Matches in SemOpenAlex for { <https://semopenalex.org/work/W4297876517> ?p ?o ?g. }
- W4297876517 endingPage "197" @default.
- W4297876517 startingPage "197" @default.
- W4297876517 abstract "<ns3:p><ns3:bold>Background</ns3:bold>: Progression through mammalian embryogenesis involves many interacting cell types and multiple differentiating cell lineages. Quantitative polymerase chain reaction (qPCR) analysis of gene expression in the developing embryo is a valuable tool for deciphering these processes, but normalisation to stably-expressed reference genes is essential for such analyses. Gene expression patterns change globally and dramatically as embryonic development proceeds, rendering identification of consistently appropriate reference genes challenging.</ns3:p><ns3:p> <ns3:bold>Methods</ns3:bold>: We have investigated expression stability in mouse embryos from mid to late gestation (E11.5–E18.5), both at the whole-embryo level, and within the head and forelimb specifically, using 15 candidate reference genes ( <ns3:italic>ACTB, 18S, SDHA, GAPDH, HTATSF1, CDC40, RPL13A, CSNK2A2, AP3D1, HPRT1, CYC1, EIF4A, UBC, B2M </ns3:italic>and <ns3:italic>PAK1IP1</ns3:italic>), and four complementary algorithms (geNorm, Normfinder, Bestkeeper and deltaCt).</ns3:p><ns3:p> <ns3:bold>Results</ns3:bold>: Unexpectedly, all methods suggest that many genes within our candidate panel are acceptable references, though <ns3:italic>AP3D1</ns3:italic>, <ns3:italic>RPL13A</ns3:italic> and <ns3:italic>PAK1IP1</ns3:italic> are the strongest performing genes overall (scoring highly in whole embryos, heads or forelimbs alone, and in all samples collectively). <ns3:italic>HPRT1</ns3:italic> and <ns3:italic>B2M</ns3:italic> are conversely poor choices, and show strong developmental regulation. We further show that normalisation using our three highest-scoring references can reveal subtle patterns of developmental expression even in genes ostensibly ranked as acceptably stable ( <ns3:italic>CDC40</ns3:italic>, <ns3:italic>HTATSF1</ns3:italic>).</ns3:p><ns3:p> <ns3:bold>Conclusion</ns3:bold>: <ns3:italic>AP3D1</ns3:italic>, <ns3:italic>RPL13A</ns3:italic> and <ns3:italic>PAK1IP1</ns3:italic> represent universally suitable reference genes for expression studies in the E11.5-E18.5 mouse embryo.</ns3:p>" @default.
- W4297876517 created "2022-10-01" @default.
- W4297876517 creator A5021347280 @default.
- W4297876517 creator A5042740304 @default.
- W4297876517 creator A5089252543 @default.
- W4297876517 date "2022-09-21" @default.
- W4297876517 modified "2023-09-26" @default.
- W4297876517 title "Identification of qPCR reference genes suitable for normalising gene expression in the developing mouse embryo" @default.
- W4297876517 cites W1577577364 @default.
- W4297876517 cites W1763659965 @default.
- W4297876517 cites W1973556963 @default.
- W4297876517 cites W1984306234 @default.
- W4297876517 cites W1986080495 @default.
- W4297876517 cites W1987517014 @default.
- W4297876517 cites W1988397986 @default.
- W4297876517 cites W1990786422 @default.
- W4297876517 cites W1995131166 @default.
- W4297876517 cites W2007760130 @default.
- W4297876517 cites W2009957456 @default.
- W4297876517 cites W2013581570 @default.
- W4297876517 cites W2013990566 @default.
- W4297876517 cites W2017932771 @default.
- W4297876517 cites W2031766506 @default.
- W4297876517 cites W2035963479 @default.
- W4297876517 cites W2040785797 @default.
- W4297876517 cites W2042222943 @default.
- W4297876517 cites W2044509030 @default.
- W4297876517 cites W2053551121 @default.
- W4297876517 cites W2054539933 @default.
- W4297876517 cites W2064290205 @default.
- W4297876517 cites W2073930681 @default.
- W4297876517 cites W2077765163 @default.
- W4297876517 cites W2085775253 @default.
- W4297876517 cites W2091955737 @default.
- W4297876517 cites W2099824652 @default.
- W4297876517 cites W2105423509 @default.
- W4297876517 cites W2106257400 @default.
- W4297876517 cites W2111427334 @default.
- W4297876517 cites W2120876378 @default.
- W4297876517 cites W2120920194 @default.
- W4297876517 cites W2127417296 @default.
- W4297876517 cites W2133481779 @default.
- W4297876517 cites W2146864828 @default.
- W4297876517 cites W2150980806 @default.
- W4297876517 cites W2158003869 @default.
- W4297876517 cites W2163477673 @default.
- W4297876517 cites W2168420558 @default.
- W4297876517 cites W2271925893 @default.
- W4297876517 cites W2425836780 @default.
- W4297876517 cites W2564167865 @default.
- W4297876517 cites W2583128767 @default.
- W4297876517 cites W2719903628 @default.
- W4297876517 cites W2754348497 @default.
- W4297876517 cites W2788713419 @default.
- W4297876517 cites W2795274478 @default.
- W4297876517 cites W2795471900 @default.
- W4297876517 cites W2807176168 @default.
- W4297876517 cites W2886169797 @default.
- W4297876517 cites W2901757853 @default.
- W4297876517 cites W2913191529 @default.
- W4297876517 cites W2916020270 @default.
- W4297876517 cites W2954043568 @default.
- W4297876517 cites W3004833389 @default.
- W4297876517 cites W3010552888 @default.
- W4297876517 cites W3045864178 @default.
- W4297876517 cites W4210268651 @default.
- W4297876517 cites W4240939572 @default.
- W4297876517 cites W982821278 @default.
- W4297876517 doi "https://doi.org/10.12688/wellcomeopenres.16972.2" @default.
- W4297876517 hasPublicationYear "2022" @default.
- W4297876517 type Work @default.
- W4297876517 citedByCount "0" @default.
- W4297876517 crossrefType "journal-article" @default.
- W4297876517 hasAuthorship W4297876517A5021347280 @default.
- W4297876517 hasAuthorship W4297876517A5042740304 @default.
- W4297876517 hasAuthorship W4297876517A5089252543 @default.
- W4297876517 hasBestOaLocation W42978765171 @default.
- W4297876517 hasConcept C104317684 @default.
- W4297876517 hasConcept C132379496 @default.
- W4297876517 hasConcept C150194340 @default.
- W4297876517 hasConcept C2522874641 @default.
- W4297876517 hasConcept C2776408679 @default.
- W4297876517 hasConcept C2779148672 @default.
- W4297876517 hasConcept C54355233 @default.
- W4297876517 hasConcept C70721500 @default.
- W4297876517 hasConcept C86803240 @default.
- W4297876517 hasConceptScore W4297876517C104317684 @default.
- W4297876517 hasConceptScore W4297876517C132379496 @default.
- W4297876517 hasConceptScore W4297876517C150194340 @default.
- W4297876517 hasConceptScore W4297876517C2522874641 @default.
- W4297876517 hasConceptScore W4297876517C2776408679 @default.
- W4297876517 hasConceptScore W4297876517C2779148672 @default.
- W4297876517 hasConceptScore W4297876517C54355233 @default.
- W4297876517 hasConceptScore W4297876517C70721500 @default.
- W4297876517 hasConceptScore W4297876517C86803240 @default.
- W4297876517 hasFunder F4320311904 @default.
- W4297876517 hasLocation W42978765171 @default.
- W4297876517 hasLocation W42978765172 @default.