FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4296781330> ?p ?o ?g. }

• W4296781330 abstract "Biochanin A (Bio-A), an isoflavone abundant in chickpeas, possesses hypoglycemic, hypolipidemic, and anti-inflammatory effects. However, whether Bio-A has antihepatosteatosis effect remains unclear. This study aimed to evaluate the antihepatosteatosis effect of Bio-A on oleate (OA)-treated hepatocytes, and explore the underlying mechanism. When incubated with OA for 24 h, HepG2 cells were treated with various concentrations of Bio-A for 24 h to obtain an optimal antihepatosteatosis dose. HepG2 cells were treated with the AMP-activated protein kinase (AMPK) inhibitor Compound C, or the sirtuin-3 (SIRT3) inhibitor 3-TYP, and incubated with 50 μM Bio-A. The results indicated that 12.6% of lipid content, particularly 11.0% of triglyceride content, and the expression of adipocyte differentiation-related protein were significantly decreased in Bio-A-treated hepatosteatosis cells, followed by an increase in the expression of Beclin 1, phosphorylation of Unc-51-like kinase 1 (ULK-1), the microtubule-associated protein 1 light chain 3 (LC3)-II/LC3-I ratio, and a decrease in expression of p62. The results indicated that Bio-A upregulated autophagosome formation and autophagy flux. In addition, Bio-A increased SIRT3 expression and AMPK phosphorylation in OA-treated HepG2 cells. Blockade of AMPK and SIRT3 blocked the antihepatosteatosis effect and ULK-1 activation by Bio-A. AMPK inhibition did not eliminate the activation of SIRT3 by Bio-A. AutoDock analysis demonstrated that interaction might exist between Bio-A and SIRT3. In conclusion, Bio-A reduced fat accumulation in OA-treated HepG2 cells by activating SIRT3/AMPK/ULK-1-mediated autophagy. The findings provide a theoretical basis for the effect of Bio-A on hepatic steatosis-related diseases. PRACTICAL APPLICATIONS: This study highlights the antihepatosteatosis effects of biochanin A (Bio-A) on oleate (OA)-treated hepatocytes. Bio-A, one of the isoflavones in Cicer arietinum Linn., possesses multiple bioactivities such as antiobesity, anti-inflammation, and hypoglycemic and hypolipidemic effects. This study provides a new application of Bio-A to treat hepatic steatosis, and revealed the underlying mechanism of Bio-A involved in the activation of the SIRT3/AMPK/ULK-1-mediated autophagy. The findings provide a theoretical basis for the application of Bio-A to hepatic steatosis-related diseases." @default.
• W4296781330 created "2022-09-23" @default.
• W4296781330 creator A5003672906 @default.
• W4296781330 creator A5011679458 @default.
• W4296781330 creator A5011706182 @default.
• W4296781330 creator A5013062951 @default.
• W4296781330 creator A5022167110 @default.
• W4296781330 creator A5068653286 @default.
• W4296781330 creator A5084152574 @default.
• W4296781330 date "2022-09-20" @default.
• W4296781330 modified "2023-09-26" @default.
• W4296781330 title "Biochanin A ameliorated oleate‐induced steatosis in HepG2 cells by activating the SIRT3/AMPK/ULK‐1 signaling pathway" @default.
• W4296781330 cites W1974094677 @default.
• W4296781330 cites W2017779665 @default.
• W4296781330 cites W2082740501 @default.
• W4296781330 cites W2210672989 @default.
• W4296781330 cites W2463809732 @default.
• W4296781330 cites W2531005628 @default.
• W4296781330 cites W2800229438 @default.
• W4296781330 cites W2800474870 @default.
• W4296781330 cites W2804270029 @default.
• W4296781330 cites W2889508929 @default.
• W4296781330 cites W2987022733 @default.
• W4296781330 cites W3000690558 @default.
• W4296781330 cites W3005032916 @default.
• W4296781330 cites W3013400299 @default.
• W4296781330 cites W3040704922 @default.
• W4296781330 cites W3106887263 @default.
• W4296781330 cites W3117050386 @default.
• W4296781330 cites W3119790932 @default.
• W4296781330 cites W3128373527 @default.
• W4296781330 cites W3157948315 @default.
• W4296781330 cites W3167208319 @default.
• W4296781330 cites W4224255146 @default.
• W4296781330 cites W4285732188 @default.
• W4296781330 cites W4286512894 @default.
• W4296781330 doi "https://doi.org/10.1111/jfbc.14428" @default.
• W4296781330 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36125796" @default.
• W4296781330 hasPublicationYear "2022" @default.
• W4296781330 type Work @default.
• W4296781330 citedByCount "2" @default.
• W4296781330 countsByYear W42967813302023 @default.
• W4296781330 crossrefType "journal-article" @default.
• W4296781330 hasAuthorship W4296781330A5003672906 @default.
• W4296781330 hasAuthorship W4296781330A5011679458 @default.
• W4296781330 hasAuthorship W4296781330A5011706182 @default.
• W4296781330 hasAuthorship W4296781330A5013062951 @default.
• W4296781330 hasAuthorship W4296781330A5022167110 @default.
• W4296781330 hasAuthorship W4296781330A5068653286 @default.
• W4296781330 hasAuthorship W4296781330A5084152574 @default.
• W4296781330 hasBestOaLocation W42967813301 @default.
• W4296781330 hasConcept C11960822 @default.
• W4296781330 hasConcept C134018914 @default.
• W4296781330 hasConcept C138488276 @default.
• W4296781330 hasConcept C181199279 @default.
• W4296781330 hasConcept C184235292 @default.
• W4296781330 hasConcept C185592680 @default.
• W4296781330 hasConcept C190283241 @default.
• W4296781330 hasConcept C203522944 @default.
• W4296781330 hasConcept C25095133 @default.
• W4296781330 hasConcept C2776175330 @default.
• W4296781330 hasConcept C2776780712 @default.
• W4296781330 hasConcept C2778298627 @default.
• W4296781330 hasConcept C2778821405 @default.
• W4296781330 hasConcept C2780124434 @default.
• W4296781330 hasConcept C2780561631 @default.
• W4296781330 hasConcept C2780955279 @default.
• W4296781330 hasConcept C55493867 @default.
• W4296781330 hasConcept C75520062 @default.
• W4296781330 hasConcept C86803240 @default.
• W4296781330 hasConcept C95444343 @default.
• W4296781330 hasConcept C97029542 @default.
• W4296781330 hasConceptScore W4296781330C11960822 @default.
• W4296781330 hasConceptScore W4296781330C134018914 @default.
• W4296781330 hasConceptScore W4296781330C138488276 @default.
• W4296781330 hasConceptScore W4296781330C181199279 @default.
• W4296781330 hasConceptScore W4296781330C184235292 @default.
• W4296781330 hasConceptScore W4296781330C185592680 @default.
• W4296781330 hasConceptScore W4296781330C190283241 @default.
• W4296781330 hasConceptScore W4296781330C203522944 @default.
• W4296781330 hasConceptScore W4296781330C25095133 @default.
• W4296781330 hasConceptScore W4296781330C2776175330 @default.
• W4296781330 hasConceptScore W4296781330C2776780712 @default.
• W4296781330 hasConceptScore W4296781330C2778298627 @default.
• W4296781330 hasConceptScore W4296781330C2778821405 @default.
• W4296781330 hasConceptScore W4296781330C2780124434 @default.
• W4296781330 hasConceptScore W4296781330C2780561631 @default.
• W4296781330 hasConceptScore W4296781330C2780955279 @default.
• W4296781330 hasConceptScore W4296781330C55493867 @default.
• W4296781330 hasConceptScore W4296781330C75520062 @default.
• W4296781330 hasConceptScore W4296781330C86803240 @default.
• W4296781330 hasConceptScore W4296781330C95444343 @default.
• W4296781330 hasConceptScore W4296781330C97029542 @default.
• W4296781330 hasFunder F4320321001 @default.
• W4296781330 hasIssue "12" @default.
• W4296781330 hasLocation W42967813301 @default.
• W4296781330 hasLocation W42967813302 @default.
• W4296781330 hasOpenAccess W4296781330 @default.
• W4296781330 hasPrimaryLocation W42967813301 @default.
• W4296781330 hasRelatedWork W2063678700 @default.

• next