FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4297250650> ?p ?o ?g. }

• W4297250650 endingPage "e14009" @default.
• W4297250650 startingPage "e14009" @default.
• W4297250650 abstract "Background Fasting has been widely used to improve various metabolic diseases in humans. Adaptive fasting is necessary for metabolic adaptation during prolonged fasting, which could overcome the great advantages of short-term fasting. The liver is the main organ responsible for energy metabolism and metabolic homeostasis. To date, we lack literature that describes the physiologically relevant adaptations of the liver during prolonged fasting and refeeding. For that reason, this study aims to evaluate the response of the liver of Sprague-Dawley (SD) rats to prolonged fasting and refeeding. Methods Sixty-six male SD rats were divided into the fasting groups, which were fasted for 0, 4, 8, 12, 24, 48, 72, or 96 h, and the refeeding groups, which were refed for 1, 3, or 6 days after 96 h of fasting. Serum glucose, TG, FFA, β -hydroxybutyrate, insulin, glucagon, leptin, adiponectin and FGF21 levels were assessed. The glucose content, PEPCK activity, TG concentration and FFA content were measured in liver tissue, and the expression of genes involved in gluconeogenesis (PEPCK and G6Pase), ketogenesis (PPAR α , CPT-1a and HMGCS2) and the protein expression of nutrient-sensing signaling molecules (AMPK, mTOR and SIRT1) were determined by RT-qPCR and western blotting, respectively. Results Fasting significantly decreased the body weight, which was totally recovered to baseline after 3 days of refeeding. A 4-day fast triggered an energy metabolic substrate shift from glucose to ketones and caused serum hormone changes and changes in the protein expression levels of nutrient-sensing signaling molecules. Glycogenolysis served as the primary fuel source during the first 24 h of fasting, while gluconeogenesis supplied the most glucose thereafter. Serum FFA concentrations increased significantly with 48 h of fasting. Serum FFAs partly caused high serum β -hydroxybutyrate levels, which became an important energy source with the prolongation of the fasting duration. One day of refeeding quickly reversed the energy substrate switch. Nutrient-sensing signaling molecules (AMPK and SIRT1 but not mTOR signaling) were highly expressed at the beginning of fasting (in the first 4 h). Serum insulin and leptin decreased with fasting initiation, and serum glucagon increased, but adiponectin and FGF21 showed no significant changes. Herein, we depicted in detail the timing of the metabolic response and adaptation of the liver to a 4-day water-only fast and subsequent refeeding in rats, which provides helpful support for the design of safe prolonged and intermittent fasting regimens." @default.
• W4297250650 created "2022-09-28" @default.
• W4297250650 creator A5004046059 @default.
• W4297250650 creator A5022979781 @default.
• W4297250650 creator A5027590617 @default.
• W4297250650 creator A5027748236 @default.
• W4297250650 creator A5027817973 @default.
• W4297250650 creator A5031069096 @default.
• W4297250650 creator A5054126873 @default.
• W4297250650 creator A5058691896 @default.
• W4297250650 creator A5074543316 @default.
• W4297250650 creator A5075980805 @default.
• W4297250650 creator A5078942546 @default.
• W4297250650 creator A5079819931 @default.
• W4297250650 date "2022-09-20" @default.
• W4297250650 modified "2023-09-23" @default.
• W4297250650 title "Hepatic metabolite responses to 4-day complete fasting and subsequent refeeding in rats" @default.
• W4297250650 cites W1502152603 @default.
• W4297250650 cites W1503760388 @default.
• W4297250650 cites W1505404389 @default.
• W4297250650 cites W1595528489 @default.
• W4297250650 cites W1758135763 @default.
• W4297250650 cites W182302374 @default.
• W4297250650 cites W1906171613 @default.
• W4297250650 cites W1964378492 @default.
• W4297250650 cites W1971155457 @default.
• W4297250650 cites W1972762474 @default.
• W4297250650 cites W1974802955 @default.
• W4297250650 cites W1979957210 @default.
• W4297250650 cites W1983394635 @default.
• W4297250650 cites W2006936501 @default.
• W4297250650 cites W2013438463 @default.
• W4297250650 cites W2026433816 @default.
• W4297250650 cites W2034800706 @default.
• W4297250650 cites W2053965920 @default.
• W4297250650 cites W2065220901 @default.
• W4297250650 cites W2073212868 @default.
• W4297250650 cites W2098469794 @default.
• W4297250650 cites W2100308237 @default.
• W4297250650 cites W2106745402 @default.
• W4297250650 cites W2106867120 @default.
• W4297250650 cites W2107277218 @default.
• W4297250650 cites W2113530540 @default.
• W4297250650 cites W2119331212 @default.
• W4297250650 cites W2129132663 @default.
• W4297250650 cites W2158805869 @default.
• W4297250650 cites W2160151404 @default.
• W4297250650 cites W2182943525 @default.
• W4297250650 cites W2192080449 @default.
• W4297250650 cites W2290893040 @default.
• W4297250650 cites W2326032181 @default.
• W4297250650 cites W2346478374 @default.
• W4297250650 cites W2527481631 @default.
• W4297250650 cites W2528674304 @default.
• W4297250650 cites W2592437910 @default.
• W4297250650 cites W26309257 @default.
• W4297250650 cites W2736390409 @default.
• W4297250650 cites W2747163024 @default.
• W4297250650 cites W2753709051 @default.
• W4297250650 cites W2772607655 @default.
• W4297250650 cites W2785943060 @default.
• W4297250650 cites W2792489747 @default.
• W4297250650 cites W2801420986 @default.
• W4297250650 cites W2886742762 @default.
• W4297250650 cites W2888029645 @default.
• W4297250650 cites W2904373968 @default.
• W4297250650 cites W2913319475 @default.
• W4297250650 cites W2921788949 @default.
• W4297250650 cites W2941227083 @default.
• W4297250650 cites W2945838526 @default.
• W4297250650 cites W2970161534 @default.
• W4297250650 cites W2985685301 @default.
• W4297250650 cites W3004342319 @default.
• W4297250650 cites W3009104512 @default.
• W4297250650 cites W3017090181 @default.
• W4297250650 cites W3063362198 @default.
• W4297250650 cites W3083050368 @default.
• W4297250650 cites W3092947924 @default.
• W4297250650 cites W3096443278 @default.
• W4297250650 cites W3111965142 @default.
• W4297250650 cites W3113137941 @default.
• W4297250650 cites W3118955204 @default.
• W4297250650 cites W3140447913 @default.
• W4297250650 cites W3200046301 @default.
• W4297250650 cites W4205682743 @default.
• W4297250650 cites W4223445396 @default.
• W4297250650 cites W4247878364 @default.
• W4297250650 cites W2071530142 @default.
• W4297250650 doi "https://doi.org/10.7717/peerj.14009" @default.
• W4297250650 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36157064" @default.
• W4297250650 hasPublicationYear "2022" @default.
• W4297250650 type Work @default.
• W4297250650 citedByCount "1" @default.
• W4297250650 countsByYear W42972506502023 @default.
• W4297250650 crossrefType "journal-article" @default.
• W4297250650 hasAuthorship W4297250650A5004046059 @default.
• W4297250650 hasAuthorship W4297250650A5022979781 @default.
• W4297250650 hasAuthorship W4297250650A5027590617 @default.

• next