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• W4296982355 abstract "The gastrointestinal (GI) tract, particularly the colon region, holds a highly diverse microbial community that plays an important role in the metabolism, physiology, nutrition, and immune function of the host body. Accumulating evidence has revealed that alteration in these microbial communities is the pivotal step in developing various metabolic diseases, including obesity, inflammatory bowel disease (IBD), and colorectal cancer. However, there is still a lack of clear understanding of the interrelationship between microbiota and diet as well as the effectiveness of chemoprevention strategies, including pre and probiotic agents in modifying the colonic microbiota and preventing digestive diseases. Existing methods for assessing these microbiota-diet interactions are often based on samples collected from the feces or endoscopy techniques which are incapable of providing information on spatial variations of the gut microbiota or are considered invasive procedures. To address this need, here we have developed an electronic-free smart capsule that enables site-specific sampling of the gut microbiome within the proximal colon region of the GI tract. The 3D printed device houses a superabsorbent hydrogel bonded onto a flexible polydimethylsiloxane (PDMS) disk that serves as a milieu to collect the fluid in the gut lumen and its microbiome by rapid swelling and providing the necessary mechanical actuation to close the capsule after the sampling is completed. The targeted colonic sampling is achieved by coating the sampling aperture on the capsule with a double-layer pH-sensitive enteric coating, which delays fluid in the lumen from entering the capsule until it reaches the proximal colon of the GI tract. To identify the appropriate pH-responsive double-layer coating and processing condition, a series of systematic dissolution characterizations in different pH conditions that mimicked the GI tract was conducted. The effective targeted microbial sampling performance and preservation of the smart capsule with the optimized design were validated using both realistic in vitro GI tract models with mixed bacteria cultures and in vivo with pigs as an animal model. The results from 16s rRNA and WideSeq analysis in both in vitro and in vivo studies showed that the bacterial population sampled within the retrieved capsule closely matched the bacterial population within the targeted sampling region (proximal colon). Herein, it is envisioned that such smart sampling capsule technology will provide new avenues for gastroenterological research and clinical applications, including diet-host-microbiome relationships, focused on human GI function and health. STATEMENT OF SIGNIFICANCE: The colonic microbiota plays a major role in the etiology of numerous diseases. Extensive efforts have been conducted to monitor the gut microbiome using sequencing technologies based on samples collected from feces or mucosal biopsies that are typically obtained by colonoscopy. Despite the simplicity of fecal sampling procedures, they are incapable of preserving spatial and temporal information about the bacteria through the gastrointestinal (GI) tract. In contrast, colonoscopy is an invasive and impractical approach to frequently assess the effect of dietary and therapeutic intake on the microbiome and their impact on the health of the patient. Here, we developed a non-invasive capsule that enables targeted sampling from the ascending colon, thereby providing crucial information for disease prediction and monitoring." @default.
• W4296982355 created "2022-09-25" @default.
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• W4296982355 date "2022-12-01" @default.
• W4296982355 modified "2023-10-11" @default.
• W4296982355 title "Smart capsule for targeted proximal colon microbiome sampling" @default.
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• W4296982355 cites W2010125549 @default.
• W4296982355 cites W2015567271 @default.
• W4296982355 cites W2035513563 @default.
• W4296982355 cites W2035984904 @default.
• W4296982355 cites W2048155800 @default.
• W4296982355 cites W2058362335 @default.
• W4296982355 cites W2082149095 @default.
• W4296982355 cites W2090925778 @default.
• W4296982355 cites W2097124003 @default.
• W4296982355 cites W2098642603 @default.
• W4296982355 cites W2113753061 @default.
• W4296982355 cites W2120495574 @default.
• W4296982355 cites W2128769815 @default.
• W4296982355 cites W2131186249 @default.
• W4296982355 cites W2139327060 @default.
• W4296982355 cites W2149848289 @default.
• W4296982355 cites W2169330513 @default.
• W4296982355 cites W2177822540 @default.
• W4296982355 cites W2328023807 @default.
• W4296982355 cites W2330435962 @default.
• W4296982355 cites W2343758687 @default.
• W4296982355 cites W2463118776 @default.
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• W4296982355 cites W2789006532 @default.
• W4296982355 cites W2888003279 @default.
• W4296982355 cites W2889977344 @default.
• W4296982355 cites W2901219667 @default.
• W4296982355 cites W2914668393 @default.
• W4296982355 cites W2920807146 @default.
• W4296982355 cites W2970045605 @default.
• W4296982355 cites W2978317856 @default.
• W4296982355 cites W2989675292 @default.
• W4296982355 cites W2997520701 @default.
• W4296982355 cites W3009569679 @default.
• W4296982355 cites W3009963727 @default.
• W4296982355 cites W3015992957 @default.
• W4296982355 cites W3018475212 @default.
• W4296982355 cites W3027118763 @default.
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• W4296982355 doi "https://doi.org/10.1016/j.actbio.2022.09.050" @default.
• W4296982355 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36162763" @default.
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