FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4297460343> ?p ?o ?g. }

• W4297460343 endingPage "1376" @default.
• W4297460343 startingPage "1365" @default.
• W4297460343 abstract "The cereal cyst nematodes Heterodera avenae and Heterodera filipjevi are recognized as cyst nematodes that infect cereal crops and cause severe economic losses worldwide. Rapid, visual detection of cyst nematodes is essential for more effective control of this pest. In this study, recombinase polymerase amplification (RPA) combined with clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a (formerly known as cpf1) was developed for the rapid detection of H. avenae and H. filipjevi from infested field samples. The RPA reaction was performed at a wide range of temperatures from 35 to 42°C within 15 min. There was no cross-reactivity between H. avenae, H. filipjevi, and the common closely related plant-parasitic nematodes, indicating the high specificity of this assay. The detection limit of RPA-Cas12a was as low as 10 −4 single second-stage juvenile (J2), 10 −5 single cyst, and 0.001 ng of genomic DNA, which is 10 times greater than that of RPA-lateral flow dipstick (LFD) detection. The RPA-Cas12a assay was able to detect 10 −1 single J2 of H. avenae and H. filipjevi in 10 g of soil. In addition, the RPA-LFD assay and RPA-Cas12a assays could both quickly detect H. avenae and H. filipjevi from naturally infested soil, and the entire detection process could be completed within 1 h. These results indicated that the RPA-Cas12a assay developed herein is a simple, rapid, specific, sensitive, and visual method that can be easily adapted for the quick detection of H. avenae and H. filipjevi in infested fields." @default.
• W4297460343 created "2022-09-29" @default.
• W4297460343 creator A5006760859 @default.
• W4297460343 creator A5016299379 @default.
• W4297460343 creator A5038787515 @default.
• W4297460343 creator A5058287391 @default.
• W4297460343 creator A5070705794 @default.
• W4297460343 creator A5072172552 @default.
• W4297460343 creator A5074342094 @default.
• W4297460343 creator A5085226903 @default.
• W4297460343 creator A5086600889 @default.
• W4297460343 date "2023-05-01" @default.
• W4297460343 modified "2023-10-17" @default.
• W4297460343 title "Recombinase Polymerase Amplification Coupled with CRISPR-Cas12a Technology for Rapid and Highly Sensitive Detection of <i>Heterodera avenae</i> and <i>Heterodera filipjevi</i>" @default.
• W4297460343 cites W1574687937 @default.
• W4297460343 cites W1776353329 @default.
• W4297460343 cites W1967369504 @default.
• W4297460343 cites W1971426853 @default.
• W4297460343 cites W1983579253 @default.
• W4297460343 cites W1985464623 @default.
• W4297460343 cites W1985666890 @default.
• W4297460343 cites W1988566013 @default.
• W4297460343 cites W1989121690 @default.
• W4297460343 cites W2017060409 @default.
• W4297460343 cites W2030925316 @default.
• W4297460343 cites W2031287911 @default.
• W4297460343 cites W2034083046 @default.
• W4297460343 cites W2043375086 @default.
• W4297460343 cites W2049124377 @default.
• W4297460343 cites W2054594680 @default.
• W4297460343 cites W2066445818 @default.
• W4297460343 cites W2083370008 @default.
• W4297460343 cites W2116023979 @default.
• W4297460343 cites W2153350094 @default.
• W4297460343 cites W2165869860 @default.
• W4297460343 cites W2166910617 @default.
• W4297460343 cites W2170466485 @default.
• W4297460343 cites W2191378776 @default.
• W4297460343 cites W2341405505 @default.
• W4297460343 cites W2428784012 @default.
• W4297460343 cites W2592320452 @default.
• W4297460343 cites W2601387290 @default.
• W4297460343 cites W2604951316 @default.
• W4297460343 cites W2763859811 @default.
• W4297460343 cites W2766989075 @default.
• W4297460343 cites W2787024379 @default.
• W4297460343 cites W2801783434 @default.
• W4297460343 cites W2884217300 @default.
• W4297460343 cites W2885944176 @default.
• W4297460343 cites W2897741066 @default.
• W4297460343 cites W2910673378 @default.
• W4297460343 cites W2972894181 @default.
• W4297460343 cites W2977528794 @default.
• W4297460343 cites W2999920696 @default.
• W4297460343 cites W3017334101 @default.
• W4297460343 cites W3042243693 @default.
• W4297460343 cites W3081392899 @default.
• W4297460343 cites W3083789112 @default.
• W4297460343 cites W3107140780 @default.
• W4297460343 cites W3111827303 @default.
• W4297460343 cites W3125775467 @default.
• W4297460343 cites W3135168675 @default.
• W4297460343 cites W3159006653 @default.
• W4297460343 cites W3174088366 @default.
• W4297460343 cites W3182125646 @default.
• W4297460343 cites W3189845806 @default.
• W4297460343 cites W3217020089 @default.
• W4297460343 cites W3127587999 @default.
• W4297460343 doi "https://doi.org/10.1094/pdis-02-22-0386-re" @default.
• W4297460343 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36167511" @default.
• W4297460343 hasPublicationYear "2023" @default.
• W4297460343 type Work @default.
• W4297460343 citedByCount "2" @default.
• W4297460343 countsByYear W42974603432023 @default.
• W4297460343 crossrefType "journal-article" @default.
• W4297460343 hasAuthorship W4297460343A5006760859 @default.
• W4297460343 hasAuthorship W4297460343A5016299379 @default.
• W4297460343 hasAuthorship W4297460343A5038787515 @default.
• W4297460343 hasAuthorship W4297460343A5058287391 @default.
• W4297460343 hasAuthorship W4297460343A5070705794 @default.
• W4297460343 hasAuthorship W4297460343A5072172552 @default.
• W4297460343 hasAuthorship W4297460343A5074342094 @default.
• W4297460343 hasAuthorship W4297460343A5085226903 @default.
• W4297460343 hasAuthorship W4297460343A5086600889 @default.
• W4297460343 hasConcept C104317684 @default.
• W4297460343 hasConcept C139713532 @default.
• W4297460343 hasConcept C153911025 @default.
• W4297460343 hasConcept C159047783 @default.
• W4297460343 hasConcept C18903297 @default.
• W4297460343 hasConcept C2776919830 @default.
• W4297460343 hasConcept C2778830712 @default.
• W4297460343 hasConcept C49105822 @default.
• W4297460343 hasConcept C54355233 @default.
• W4297460343 hasConcept C86803240 @default.
• W4297460343 hasConceptScore W4297460343C104317684 @default.
• W4297460343 hasConceptScore W4297460343C139713532 @default.
• W4297460343 hasConceptScore W4297460343C153911025 @default.
• W4297460343 hasConceptScore W4297460343C159047783 @default.

• next