FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4296744144> ?p ?o ?g. }

• W4296744144 abstract "Faithful DNA replication is essential for all life. A multi-protein complex called the replisome contains all the enzymatic activities required to facilitate DNA replication, including unwinding parental DNA and synthesizing two identical daughter molecules. Faithful DNA replication can be challenged by both intrinsic and extrinsic factors, which can result in roadblocks to replication, causing incomplete replication, genomic instability, and an increased mutational load. This increased mutational load can ultimately lead to a number of diseases, a notable example being cancer. A key example of a roadblock to replication is chemical modifications in the DNA caused by exposure to ultraviolet light. Protein dynamics are thought to play a crucial role to the molecular pathways that occur in the presence of such DNA lesions, including potential damage bypass. Therefore, many assays have been developed to study these dynamics. In this review, we discuss three methods that can be used to study protein dynamics during replisome–lesion encounters in replication reactions reconstituted from purified proteins. Specifically, we focus on ensemble biochemical assays, single-molecule fluorescence, and cryo-electron microscopy. We discuss two key model DNA replication systems, derived from Escherichia coli and Saccharomyces cerevisiae . The main methods of choice to study replication over the last decades have involved biochemical assays that rely on ensemble averaging. While these assays do not provide a direct readout of protein dynamics, they can often be inferred. More recently, single-molecule techniques including single-molecule fluorescence microscopy have been used to visualize replisomes encountering lesions in real time. In these experiments, individual proteins can be fluorescently labeled in order to observe the dynamics of specific proteins during DNA replication. Finally, cryo-electron microscopy can provide detailed structures of individual replisome components, which allows functional data to be interpreted in a structural context. While classic cryo-electron microscopy approaches provide static information, recent developments such as time-resolved cryo-electron microscopy help to bridge the gap between static structures and dynamic single-molecule techniques by visualizing sequential steps in biochemical pathways. In combination, these techniques will be capable of visualizing DNA replication and lesion encounter dynamics in real time, whilst observing the structural changes that facilitate these dynamics." @default.
• W4296744144 created "2022-09-23" @default.
• W4296744144 creator A5022505678 @default.
• W4296744144 creator A5039468934 @default.
• W4296744144 creator A5075591359 @default.
• W4296744144 date "2022-09-21" @default.
• W4296744144 modified "2023-10-18" @default.
• W4296744144 title "Observing protein dynamics during DNA-lesion bypass by the replisome" @default.
• W4296744144 cites W1492057161 @default.
• W4296744144 cites W1504519388 @default.
• W4296744144 cites W1538419182 @default.
• W4296744144 cites W1545241568 @default.
• W4296744144 cites W1548610738 @default.
• W4296744144 cites W1604458367 @default.
• W4296744144 cites W1606232948 @default.
• W4296744144 cites W1652360688 @default.
• W4296744144 cites W1964549900 @default.
• W4296744144 cites W1964668112 @default.
• W4296744144 cites W1968393019 @default.
• W4296744144 cites W1971658781 @default.
• W4296744144 cites W1975699037 @default.
• W4296744144 cites W1981148605 @default.
• W4296744144 cites W1984353960 @default.
• W4296744144 cites W1984743062 @default.
• W4296744144 cites W1985746590 @default.
• W4296744144 cites W1992082799 @default.
• W4296744144 cites W1995004577 @default.
• W4296744144 cites W1996109316 @default.
• W4296744144 cites W1999350701 @default.
• W4296744144 cites W2000826427 @default.
• W4296744144 cites W2013460204 @default.
• W4296744144 cites W2015471659 @default.
• W4296744144 cites W2023248068 @default.
• W4296744144 cites W2025007049 @default.
• W4296744144 cites W2027640710 @default.
• W4296744144 cites W2032004743 @default.
• W4296744144 cites W2033870603 @default.
• W4296744144 cites W2039861913 @default.
• W4296744144 cites W2052508338 @default.
• W4296744144 cites W2053693072 @default.
• W4296744144 cites W2057692066 @default.
• W4296744144 cites W2061676522 @default.
• W4296744144 cites W2065626377 @default.
• W4296744144 cites W2065793580 @default.
• W4296744144 cites W2066975479 @default.
• W4296744144 cites W2068853142 @default.
• W4296744144 cites W2070117236 @default.
• W4296744144 cites W2078670754 @default.
• W4296744144 cites W2083123225 @default.
• W4296744144 cites W2083158919 @default.
• W4296744144 cites W2085365618 @default.
• W4296744144 cites W2087406053 @default.
• W4296744144 cites W2094006139 @default.
• W4296744144 cites W2096963388 @default.
• W4296744144 cites W2101472561 @default.
• W4296744144 cites W2106285580 @default.
• W4296744144 cites W2106393979 @default.
• W4296744144 cites W2108539496 @default.
• W4296744144 cites W2110095772 @default.
• W4296744144 cites W2117847813 @default.
• W4296744144 cites W2124221060 @default.
• W4296744144 cites W2126915623 @default.
• W4296744144 cites W2129956410 @default.
• W4296744144 cites W2134153749 @default.
• W4296744144 cites W2138180167 @default.
• W4296744144 cites W2140962015 @default.
• W4296744144 cites W2141439330 @default.
• W4296744144 cites W2141512093 @default.
• W4296744144 cites W2141616726 @default.
• W4296744144 cites W2163965181 @default.
• W4296744144 cites W2164521560 @default.
• W4296744144 cites W2166150079 @default.
• W4296744144 cites W2194888552 @default.
• W4296744144 cites W2221345846 @default.
• W4296744144 cites W2239694049 @default.
• W4296744144 cites W2258280387 @default.
• W4296744144 cites W2305654697 @default.
• W4296744144 cites W2327611812 @default.
• W4296744144 cites W2410925476 @default.
• W4296744144 cites W2415913162 @default.
• W4296744144 cites W2468004024 @default.
• W4296744144 cites W2472184758 @default.
• W4296744144 cites W2473838984 @default.
• W4296744144 cites W2565335750 @default.
• W4296744144 cites W2568465283 @default.
• W4296744144 cites W2578571972 @default.
• W4296744144 cites W2600668326 @default.
• W4296744144 cites W2602490333 @default.
• W4296744144 cites W2605294494 @default.
• W4296744144 cites W2606131944 @default.
• W4296744144 cites W2612680646 @default.
• W4296744144 cites W2625757644 @default.
• W4296744144 cites W2711675708 @default.
• W4296744144 cites W2737463560 @default.
• W4296744144 cites W2740695644 @default.
• W4296744144 cites W2742880086 @default.
• W4296744144 cites W2762899632 @default.
• W4296744144 cites W2774792866 @default.
• W4296744144 cites W2779630731 @default.
• W4296744144 cites W2781499568 @default.

• next