Matches in SemOpenAlex for { <https://semopenalex.org/work/W4297238434> ?p ?o ?g. }
- W4297238434 abstract "Abstract Background Perturbation of DNA methyltransferases (DNMTs) and of the active DNA demethylation pathway via ten-eleven translocation (TET) methylcytosine dioxygenases results in severe developmental defects and embryonic lethality. Dynamic control of DNA methylation is therefore vital for embryogenesis, yet the underlying mechanisms remain poorly understood. Results Here we report a single-cell transcriptomic atlas from Dnmt and Tet mutant mouse embryos during early organogenesis. We show that both the maintenance and de novo methyltransferase enzymes are dispensable for the formation of all major cell types at E8.5. However, DNA methyltransferases are required for silencing of prior or alternative cell fates such as pluripotency and extraembryonic programmes. Deletion of all three TET enzymes produces substantial lineage biases, in particular, a failure to generate primitive erythrocytes. Single-cell multi-omics profiling moreover reveals that this is linked to a failure to demethylate distal regulatory elements in Tet triple-knockout embryos. Conclusions This study provides a detailed analysis of the effects of perturbing DNA methylation on mouse organogenesis at a whole organism scale and affords new insights into the regulatory mechanisms of cell fate decisions." @default.
- W4297238434 created "2022-09-28" @default.
- W4297238434 creator A5001445617 @default.
- W4297238434 creator A5005275806 @default.
- W4297238434 creator A5008565592 @default.
- W4297238434 creator A5017318024 @default.
- W4297238434 creator A5023056756 @default.
- W4297238434 creator A5042376614 @default.
- W4297238434 creator A5059416866 @default.
- W4297238434 creator A5066726255 @default.
- W4297238434 creator A5091359674 @default.
- W4297238434 date "2022-09-26" @default.
- W4297238434 modified "2023-10-17" @default.
- W4297238434 title "Single-cell multi-omics profiling links dynamic DNA methylation to cell fate decisions during mouse early organogenesis" @default.
- W4297238434 cites W1506411903 @default.
- W4297238434 cites W1528634627 @default.
- W4297238434 cites W1964941502 @default.
- W4297238434 cites W1965972811 @default.
- W4297238434 cites W1969642295 @default.
- W4297238434 cites W1997453048 @default.
- W4297238434 cites W2017163188 @default.
- W4297238434 cites W2018304597 @default.
- W4297238434 cites W2019552331 @default.
- W4297238434 cites W2021341670 @default.
- W4297238434 cites W2027097677 @default.
- W4297238434 cites W2029813631 @default.
- W4297238434 cites W2038449573 @default.
- W4297238434 cites W2040795628 @default.
- W4297238434 cites W2044282102 @default.
- W4297238434 cites W2046241907 @default.
- W4297238434 cites W2046812959 @default.
- W4297238434 cites W2053481440 @default.
- W4297238434 cites W2060446375 @default.
- W4297238434 cites W2071365780 @default.
- W4297238434 cites W2075520328 @default.
- W4297238434 cites W2088237277 @default.
- W4297238434 cites W2096391240 @default.
- W4297238434 cites W2108664872 @default.
- W4297238434 cites W2111041757 @default.
- W4297238434 cites W2124933556 @default.
- W4297238434 cites W2126053533 @default.
- W4297238434 cites W2129270269 @default.
- W4297238434 cites W2130199018 @default.
- W4297238434 cites W2131034808 @default.
- W4297238434 cites W2131374955 @default.
- W4297238434 cites W2138207763 @default.
- W4297238434 cites W2155870354 @default.
- W4297238434 cites W2157896232 @default.
- W4297238434 cites W2167010568 @default.
- W4297238434 cites W2179438025 @default.
- W4297238434 cites W2222000795 @default.
- W4297238434 cites W2290125147 @default.
- W4297238434 cites W2317132940 @default.
- W4297238434 cites W2407888728 @default.
- W4297238434 cites W2516455020 @default.
- W4297238434 cites W2532812479 @default.
- W4297238434 cites W2540833380 @default.
- W4297238434 cites W2754109996 @default.
- W4297238434 cites W2793965282 @default.
- W4297238434 cites W2794521141 @default.
- W4297238434 cites W2912272542 @default.
- W4297238434 cites W2951764674 @default.
- W4297238434 cites W2964849163 @default.
- W4297238434 cites W2992957227 @default.
- W4297238434 cites W2996429202 @default.
- W4297238434 cites W3012969293 @default.
- W4297238434 cites W3036426980 @default.
- W4297238434 cites W3045703643 @default.
- W4297238434 cites W3045805366 @default.
- W4297238434 cites W3111681797 @default.
- W4297238434 cites W3200646567 @default.
- W4297238434 cites W39364543 @default.
- W4297238434 cites W4244258249 @default.
- W4297238434 cites W4282925821 @default.
- W4297238434 cites W4297238434 @default.
- W4297238434 doi "https://doi.org/10.1186/s13059-022-02762-3" @default.
- W4297238434 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36163261" @default.
- W4297238434 hasPublicationYear "2022" @default.
- W4297238434 type Work @default.
- W4297238434 citedByCount "8" @default.
- W4297238434 countsByYear W42972384342022 @default.
- W4297238434 countsByYear W42972384342023 @default.
- W4297238434 crossrefType "journal-article" @default.
- W4297238434 hasAuthorship W4297238434A5001445617 @default.
- W4297238434 hasAuthorship W4297238434A5005275806 @default.
- W4297238434 hasAuthorship W4297238434A5008565592 @default.
- W4297238434 hasAuthorship W4297238434A5017318024 @default.
- W4297238434 hasAuthorship W4297238434A5023056756 @default.
- W4297238434 hasAuthorship W4297238434A5042376614 @default.
- W4297238434 hasAuthorship W4297238434A5059416866 @default.
- W4297238434 hasAuthorship W4297238434A5066726255 @default.
- W4297238434 hasAuthorship W4297238434A5091359674 @default.
- W4297238434 hasBestOaLocation W42972384341 @default.
- W4297238434 hasConcept C104317684 @default.
- W4297238434 hasConcept C145103041 @default.
- W4297238434 hasConcept C150194340 @default.
- W4297238434 hasConcept C162317418 @default.
- W4297238434 hasConcept C163952510 @default.
- W4297238434 hasConcept C16824249 @default.
- W4297238434 hasConcept C190727270 @default.