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- W4297140010 abstract "Detecting the simultaneous presence of a microRNA (miRNA) and a mRNA in a specific tissue can provide support for the prediction that the miRNA regulates the mRNA. Ｗe develop a method that uses sequence-specific miRNA-locked nucleic acid (LNA) and mRNA-LNA probes. Moreover, it augments the detection signal by rolling circle amplification, achieving a high signal-noise ratio at the single-cell level. Dot signals are counted for determining the expression levels of mRNA and miRNA molecules in specific cells. We show a high sequence specificity of our miRNA-LNA probe, revealing that it can discriminate single-base mismatches. Numerical quantification by our method is tested in transgenic rice lines with different gene expression levels." @default.
- W4297140010 created "2022-09-27" @default.
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- W4297140010 date "2022-08-27" @default.
- W4297140010 modified "2023-10-14" @default.
- W4297140010 title "Simultaneous detection of miRNA and mRNA at the single-cell level in plant tissues v1" @default.
- W4297140010 doi "https://doi.org/10.17504/protocols.io.x54v9deppg3e/v1" @default.
- W4297140010 hasPublicationYear "2022" @default.
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