Matches in SemOpenAlex for { <https://semopenalex.org/work/W4296720204> ?p ?o ?g. }
- W4296720204 abstract "Abstract Glycyrrhiza uralensis Fisch. is prescribed as one of the original plants of licorice in Chinese Pharmacopoeia. This herbal medicine possesses numerous important pharmacological activities and has been used in clinic in China since ancient times. Glycyrrhizic acid (GA) is a triterpenoid compound isolated from G. uralensis and also one of the marker components for its quality control. Based on our pervious transcriptome study, three genes, the caffeic acid 3-O-methyltransferase gene (COMT), the β-carotene 3-hydroxylase gene (CRTZ), and the flavonoid 3'-monooxygenase gene (F3'H), were selected as our target genes due to a high correlation of their expression levels with GA biosynthesis. In this study, we investigated the regulatory effects of these genes on GA biosynthesis through gene editing and overexpression in G. uralensis hairy roots. We observed that neither knockout nor overexpression of any of the genes affects the viability of the transgenic hairy roots, indicating that these genes are not essential for survival of hairy roots. However, compared with the wild type and negative control hairy roots, GA contents were significantly lower in hairy roots overexpressing COMT, CRTZ, and F3'H, while higher in those knocking out the three genes. Our findings demonstrate that the three genes, COMT, CRTZ, and F3'H, all negatively regulate the GA biosynthesis." @default.
- W4296720204 created "2022-09-23" @default.
- W4296720204 creator A5008978381 @default.
- W4296720204 creator A5021901509 @default.
- W4296720204 creator A5031337173 @default.
- W4296720204 creator A5031897910 @default.
- W4296720204 creator A5042567653 @default.
- W4296720204 creator A5043920015 @default.
- W4296720204 creator A5090310655 @default.
- W4296720204 date "2022-09-21" @default.
- W4296720204 modified "2023-10-18" @default.
- W4296720204 title "COMT, CRTZ, and F3'H regulate glycyrrhizic acid biosynthesis in Glycyrrhizauralensis hairy roots" @default.
- W4296720204 cites W1830882552 @default.
- W4296720204 cites W1978121850 @default.
- W4296720204 cites W1996914966 @default.
- W4296720204 cites W1997064533 @default.
- W4296720204 cites W2004341139 @default.
- W4296720204 cites W2015270609 @default.
- W4296720204 cites W2016894948 @default.
- W4296720204 cites W2057829628 @default.
- W4296720204 cites W2092490637 @default.
- W4296720204 cites W2096801003 @default.
- W4296720204 cites W2098568749 @default.
- W4296720204 cites W2118677758 @default.
- W4296720204 cites W2163854702 @default.
- W4296720204 cites W2172122213 @default.
- W4296720204 cites W2300320800 @default.
- W4296720204 cites W2511171980 @default.
- W4296720204 cites W2593850994 @default.
- W4296720204 cites W2796907918 @default.
- W4296720204 cites W2801271512 @default.
- W4296720204 cites W2894745923 @default.
- W4296720204 cites W2895099230 @default.
- W4296720204 cites W2951372572 @default.
- W4296720204 cites W2952030297 @default.
- W4296720204 cites W2995101414 @default.
- W4296720204 cites W3011680545 @default.
- W4296720204 cites W3024136534 @default.
- W4296720204 cites W3034655519 @default.
- W4296720204 cites W3047017753 @default.
- W4296720204 cites W3095528145 @default.
- W4296720204 cites W3107979315 @default.
- W4296720204 cites W3132672653 @default.
- W4296720204 cites W3133888320 @default.
- W4296720204 cites W3135839881 @default.
- W4296720204 cites W3162435772 @default.
- W4296720204 cites W3164748257 @default.
- W4296720204 cites W3167916047 @default.
- W4296720204 cites W3178604706 @default.
- W4296720204 cites W3180194560 @default.
- W4296720204 cites W3183736492 @default.
- W4296720204 cites W3193243781 @default.
- W4296720204 cites W3200643060 @default.
- W4296720204 cites W3215730977 @default.
- W4296720204 cites W4200247069 @default.
- W4296720204 cites W4211232639 @default.
- W4296720204 cites W4211254291 @default.
- W4296720204 cites W4213338191 @default.
- W4296720204 doi "https://doi.org/10.21203/rs.3.rs-1509234/v3" @default.
- W4296720204 hasPublicationYear "2022" @default.
- W4296720204 type Work @default.
- W4296720204 citedByCount "0" @default.
- W4296720204 crossrefType "posted-content" @default.
- W4296720204 hasAuthorship W4296720204A5008978381 @default.
- W4296720204 hasAuthorship W4296720204A5021901509 @default.
- W4296720204 hasAuthorship W4296720204A5031337173 @default.
- W4296720204 hasAuthorship W4296720204A5031897910 @default.
- W4296720204 hasAuthorship W4296720204A5042567653 @default.
- W4296720204 hasAuthorship W4296720204A5043920015 @default.
- W4296720204 hasAuthorship W4296720204A5090310655 @default.
- W4296720204 hasBestOaLocation W42967202041 @default.
- W4296720204 hasConcept C102230213 @default.
- W4296720204 hasConcept C104317684 @default.
- W4296720204 hasConcept C122172344 @default.
- W4296720204 hasConcept C142724271 @default.
- W4296720204 hasConcept C150194340 @default.
- W4296720204 hasConcept C162317418 @default.
- W4296720204 hasConcept C204787440 @default.
- W4296720204 hasConcept C2779655422 @default.
- W4296720204 hasConcept C2780004758 @default.
- W4296720204 hasConcept C553450214 @default.
- W4296720204 hasConcept C55493867 @default.
- W4296720204 hasConcept C71924100 @default.
- W4296720204 hasConcept C86803240 @default.
- W4296720204 hasConceptScore W4296720204C102230213 @default.
- W4296720204 hasConceptScore W4296720204C104317684 @default.
- W4296720204 hasConceptScore W4296720204C122172344 @default.
- W4296720204 hasConceptScore W4296720204C142724271 @default.
- W4296720204 hasConceptScore W4296720204C150194340 @default.
- W4296720204 hasConceptScore W4296720204C162317418 @default.
- W4296720204 hasConceptScore W4296720204C204787440 @default.
- W4296720204 hasConceptScore W4296720204C2779655422 @default.
- W4296720204 hasConceptScore W4296720204C2780004758 @default.
- W4296720204 hasConceptScore W4296720204C553450214 @default.
- W4296720204 hasConceptScore W4296720204C55493867 @default.
- W4296720204 hasConceptScore W4296720204C71924100 @default.
- W4296720204 hasConceptScore W4296720204C86803240 @default.
- W4296720204 hasLocation W42967202041 @default.
- W4296720204 hasOpenAccess W4296720204 @default.
- W4296720204 hasPrimaryLocation W42967202041 @default.