Matches in SemOpenAlex for { <https://semopenalex.org/work/W4298088361> ?p ?o ?g. }
- W4298088361 endingPage "18396" @default.
- W4298088361 startingPage "18387" @default.
- W4298088361 abstract "Bioconjugation is a powerful protein modification strategy to improve protein properties. Herein, we report mechano-bioconjugation as a novel approach to empower fusion protein therapeutics and demonstrate its utility by a protein heterocatenane (cat-IFN-ABD) containing interferon-α2b (IFN) mechanically interlocked with a consensus albumin-binding domain (ABD). The conjugate was selectively synthesized in cellulo following a cascade of post-translational events using a pair of heterodimerizing p53dim variants and two orthogonal split-intein reactions. The catenane topology was proven by combined techniques of LC-MS, SDS-PAGE, SEC, and controlled proteolytic digestion. Not only did cat-IFN-ABD retain activities comparable to those of the wild-type IFN and ABD, the conjugate also exhibited enhanced aggregation resistance and prolonged circulation time over the simple linear and cyclic fusions. Consequently, cat-IFN-ABD potently inhibited tumor growth in the mouse xenograft model. Therefore, mechano-bioconjugation by catenation accomplishes function integration with additional benefits, providing an alternative pathway for developing advanced protein therapeutics." @default.
- W4298088361 created "2022-10-01" @default.
- W4298088361 creator A5015317478 @default.
- W4298088361 creator A5024819274 @default.
- W4298088361 creator A5041977378 @default.
- W4298088361 creator A5052453719 @default.
- W4298088361 creator A5052777307 @default.
- W4298088361 creator A5053447705 @default.
- W4298088361 creator A5053961855 @default.
- W4298088361 creator A5054506269 @default.
- W4298088361 date "2022-09-30" @default.
- W4298088361 modified "2023-10-15" @default.
- W4298088361 title "Mechano-bioconjugation Strategy Empowering Fusion Protein Therapeutics with Aggregation Resistance, Prolonged Circulation, and Enhanced Antitumor Efficacy" @default.
- W4298088361 cites W1458390031 @default.
- W4298088361 cites W1611944043 @default.
- W4298088361 cites W1839516892 @default.
- W4298088361 cites W1873045526 @default.
- W4298088361 cites W1975686864 @default.
- W4298088361 cites W1977337377 @default.
- W4298088361 cites W1978329469 @default.
- W4298088361 cites W1980402791 @default.
- W4298088361 cites W1984844116 @default.
- W4298088361 cites W1987259801 @default.
- W4298088361 cites W2027268124 @default.
- W4298088361 cites W2035776098 @default.
- W4298088361 cites W2047188856 @default.
- W4298088361 cites W2047329659 @default.
- W4298088361 cites W2051998923 @default.
- W4298088361 cites W2077816930 @default.
- W4298088361 cites W2081306620 @default.
- W4298088361 cites W2086040562 @default.
- W4298088361 cites W2094058129 @default.
- W4298088361 cites W2094654556 @default.
- W4298088361 cites W2131209038 @default.
- W4298088361 cites W2142337801 @default.
- W4298088361 cites W2145720841 @default.
- W4298088361 cites W2152001313 @default.
- W4298088361 cites W2153258430 @default.
- W4298088361 cites W2156842818 @default.
- W4298088361 cites W2159330187 @default.
- W4298088361 cites W2286914510 @default.
- W4298088361 cites W2300352749 @default.
- W4298088361 cites W2300928680 @default.
- W4298088361 cites W2302120143 @default.
- W4298088361 cites W2315302880 @default.
- W4298088361 cites W2395806397 @default.
- W4298088361 cites W2470831393 @default.
- W4298088361 cites W2472635460 @default.
- W4298088361 cites W2497556825 @default.
- W4298088361 cites W2534830410 @default.
- W4298088361 cites W2536988219 @default.
- W4298088361 cites W2552023306 @default.
- W4298088361 cites W2594458869 @default.
- W4298088361 cites W2612426099 @default.
- W4298088361 cites W2752929898 @default.
- W4298088361 cites W2775819204 @default.
- W4298088361 cites W2777336123 @default.
- W4298088361 cites W2779719913 @default.
- W4298088361 cites W2794080334 @default.
- W4298088361 cites W2800128426 @default.
- W4298088361 cites W2887418029 @default.
- W4298088361 cites W2904421759 @default.
- W4298088361 cites W2909762186 @default.
- W4298088361 cites W2917928050 @default.
- W4298088361 cites W2920526722 @default.
- W4298088361 cites W2943121271 @default.
- W4298088361 cites W2947244729 @default.
- W4298088361 cites W2959145534 @default.
- W4298088361 cites W3020696431 @default.
- W4298088361 cites W3027069596 @default.
- W4298088361 cites W3033301893 @default.
- W4298088361 cites W3034500342 @default.
- W4298088361 cites W3038160028 @default.
- W4298088361 cites W3067029739 @default.
- W4298088361 cites W3088402839 @default.
- W4298088361 cites W3093297066 @default.
- W4298088361 cites W3109975779 @default.
- W4298088361 cites W3111668408 @default.
- W4298088361 cites W3193427447 @default.
- W4298088361 cites W3204247169 @default.
- W4298088361 cites W3205136458 @default.
- W4298088361 cites W4200463757 @default.
- W4298088361 cites W4213162043 @default.
- W4298088361 cites W4225102336 @default.
- W4298088361 cites W4234457620 @default.
- W4298088361 cites W4241290724 @default.
- W4298088361 cites W986179422 @default.
- W4298088361 doi "https://doi.org/10.1021/jacs.2c06532" @default.
- W4298088361 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36178288" @default.
- W4298088361 hasPublicationYear "2022" @default.
- W4298088361 type Work @default.
- W4298088361 citedByCount "2" @default.
- W4298088361 countsByYear W42980883612022 @default.
- W4298088361 countsByYear W42980883612023 @default.
- W4298088361 crossrefType "journal-article" @default.
- W4298088361 hasAuthorship W4298088361A5015317478 @default.
- W4298088361 hasAuthorship W4298088361A5024819274 @default.
- W4298088361 hasAuthorship W4298088361A5041977378 @default.