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- W4298175786 abstract "Abstract We used single-strand conformational polymorphism (SSCP) to determine apolipoprotein E (Apo E) genotypes in 47 individuals. A 295-base-pair (bp) DNA fragment coding for amino acid residues 80-178 of the Apo E protein gave distinct patterns for the three alleles. When we used SSCP to determine the Apo E polymorphism of five individuals whose phenotyping results differed from those of genotyping, the SSCP results agreed with the genotyping results obtained by the PCR-based amplification refractory mutation system (ARMS). Because most of the reported rare alleles of the Apo E gene involve mutations of amino acid residues in positions 120-160, our SSCP method is useful for determining rare as well as common alleles." @default.
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- W4298175786 date "1993-10-01" @default.
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- W4298175786 title "Determination of apolipoprotein E genotypes by single-strand conformational polymorphism" @default.
- W4298175786 doi "https://doi.org/10.1093/clinchem/39.10.2121" @default.
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