296196908 hasConceptScore W4296196908C54355233 @default.
• W4296196908 hasConceptScore W4296196908C71924100 @default.

• next