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- W4296461946 abstract "ABSTRACT The lateral flow assay format enables rapid, instrument-free, at-home testing for SARS-CoV-2. Due to the absence of signal amplification, this simplicity comes at a cost in sensitivity. Here, we enhance sensitivity by developing an amplified lateral flow assay that incorporates isothermal, enzyme-free signal amplification based on the mechanism of hybridization chain reaction (HCR). The simplicity of the user experience is maintained using a disposable 3-channel lateral flow device to automatically deliver reagents to the test region in three successive stages without user interaction. To perform a test, the user loads the sample, closes the device, and reads the result by eye after 60 minutes. Detecting gamma-irradiated SARS-CoV-2 virions in a mixture of saliva and extraction buffer, the current amplified HCR lateral flow assay achieves a limit of detection of 200 copies/ μ L using available antibodies to target the SARS-CoV-2 nucleocapsid protein. By comparison, five commercial unamplified lateral flow assays that use proprietary antibodies exhibit limits of detection of 500 copies/ μ L, 1000 copies/ μ L, 2000 copies/ μ L, 2000 copies/ μ L, and 20,000 copies/ μ L. By swapping out antibody probes to target different pathogens, amplified HCR lateral flow assays offer a platform for simple, rapid, and sensitive at-home testing for infectious disease. As an alternative to viral protein detection, we further introduce an HCR lateral flow assay for viral RNA detection. HCR lateral flow assay Amplified Instrument-free At-home 60 min Naked eye SARS-CoV-2 200 copies/ μ L" @default.
- W4296461946 created "2022-09-20" @default.
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- W4296461946 date "2022-09-19" @default.
- W4296461946 modified "2023-10-03" @default.
- W4296461946 title "HCR Lateral Flow Assays for Amplified Instrument-Free At-Home SARS-CoV-2 Testing" @default.
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- W4296461946 doi "https://doi.org/10.1101/2022.09.18.508442" @default.
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