FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4297264967> ?p ?o ?g. }

• W4297264967 endingPage "e1010573" @default.
• W4297264967 startingPage "e1010573" @default.
• W4297264967 abstract "Fluorescence Recovery After Photobleaching (FRAP) has been extensively used to understand molecular dynamics in cells. This technique when applied to soluble, globular molecules driven by diffusion is easily interpreted and well understood. However, the classical methods of analysis cannot be applied to anisotropic structures subjected to directed transport, such as cytoskeletal filaments or elongated organelles transported along microtubule tracks. A new mathematical approach is needed to analyze FRAP data in this context and determine what information can be obtain from such experiments. To address these questions, we analyze fluorescence intensity profile curves after photobleaching of fluorescently labelled intermediate filaments anterogradely transported along microtubules. We apply the analysis to intermediate filament data to determine information about the filament motion. Our analysis consists of deriving equations for fluorescence intensity profiles and developing a mathematical model for the motion of filaments and simulating the model. Two closed forms for profile curves were derived, one for filaments of constant length and one for filaments with constant velocity, and three types of simulation were carried out. In the first type of simulation, the filaments have random velocities which are constant for the duration of the simulation. In the second type, filaments have random velocities which instantaneously change at random times. In the third type, filaments have random velocities and exhibit pausing between velocity changes. Our analysis shows: the most important distribution governing the shape of the intensity profile curves obtained from filaments is the distribution of the filament velocity. Furthermore, filament length which is constant during the experiment, had little impact on intensity profile curves. Finally, gamma distributions for the filament velocity with pauses give the best fit to asymmetric fluorescence intensity profiles of intermediate filaments observed in FRAP experiments performed in polarized migrating astrocytes. Our analysis also shows that the majority of filaments are stationary. Overall, our data give new insight into the regulation of intermediate filament dynamics during cell migration." @default.
• W4297264967 created "2022-09-28" @default.
• W4297264967 creator A5038506027 @default.
• W4297264967 creator A5042150923 @default.
• W4297264967 creator A5063255056 @default.
• W4297264967 creator A5075241789 @default.
• W4297264967 creator A5079062349 @default.
• W4297264967 creator A5081410860 @default.
• W4297264967 date "2022-09-26" @default.
• W4297264967 modified "2023-10-17" @default.
• W4297264967 title "Using Fluorescence Recovery After Photobleaching data to uncover filament dynamics" @default.
• W4297264967 cites W132683945 @default.
• W4297264967 cites W1965917412 @default.
• W4297264967 cites W1976676780 @default.
• W4297264967 cites W1998399559 @default.
• W4297264967 cites W2008679290 @default.
• W4297264967 cites W2032261838 @default.
• W4297264967 cites W2032300143 @default.
• W4297264967 cites W2034844603 @default.
• W4297264967 cites W2056986410 @default.
• W4297264967 cites W2066069779 @default.
• W4297264967 cites W2076328566 @default.
• W4297264967 cites W2078056153 @default.
• W4297264967 cites W2080544485 @default.
• W4297264967 cites W2083949600 @default.
• W4297264967 cites W2085256553 @default.
• W4297264967 cites W2089191944 @default.
• W4297264967 cites W2092619797 @default.
• W4297264967 cites W2123801722 @default.
• W4297264967 cites W2153436393 @default.
• W4297264967 cites W2157308869 @default.
• W4297264967 cites W2338001779 @default.
• W4297264967 cites W2444321040 @default.
• W4297264967 cites W2607357221 @default.
• W4297264967 cites W2608945653 @default.
• W4297264967 cites W278377992 @default.
• W4297264967 cites W2885936857 @default.
• W4297264967 cites W2886669587 @default.
• W4297264967 cites W2907679687 @default.
• W4297264967 cites W2941594620 @default.
• W4297264967 cites W3110101652 @default.
• W4297264967 cites W4282028942 @default.
• W4297264967 doi "https://doi.org/10.1371/journal.pcbi.1010573" @default.
• W4297264967 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36156590" @default.
• W4297264967 hasPublicationYear "2022" @default.
• W4297264967 type Work @default.
• W4297264967 citedByCount "1" @default.
• W4297264967 countsByYear W42972649672023 @default.
• W4297264967 crossrefType "journal-article" @default.
• W4297264967 hasAuthorship W4297264967A5038506027 @default.
• W4297264967 hasAuthorship W4297264967A5042150923 @default.
• W4297264967 hasAuthorship W4297264967A5063255056 @default.
• W4297264967 hasAuthorship W4297264967A5075241789 @default.
• W4297264967 hasAuthorship W4297264967A5079062349 @default.
• W4297264967 hasAuthorship W4297264967A5081410860 @default.
• W4297264967 hasBestOaLocation W42972649671 @default.
• W4297264967 hasConcept C120665830 @default.
• W4297264967 hasConcept C121332964 @default.
• W4297264967 hasConcept C127313418 @default.
• W4297264967 hasConcept C14228908 @default.
• W4297264967 hasConcept C142669718 @default.
• W4297264967 hasConcept C1491633281 @default.
• W4297264967 hasConcept C151730666 @default.
• W4297264967 hasConcept C185592680 @default.
• W4297264967 hasConcept C186060115 @default.
• W4297264967 hasConcept C199360897 @default.
• W4297264967 hasConcept C20418707 @default.
• W4297264967 hasConcept C26659971 @default.
• W4297264967 hasConcept C2777027219 @default.
• W4297264967 hasConcept C2779343474 @default.
• W4297264967 hasConcept C31332276 @default.
• W4297264967 hasConcept C41008148 @default.
• W4297264967 hasConcept C53403541 @default.
• W4297264967 hasConcept C55493867 @default.
• W4297264967 hasConcept C69357855 @default.
• W4297264967 hasConcept C86803240 @default.
• W4297264967 hasConcept C91881484 @default.
• W4297264967 hasConcept C95444343 @default.
• W4297264967 hasConcept C97355855 @default.
• W4297264967 hasConceptScore W4297264967C120665830 @default.
• W4297264967 hasConceptScore W4297264967C121332964 @default.
• W4297264967 hasConceptScore W4297264967C127313418 @default.
• W4297264967 hasConceptScore W4297264967C14228908 @default.
• W4297264967 hasConceptScore W4297264967C142669718 @default.
• W4297264967 hasConceptScore W4297264967C1491633281 @default.
• W4297264967 hasConceptScore W4297264967C151730666 @default.
• W4297264967 hasConceptScore W4297264967C185592680 @default.
• W4297264967 hasConceptScore W4297264967C186060115 @default.
• W4297264967 hasConceptScore W4297264967C199360897 @default.
• W4297264967 hasConceptScore W4297264967C20418707 @default.
• W4297264967 hasConceptScore W4297264967C26659971 @default.
• W4297264967 hasConceptScore W4297264967C2777027219 @default.
• W4297264967 hasConceptScore W4297264967C2779343474 @default.
• W4297264967 hasConceptScore W4297264967C31332276 @default.
• W4297264967 hasConceptScore W4297264967C41008148 @default.
• W4297264967 hasConceptScore W4297264967C53403541 @default.
• W4297264967 hasConceptScore W4297264967C55493867 @default.
• W4297264967 hasConceptScore W4297264967C69357855 @default.

• next