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• W4297497241 endingPage "159096" @default.
• W4297497241 startingPage "159096" @default.
• W4297497241 abstract "Antibiotic resistance genes (ARGs) have been regarded as emerging pollutants due to their potential risk of resistance. Horizontal gene transfer (HGT) is the main pathway for ARGs to lead to environmental threats. Therefore, the inhabitation of ARGs' HGT can effectively inhibit ARGs' potential drug resistance risk within a single strain. In this paper, the characteristics of ARGs' HGT in paddy and dryland soils were identified and regulated by a combination of ARGs' HGT feature identification, transfer mechanism analysis and transfer process regulation. The homology modeling algorithm was used to simulate the construction of the Tn5 plasmid transposase of Escherichia coli (E. coli) for identifying ARGs' HGT characteristics. The GCG (212.617 Å) was thus determined as the target codon. Through integrated computer-based methods, results showed that the most important environmental disturbance factors for the HGT of ARGs in the paddy and dryland soils were rough farmyard manure/sewage irrigation and mining pollution, respectively. Under the disturbance of key environmental factors, the inhibitory effect of HGT of ARGs in paddy and dryland soil was reduced by 35.01 % and 34.74 %, respectively. Results demonstrated that the proposed theoretical mechanism and control strategies could effectively inhibit the HGT of E. coli ARGs in the soil environment." @default.
• W4297497241 created "2022-09-29" @default.
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• W4297497241 date "2023-01-01" @default.
• W4297497241 modified "2023-10-10" @default.
• W4297497241 title "Regulatory strategies for inhibiting horizontal gene transfer of ARGs in paddy and dryland soil through computer-based methods" @default.
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• W4297497241 doi "https://doi.org/10.1016/j.scitotenv.2022.159096" @default.
• W4297497241 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36181826" @default.
• W4297497241 hasPublicationYear "2023" @default.
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