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• W4297491323 abstract "Human B cells encompass functionally diverse lineages and phenotypic states that contribute to protective as well as pathogenic responses. Epstein-Barr virus (EBV) provides a unique lens for studying heterogeneous B cell responses, given its adaptation to manipulate intrinsic cell programming. EBV promotes the activation, proliferation, and eventual outgrowth of host B cells as immortalized lymphoblastoid cell lines (LCLs) in vitro, which provide a foundational model of viral latency and lymphomagenesis. Although cellular responses and outcomes of infection can vary significantly within populations, investigations that capture genome-wide perspectives of this variation at single-cell resolution are in nascent stages. We have recently used single-cell approaches to identify EBV-mediated B cell heterogeneity in de novo infection and within LCLs, underscoring the dynamic and complex qualities of latent infection rather than a singular, static infection state. Here, we expand upon these findings with functional characterizations of EBV-induced dynamic phenotypes that mimic B cell immune responses. We found that distinct subpopulations isolated from LCLs could completely reconstitute the full phenotypic spectrum of their parental lines. In conjunction with conserved patterns of cell state diversity identified within scRNA-seq data, these data support a model in which EBV continuously drives recurrent B cell entry, progression through, and egress from the Germinal Center (GC) reaction. This perpetual GC also generates tangent cell fate trajectories including terminal plasmablast differentiation, which constitutes a replicative cul-de-sac for EBV from which lytic reactivation provides escape. Furthermore, we found that both established EBV latency and de novo infection support the development of cells with features of atypical memory B cells, which have been broadly associated with autoimmune disorders. Treatment of LCLs with TLR7 agonist or IL-21 was sufficient to generate an increased frequency of IgD-/CD27-/CD23-/CD38+/CD138+ plasmablasts. Separately, de novo EBV infection led to the development of CXCR3+/CD11c+/FCRL4+ B cells within days, providing evidence for possible T cell-independent origins of a recently described EBV-associated neuroinvasive CXCR3+ B cell subset in patients with multiple sclerosis. Collectively, this work reveals unexpected virus-driven complexity across infected cell populations and highlights potential roles of EBV in mediating or priming foundational aspects of virus-associated immune cell dysfunction in disease." @default.
• W4297491323 created "2022-09-29" @default.
• W4297491323 creator A5045212564 @default.
• W4297491323 creator A5066575551 @default.
• W4297491323 creator A5074708582 @default.
• W4297491323 creator A5084068646 @default.
• W4297491323 date "2022-09-28" @default.
• W4297491323 modified "2023-10-01" @default.
• W4297491323 title "Epstein-Barr virus perpetuates B cell germinal center dynamics and generation of autoimmune-associated phenotypes in vitro" @default.
• W4297491323 cites W1488792567 @default.
• W4297491323 cites W1489865648 @default.
• W4297491323 cites W1510154907 @default.
• W4297491323 cites W1544117098 @default.
• W4297491323 cites W1558437093 @default.
• W4297491323 cites W1562827330 @default.
• W4297491323 cites W1578954095 @default.
• W4297491323 cites W1590112381 @default.
• W4297491323 cites W1624278566 @default.
• W4297491323 cites W1724492762 @default.
• W4297491323 cites W1812374189 @default.
• W4297491323 cites W1898667381 @default.
• W4297491323 cites W1912140146 @default.
• W4297491323 cites W1963945655 @default.
• W4297491323 cites W1967278331 @default.
• W4297491323 cites W1967738350 @default.
• W4297491323 cites W1970195713 @default.
• W4297491323 cites W1972167997 @default.
• W4297491323 cites W1979401674 @default.
• W4297491323 cites W1980442677 @default.
• W4297491323 cites W1981006469 @default.
• W4297491323 cites W1981243648 @default.
• W4297491323 cites W1983601844 @default.
• W4297491323 cites W1984883254 @default.
• W4297491323 cites W1987651703 @default.
• W4297491323 cites W1987654323 @default.
• W4297491323 cites W1988622164 @default.
• W4297491323 cites W1997105134 @default.
• W4297491323 cites W2000584697 @default.
• W4297491323 cites W2004165973 @default.
• W4297491323 cites W2008697983 @default.
• W4297491323 cites W2015278968 @default.
• W4297491323 cites W2018012447 @default.
• W4297491323 cites W2019009797 @default.
• W4297491323 cites W2019044913 @default.
• W4297491323 cites W2019979528 @default.
• W4297491323 cites W2023509216 @default.
• W4297491323 cites W2025042617 @default.
• W4297491323 cites W2026639110 @default.
• W4297491323 cites W2026908402 @default.
• W4297491323 cites W2035585065 @default.
• W4297491323 cites W2038832337 @default.
• W4297491323 cites W2040783678 @default.
• W4297491323 cites W2045430347 @default.
• W4297491323 cites W2045537928 @default.
• W4297491323 cites W2047193748 @default.
• W4297491323 cites W2054492080 @default.
• W4297491323 cites W2057749060 @default.
• W4297491323 cites W2069089843 @default.
• W4297491323 cites W2070106785 @default.
• W4297491323 cites W2070254656 @default.
• W4297491323 cites W2070872496 @default.
• W4297491323 cites W2071775939 @default.
• W4297491323 cites W2073989067 @default.
• W4297491323 cites W2074795687 @default.
• W4297491323 cites W2075810563 @default.
• W4297491323 cites W2079053367 @default.
• W4297491323 cites W2082538818 @default.
• W4297491323 cites W2083787944 @default.
• W4297491323 cites W2084669174 @default.
• W4297491323 cites W2088401014 @default.
• W4297491323 cites W2088559118 @default.
• W4297491323 cites W2090919307 @default.
• W4297491323 cites W2095572685 @default.
• W4297491323 cites W2100025622 @default.
• W4297491323 cites W2101092447 @default.
• W4297491323 cites W2106295967 @default.
• W4297491323 cites W2107244203 @default.
• W4297491323 cites W2110840300 @default.
• W4297491323 cites W2110853268 @default.
• W4297491323 cites W2118425309 @default.
• W4297491323 cites W2120679720 @default.
• W4297491323 cites W2123001315 @default.
• W4297491323 cites W2123029438 @default.
• W4297491323 cites W2129003247 @default.
• W4297491323 cites W2139954541 @default.
• W4297491323 cites W2141327215 @default.
• W4297491323 cites W2141616119 @default.
• W4297491323 cites W2147246240 @default.
• W4297491323 cites W2147638701 @default.
• W4297491323 cites W2147641075 @default.
• W4297491323 cites W2148498695 @default.
• W4297491323 cites W2151473316 @default.
• W4297491323 cites W2151633047 @default.
• W4297491323 cites W2155605663 @default.
• W4297491323 cites W2157037109 @default.
• W4297491323 cites W2162538131 @default.
• W4297491323 cites W2164492333 @default.
• W4297491323 cites W2165371767 @default.
• W4297491323 cites W2165696541 @default.
• W4297491323 cites W2168531609 @default.

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