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- W4297220220 abstract "The diversity and mosaic architecture of phage genomes present challenges for whole-genome phylogenies and comparative genomics. There are no universally conserved core genes, ∼70% of phage genes are of unknown function, and phage genomes are replete with small (<500 bp) open reading frames. Assembling sequence-related genes into phamilies (phams) based on amino acid sequence similarity simplifies comparative phage genomics and facilitates representations of phage genome mosaicism. With the rapid and substantial increase in the numbers of sequenced phage genomes, computationally efficient pham assembly is needed, together with strategies for including newly sequenced phage genomes. Here, we describe the Python package PhaMMseqs, which uses MMseqs2 for pham assembly, and we evaluate the key parameters for optimal pham assembly of sequence- and functionally related proteins. PhaMMseqs runs efficiently with only modest hardware requirements and integrates with the pdm_utils package for simple genome entry and export of datasets for evolutionary analyses and phage genome map construction." @default.
- W4297220220 created "2022-09-28" @default.
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- W4297220220 date "2022-09-26" @default.
- W4297220220 modified "2023-10-14" @default.
- W4297220220 title "PhaMMseqs: a new pipeline for constructing phage gene phamilies using MMseqs2" @default.
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- W4297220220 doi "https://doi.org/10.1093/g3journal/jkac233" @default.
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