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- W4297387557 abstract "Live-cell imaging reveals the phenotypes and mechanisms of cellular function and their dysfunction that underscore cell physiology, development, and pathology. Here, we report a 3D super-resolution live-cell microscopy method by integrating radiality analysis and Fourier light-field microscopy ( rad- FLFM). We demonstrated the method using various live-cell specimens, including actins in Hela cells, microtubules in mammary organoid cells, and peroxisomes in COS-7 cells. Compared with conventional wide-field microscopy, rad- FLFM realizes scanning-free, volumetric 3D live-cell imaging with sub-diffraction-limited resolution of ∼150 nm ( x-y ) and 300 nm ( z ), milliseconds volume acquisition time, six-fold extended depth of focus of ∼6 µm, and low photodamage. The method provides a promising avenue to explore spatiotemporal-challenging subcellular processes in a wide range of cell biological research." @default.
- W4297387557 created "2022-09-28" @default.
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- W4297387557 date "2022-10-03" @default.
- W4297387557 modified "2023-10-14" @default.
- W4297387557 title "3D super-resolution live-cell imaging with radial symmetry and Fourier light-field microscopy" @default.
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- W4297387557 doi "https://doi.org/10.1364/boe.471967" @default.
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