Matches in SemOpenAlex for { <https://semopenalex.org/work/W4297884015> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W4297884015 abstract "Background: Metastatic breast cancer (mBC) is a heterogeneous disease with varying responses to treatments and clinical outcomes, still requiring the identification of reliable predictive biomarkers. In this context, liquid biopsy has emerged as a powerful tool to assess in real-time the evolving landscape of cancer, which is both orchestrated by the metastatic process and immune-surveillance mechanisms. Thus, we investigated circulating tumor cells (CTCs) coupled with peripheral T-cell immunity to uncover their potential clinical relevance in mBC.Methods: A cohort of 20 mBC patients was evaluated, before and one month after starting therapy, through the following liquid biopsy approaches: CTCs enumerated by a metabolism-based assay, T-cell responses against tumor-associated antigens (TAA) characterized by interferon-γ enzyme-linked immunosorbent spot (ELISpot), and the T-cell receptor (TCR) repertoire investigated by a targeted next-generation sequencing technique. TCR repertoire features were characterized by the Morisita’s overlap and the Productive Simpson Clonality indexes, and the TCR richness. Differences between groups were calculated by Fisher’s, Mann-Whitney or Kruskal-Wallis test, as appropriate. Prognostic data analysis was estimated by Kaplan-Meier method.Results: Stratifying patients for their prognostic level of 6 CTCs before therapy, TAA specific T-cell responses were detected only in patients with a low CTC level. By analyzing the TCR repertoire, the highest TCR clonality was observed in the case of CTCs under the cut-off and a positive ELISpot response (p=0.03). Whereas, at follow-up, patients showing a good clinical response coupled with a low number of CTCs were characterized by the most elevated TCR clonality (pConclusion: These data, whether validated in a larger cohort of patients, suggest that the combined analysis of CTCs and circulating anti-tumor T-cell immunity could represent a valuable immune-oncological biomarker for the liquid biopsy field. The clinical application of this promising tool could improve the management of mBC patients, especially in the setting of immunotherapy, a rising approach for BC treatment requiring reliable predictive biomarkers." @default.
- W4297884015 created "2022-10-01" @default.
- W4297884015 creator A5001656227 @default.
- W4297884015 creator A5010211368 @default.
- W4297884015 creator A5029394520 @default.
- W4297884015 creator A5034715606 @default.
- W4297884015 creator A5044117806 @default.
- W4297884015 creator A5050082318 @default.
- W4297884015 creator A5059663758 @default.
- W4297884015 creator A5072157941 @default.
- W4297884015 creator A5072639560 @default.
- W4297884015 creator A5075915013 @default.
- W4297884015 creator A5090069241 @default.
- W4297884015 date "2022-09-12" @default.
- W4297884015 modified "2023-09-25" @default.
- W4297884015 title "Clinical relevance of the combined analysis of circulating tumor cells and anti-tumor T-cell immunity in metastatic breast cancer patients" @default.
- W4297884015 doi "https://doi.org/10.21417/em2022fo" @default.
- W4297884015 hasPublicationYear "2022" @default.
- W4297884015 type Work @default.
- W4297884015 citedByCount "0" @default.
- W4297884015 crossrefType "dataset" @default.
- W4297884015 hasAuthorship W4297884015A5001656227 @default.
- W4297884015 hasAuthorship W4297884015A5010211368 @default.
- W4297884015 hasAuthorship W4297884015A5029394520 @default.
- W4297884015 hasAuthorship W4297884015A5034715606 @default.
- W4297884015 hasAuthorship W4297884015A5044117806 @default.
- W4297884015 hasAuthorship W4297884015A5050082318 @default.
- W4297884015 hasAuthorship W4297884015A5059663758 @default.
- W4297884015 hasAuthorship W4297884015A5072157941 @default.
- W4297884015 hasAuthorship W4297884015A5072639560 @default.
- W4297884015 hasAuthorship W4297884015A5075915013 @default.
- W4297884015 hasAuthorship W4297884015A5090069241 @default.
- W4297884015 hasConcept C121608353 @default.
- W4297884015 hasConcept C126322002 @default.
- W4297884015 hasConcept C143998085 @default.
- W4297884015 hasConcept C203014093 @default.
- W4297884015 hasConcept C2775930923 @default.
- W4297884015 hasConcept C2779013556 @default.
- W4297884015 hasConcept C2779341262 @default.
- W4297884015 hasConcept C2986637895 @default.
- W4297884015 hasConcept C3020616263 @default.
- W4297884015 hasConcept C3020653616 @default.
- W4297884015 hasConcept C502942594 @default.
- W4297884015 hasConcept C530470458 @default.
- W4297884015 hasConcept C61238886 @default.
- W4297884015 hasConcept C63363279 @default.
- W4297884015 hasConcept C71924100 @default.
- W4297884015 hasConcept C8891405 @default.
- W4297884015 hasConceptScore W4297884015C121608353 @default.
- W4297884015 hasConceptScore W4297884015C126322002 @default.
- W4297884015 hasConceptScore W4297884015C143998085 @default.
- W4297884015 hasConceptScore W4297884015C203014093 @default.
- W4297884015 hasConceptScore W4297884015C2775930923 @default.
- W4297884015 hasConceptScore W4297884015C2779013556 @default.
- W4297884015 hasConceptScore W4297884015C2779341262 @default.
- W4297884015 hasConceptScore W4297884015C2986637895 @default.
- W4297884015 hasConceptScore W4297884015C3020616263 @default.
- W4297884015 hasConceptScore W4297884015C3020653616 @default.
- W4297884015 hasConceptScore W4297884015C502942594 @default.
- W4297884015 hasConceptScore W4297884015C530470458 @default.
- W4297884015 hasConceptScore W4297884015C61238886 @default.
- W4297884015 hasConceptScore W4297884015C63363279 @default.
- W4297884015 hasConceptScore W4297884015C71924100 @default.
- W4297884015 hasConceptScore W4297884015C8891405 @default.
- W4297884015 hasLocation W42978840151 @default.
- W4297884015 hasOpenAccess W4297884015 @default.
- W4297884015 hasPrimaryLocation W42978840151 @default.
- W4297884015 hasRelatedWork W2006435270 @default.
- W4297884015 hasRelatedWork W2071252006 @default.
- W4297884015 hasRelatedWork W2377793687 @default.
- W4297884015 hasRelatedWork W2395906155 @default.
- W4297884015 hasRelatedWork W2407256926 @default.
- W4297884015 hasRelatedWork W2979892935 @default.
- W4297884015 hasRelatedWork W3008690400 @default.
- W4297884015 hasRelatedWork W3143003092 @default.
- W4297884015 hasRelatedWork W4251597418 @default.
- W4297884015 hasRelatedWork W73860091 @default.
- W4297884015 isParatext "false" @default.
- W4297884015 isRetracted "false" @default.
- W4297884015 workType "dataset" @default.