FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4297884550> ?p ?o ?g. }

• W4297884550 abstract "Abstract Backgrounds Rapid separation methods with fusion of the affinity tags have been developed. However, the affinity tag for simply and cheaply separating the fused target protein is still lacking. Results Separation conditions for the human annexin A1 (hanA1) tagged emerald green fluorescent protein (EmGFP) in Escherichia coli cytoplasm were optimized via precipitation with CaCl2 and re-solubilization with EDTA-Na2. Combination of the affinity precipitation with each of three affinity purification approaches increased the hanA1-EmGFP purity, and on-resin cleavage facilitated release of tag-free EmGFP. With addition of Triton X-100 to the culture, the fused EmGFP, red fluorescent protein mCherry, or the FMN-dependent fluorescent protein LOV, was also prepared with increasing CaCl2concentrations after it was secreted to the culture from E. coli , whereas the hanA1-EmGFP fused to the amyQ signal sequence was insolubly produced in Bacillus subtilis cells, and the hanA1-EmGFP fused to the α signal peptide produced in Pichia pastoris was unable to be secreted to the culture. The affinity separation was visualized by use of three fluorescent proteins including the EmGFP, mCherry and LOV, and two colored proteins including a bacterial hemoglobin, and maize sirohydrochlorin ferrochelatase (mSF) showing brown containing the [2Fe–2S] cluster. The added EDTA-Na2disrupted the mSF structure, and inhibited activities of the selected four metal-dependent enzymes, but showed little impact on two specific proteases for cleaving the fusion proteins. After affinity precipitation, the tagged lysine decarboxylase was prepared as cross-linked enzyme aggregates. Conclusion The hanA1 tag is ideal for simple, rapid and cost-effective separation of the target proteins via intracellular and extracellular production in E. coli . This tag is also used for further affinity purification of the selected proteins and enzymes potentially applied in industry and diagnosis." @default.
• W4297884550 created "2022-10-01" @default.
• W4297884550 creator A5020993466 @default.
• W4297884550 creator A5021725931 @default.
• W4297884550 creator A5028364893 @default.
• W4297884550 creator A5038222109 @default.
• W4297884550 creator A5066136832 @default.
• W4297884550 creator A5068354146 @default.
• W4297884550 creator A5070564080 @default.
• W4297884550 date "2022-09-20" @default.
• W4297884550 modified "2023-10-07" @default.
• W4297884550 title "Investigating the applicability of human annexin A1 as an affinity tag for separation and purification of the target proteins" @default.
• W4297884550 cites W1494791252 @default.
• W4297884550 cites W1932669647 @default.
• W4297884550 cites W1966451156 @default.
• W4297884550 cites W1970265878 @default.
• W4297884550 cites W1970609835 @default.
• W4297884550 cites W1973199370 @default.
• W4297884550 cites W1976697284 @default.
• W4297884550 cites W1985991048 @default.
• W4297884550 cites W1993199745 @default.
• W4297884550 cites W1995087209 @default.
• W4297884550 cites W1998834713 @default.
• W4297884550 cites W2004144252 @default.
• W4297884550 cites W2008737872 @default.
• W4297884550 cites W2015166281 @default.
• W4297884550 cites W2018713010 @default.
• W4297884550 cites W2026068832 @default.
• W4297884550 cites W2026523419 @default.
• W4297884550 cites W2027625719 @default.
• W4297884550 cites W2027908623 @default.
• W4297884550 cites W2030506720 @default.
• W4297884550 cites W2031364768 @default.
• W4297884550 cites W2036184150 @default.
• W4297884550 cites W2037955590 @default.
• W4297884550 cites W2043843363 @default.
• W4297884550 cites W2044571381 @default.
• W4297884550 cites W2057192639 @default.
• W4297884550 cites W2061961731 @default.
• W4297884550 cites W2065575488 @default.
• W4297884550 cites W2070762752 @default.
• W4297884550 cites W2072198224 @default.
• W4297884550 cites W2091844380 @default.
• W4297884550 cites W2092796622 @default.
• W4297884550 cites W2095373397 @default.
• W4297884550 cites W2120292671 @default.
• W4297884550 cites W2120340514 @default.
• W4297884550 cites W2130583289 @default.
• W4297884550 cites W2160713787 @default.
• W4297884550 cites W2165832887 @default.
• W4297884550 cites W2167783675 @default.
• W4297884550 cites W2171475828 @default.
• W4297884550 cites W2277535723 @default.
• W4297884550 cites W2358813103 @default.
• W4297884550 cites W2534380384 @default.
• W4297884550 cites W2586068119 @default.
• W4297884550 cites W2587418219 @default.
• W4297884550 cites W2595826119 @default.
• W4297884550 cites W2607581629 @default.
• W4297884550 cites W2607883363 @default.
• W4297884550 cites W2608449006 @default.
• W4297884550 cites W2793497013 @default.
• W4297884550 cites W2802962010 @default.
• W4297884550 cites W2821898572 @default.
• W4297884550 cites W2885499617 @default.
• W4297884550 cites W3009570736 @default.
• W4297884550 cites W3097149349 @default.
• W4297884550 cites W4224047228 @default.
• W4297884550 doi "https://doi.org/10.21203/rs.3.rs-2070567/v1" @default.
• W4297884550 hasPublicationYear "2022" @default.
• W4297884550 type Work @default.
• W4297884550 citedByCount "0" @default.
• W4297884550 crossrefType "posted-content" @default.
• W4297884550 hasAuthorship W4297884550A5020993466 @default.
• W4297884550 hasAuthorship W4297884550A5021725931 @default.
• W4297884550 hasAuthorship W4297884550A5028364893 @default.
• W4297884550 hasAuthorship W4297884550A5038222109 @default.
• W4297884550 hasAuthorship W4297884550A5066136832 @default.
• W4297884550 hasAuthorship W4297884550A5068354146 @default.
• W4297884550 hasAuthorship W4297884550A5070564080 @default.
• W4297884550 hasBestOaLocation W42978845501 @default.
• W4297884550 hasConcept C104317684 @default.
• W4297884550 hasConcept C10858879 @default.
• W4297884550 hasConcept C123894998 @default.
• W4297884550 hasConcept C142613039 @default.
• W4297884550 hasConcept C153911025 @default.
• W4297884550 hasConcept C156904656 @default.
• W4297884550 hasConcept C179247698 @default.
• W4297884550 hasConcept C181199279 @default.
• W4297884550 hasConcept C185592680 @default.
• W4297884550 hasConcept C2777272437 @default.
• W4297884550 hasConcept C2779260011 @default.
• W4297884550 hasConcept C2780430158 @default.
• W4297884550 hasConcept C2781372317 @default.
• W4297884550 hasConcept C40767141 @default.
• W4297884550 hasConcept C43617362 @default.
• W4297884550 hasConcept C523546767 @default.
• W4297884550 hasConcept C54355233 @default.
• W4297884550 hasConcept C547475151 @default.
• W4297884550 hasConcept C55493867 @default.

• next