Matches in SemOpenAlex for { <https://semopenalex.org/work/W4297248826> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W4297248826 endingPage "383" @default.
- W4297248826 startingPage "377" @default.
- W4297248826 abstract "Abstract Ferredoxin-NADP+ reductase (FNR) in plants receives electrons from ferredoxin (Fd) and converts NADP+ to NADPH at the end of the photosynthetic electron transfer chain. We previously showed that the interaction between FNR and Fd was weakened by the allosteric binding of NADP(H) on FNR, which was considered as a part of negative cooperativity. In this study, we investigated the molecular mechanism of this phenomenon using maize (Zea mays L.) FNR and Fd, as the 3D structure of this Fd:FNR complex is available. Site-specific mutants of several amino acid residues on the Fd:FNR interface were analysed for the effect on the negative cooperativity, by kinetic analysis of Fd:FNR electron transfer activity and by Fd-affinity chromatography. Mutations of Fd Arg40Gln and FNR Glu154Gln that disrupt one of the salt bridges in the Fd:FNR complex suppressed the negative cooperativity, indicating the involvement of the ion pair of Fd Arg40 and FNR Glu154 in the mechanism of the negative cooperativity. Unexpectedly, either mutation of Fd Arg40Gln or FNR Glu154Gln tends to increase the affinity between Fd and FNR, suggesting the role of this ion pair in the regulation of the Fd:FNR affinity by NADPH, rather than the stabilization of the Fd:FNR complex." @default.
- W4297248826 created "2022-09-28" @default.
- W4297248826 creator A5008770150 @default.
- W4297248826 creator A5028002349 @default.
- W4297248826 creator A5037226893 @default.
- W4297248826 date "2022-09-26" @default.
- W4297248826 modified "2023-10-01" @default.
- W4297248826 title "Molecular mechanism of negative cooperativity of ferredoxin-NADP <b>+</b> reductase by ferredoxin and NADP(H): role of the ion pair of ferredoxin Arg40 of and FNR Glu154" @default.
- W4297248826 cites W1505834253 @default.
- W4297248826 cites W1514245212 @default.
- W4297248826 cites W1903986304 @default.
- W4297248826 cites W1964600118 @default.
- W4297248826 cites W1984106119 @default.
- W4297248826 cites W1997132664 @default.
- W4297248826 cites W2014091118 @default.
- W4297248826 cites W2033467050 @default.
- W4297248826 cites W2068371198 @default.
- W4297248826 cites W2080006609 @default.
- W4297248826 cites W2112110544 @default.
- W4297248826 cites W2154934782 @default.
- W4297248826 cites W2162023899 @default.
- W4297248826 cites W2573340705 @default.
- W4297248826 cites W2602553099 @default.
- W4297248826 cites W2982633177 @default.
- W4297248826 cites W3124023821 @default.
- W4297248826 cites W4210863507 @default.
- W4297248826 doi "https://doi.org/10.1093/jb/mvac075" @default.
- W4297248826 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36162819" @default.
- W4297248826 hasPublicationYear "2022" @default.
- W4297248826 type Work @default.
- W4297248826 citedByCount "2" @default.
- W4297248826 countsByYear W42972488262022 @default.
- W4297248826 countsByYear W42972488262023 @default.
- W4297248826 crossrefType "journal-article" @default.
- W4297248826 hasAuthorship W4297248826A5008770150 @default.
- W4297248826 hasAuthorship W4297248826A5028002349 @default.
- W4297248826 hasAuthorship W4297248826A5037226893 @default.
- W4297248826 hasConcept C104317684 @default.
- W4297248826 hasConcept C107824862 @default.
- W4297248826 hasConcept C123669783 @default.
- W4297248826 hasConcept C134621786 @default.
- W4297248826 hasConcept C134651460 @default.
- W4297248826 hasConcept C143065580 @default.
- W4297248826 hasConcept C166014724 @default.
- W4297248826 hasConcept C166342909 @default.
- W4297248826 hasConcept C181199279 @default.
- W4297248826 hasConcept C185592680 @default.
- W4297248826 hasConcept C187250156 @default.
- W4297248826 hasConcept C2776961318 @default.
- W4297248826 hasConcept C55493867 @default.
- W4297248826 hasConcept C71240020 @default.
- W4297248826 hasConcept C75473681 @default.
- W4297248826 hasConcept C8010536 @default.
- W4297248826 hasConceptScore W4297248826C104317684 @default.
- W4297248826 hasConceptScore W4297248826C107824862 @default.
- W4297248826 hasConceptScore W4297248826C123669783 @default.
- W4297248826 hasConceptScore W4297248826C134621786 @default.
- W4297248826 hasConceptScore W4297248826C134651460 @default.
- W4297248826 hasConceptScore W4297248826C143065580 @default.
- W4297248826 hasConceptScore W4297248826C166014724 @default.
- W4297248826 hasConceptScore W4297248826C166342909 @default.
- W4297248826 hasConceptScore W4297248826C181199279 @default.
- W4297248826 hasConceptScore W4297248826C185592680 @default.
- W4297248826 hasConceptScore W4297248826C187250156 @default.
- W4297248826 hasConceptScore W4297248826C2776961318 @default.
- W4297248826 hasConceptScore W4297248826C55493867 @default.
- W4297248826 hasConceptScore W4297248826C71240020 @default.
- W4297248826 hasConceptScore W4297248826C75473681 @default.
- W4297248826 hasConceptScore W4297248826C8010536 @default.
- W4297248826 hasIssue "6" @default.
- W4297248826 hasLocation W42972488261 @default.
- W4297248826 hasLocation W42972488262 @default.
- W4297248826 hasOpenAccess W4297248826 @default.
- W4297248826 hasPrimaryLocation W42972488261 @default.
- W4297248826 hasRelatedWork W1479863189 @default.
- W4297248826 hasRelatedWork W1632087640 @default.
- W4297248826 hasRelatedWork W204916659 @default.
- W4297248826 hasRelatedWork W2068484152 @default.
- W4297248826 hasRelatedWork W2080479902 @default.
- W4297248826 hasRelatedWork W2111260018 @default.
- W4297248826 hasRelatedWork W2982633177 @default.
- W4297248826 hasRelatedWork W3124023821 @default.
- W4297248826 hasRelatedWork W4210863507 @default.
- W4297248826 hasRelatedWork W4297248826 @default.
- W4297248826 hasVolume "172" @default.
- W4297248826 isParatext "false" @default.
- W4297248826 isRetracted "false" @default.
- W4297248826 workType "article" @default.