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• W4296773192 abstract "Apoptotic vesicles (apoVs) are specific extracellular vesicles generated during apoptosis and play important roles in multiple physiological and pathophysiological settings. Here, we present a protocol using differential centrifugation to separate apoVs from human mesenchymal stem cells (MSCs) after induction of apoptosis. We describe how to characterize apoV size and morphology by nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM), and determination of specific biomarker expression by immunoblotting. Our protocol will be useful for preparing apoVs for further functional analysis. For complete details on the use and execution of this protocol, please refer to Zheng et al. (2021)." @default.
• W4296773192 created "2022-09-23" @default.
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• W4296773192 date "2022-12-01" @default.
• W4296773192 modified "2023-10-16" @default.
• W4296773192 title "Protocol for differential centrifugation-based separation and characterization of apoptotic vesicles derived from human mesenchymal stem cells" @default.
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• W4296773192 doi "https://doi.org/10.1016/j.xpro.2022.101695" @default.
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