Matches in SemOpenAlex for { <https://semopenalex.org/work/W4297265418> ?p ?o ?g. }
- W4297265418 abstract "Phosphoinositides are pivotal regulators of vesicular traffic and signaling during phagocytosis. Phagosome formation, the initial step of the process, is characterized by local membrane remodeling and reorganization of the actin cytoskeleton that leads to formation of the pseudopods that drive particle engulfment. Using genetically encoded fluorescent probes, we found that upon particle engagement a localized pool of PtdIns(3,4)P2 is generated by the sequential activities of class I phosphoinositide 3-kinases and phosphoinositide 5-phosphatases. Depletion of this locally generated pool of PtdIns(3,4)P2 blocks pseudopod progression and ultimately phagocytosis. We show that the PtdIns(3,4)P2 effector Lamellipodin (Lpd) is recruited to nascent phagosomes by PtdIns(3,4)P2. Furthermore, we show that silencing of Lpd inhibits phagocytosis and produces aberrant pseudopodia with disorganized actin filaments. Finally, vasodilator-stimulated phosphoprotein (VASP) was identified as a key actin-regulatory protein mediating phagosome formation downstream of Lpd. Mechanistically, our findings imply that a pathway involving PtdIns(3,4)P2, Lpd, and VASP mediates phagocytosis at the stage of particle engulfment." @default.
- W4297265418 created "2022-09-28" @default.
- W4297265418 creator A5029793456 @default.
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- W4297265418 date "2022-09-27" @default.
- W4297265418 modified "2023-10-17" @default.
- W4297265418 title "PtdIns(3,4)P2, Lamellipodin, and VASP coordinate actin dynamics during phagocytosis in macrophages" @default.
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- W4297265418 cites W1895473399 @default.
- W4297265418 cites W1971766102 @default.
- W4297265418 cites W1973649882 @default.
- W4297265418 cites W1987338177 @default.
- W4297265418 cites W1991898819 @default.
- W4297265418 cites W1994586243 @default.
- W4297265418 cites W1999644120 @default.
- W4297265418 cites W2001023704 @default.
- W4297265418 cites W2005718392 @default.
- W4297265418 cites W2007658022 @default.
- W4297265418 cites W2007869203 @default.
- W4297265418 cites W2012116166 @default.
- W4297265418 cites W2012470118 @default.
- W4297265418 cites W2022060662 @default.
- W4297265418 cites W2024296170 @default.
- W4297265418 cites W2026393363 @default.
- W4297265418 cites W2027816974 @default.
- W4297265418 cites W2036882237 @default.
- W4297265418 cites W2059716736 @default.
- W4297265418 cites W2063693187 @default.
- W4297265418 cites W2069998843 @default.
- W4297265418 cites W2071442472 @default.
- W4297265418 cites W2091701502 @default.
- W4297265418 cites W2095878168 @default.
- W4297265418 cites W2096968286 @default.
- W4297265418 cites W2099809076 @default.
- W4297265418 cites W2104467943 @default.
- W4297265418 cites W2104603012 @default.
- W4297265418 cites W2112048948 @default.
- W4297265418 cites W2112305018 @default.
- W4297265418 cites W2115601768 @default.
- W4297265418 cites W2117835384 @default.
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- W4297265418 cites W2125335599 @default.
- W4297265418 cites W2136230579 @default.
- W4297265418 cites W2137629224 @default.
- W4297265418 cites W2139969808 @default.
- W4297265418 cites W2140804868 @default.
- W4297265418 cites W2144203350 @default.
- W4297265418 cites W2145676008 @default.
- W4297265418 cites W2159584083 @default.
- W4297265418 cites W2164614067 @default.
- W4297265418 cites W2165130769 @default.
- W4297265418 cites W2165448884 @default.
- W4297265418 cites W2167630566 @default.
- W4297265418 cites W2168539398 @default.
- W4297265418 cites W2231430019 @default.
- W4297265418 cites W2266150212 @default.
- W4297265418 cites W2272040821 @default.
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- W4297265418 cites W2320777303 @default.
- W4297265418 cites W2508382751 @default.
- W4297265418 cites W2547720710 @default.
- W4297265418 cites W2575051636 @default.
- W4297265418 cites W2584071154 @default.
- W4297265418 cites W2596080911 @default.
- W4297265418 cites W2613415318 @default.
- W4297265418 cites W2750137696 @default.
- W4297265418 cites W2765438719 @default.
- W4297265418 cites W2804278610 @default.
- W4297265418 cites W2883819410 @default.
- W4297265418 cites W2888800216 @default.
- W4297265418 cites W2908359717 @default.
- W4297265418 cites W2973308747 @default.
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- W4297265418 cites W3037074917 @default.
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- W4297265418 doi "https://doi.org/10.1083/jcb.202207042" @default.
- W4297265418 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36165850" @default.
- W4297265418 hasPublicationYear "2022" @default.
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