Matches in SemOpenAlex for { <https://semopenalex.org/work/W4298577950> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W4298577950 abstract "Label-free impedance-based assays are increasingly used to non-invasively study ligand-induced GPCR activation in cell culture experiments. The approach provides real-time cell monitoring with a device-dependent time resolution down to several tens of milliseconds and it is highly automated. However, when sample numbers get high (e.g., dose-response studies for various different ligands), the cost for the disposable electrode arrays as well as the available time resolution for sequential well-by-well recordings may become limiting. Therefore, we here present a serial agonist addition protocol which has the potential to significantly increase the output of label-free GPCR assays. Using the serial agonist addition protocol, a GPCR agonist is added sequentially in increasing concentrations to a single cell layer while continuously monitoring the sample's impedance (agonist mode). With this serial approach, it is now possible to establish a full dose-response curve for a GPCR agonist from just one single cell layer. The serial agonist addition protocol is applicable to different GPCR coupling types, Gq Gi/0 or Gs and it is compatible with recombinant and endogenous expression levels of the receptor under study. Receptor blocking by GPCR antagonists is assessable as well (antagonist mode)." @default.
- W4298577950 created "2022-10-02" @default.
- W4298577950 creator A5005226020 @default.
- W4298577950 creator A5005569192 @default.
- W4298577950 creator A5007797524 @default.
- W4298577950 creator A5016120063 @default.
- W4298577950 creator A5028636451 @default.
- W4298577950 creator A5051453276 @default.
- W4298577950 creator A5073055805 @default.
- W4298577950 creator A5081248127 @default.
- W4298577950 creator A5088053624 @default.
- W4298577950 date "2020-02-21" @default.
- W4298577950 modified "2023-10-17" @default.
- W4298577950 title "Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays" @default.
- W4298577950 doi "https://doi.org/10.3791/60686-v" @default.
- W4298577950 hasPublicationYear "2020" @default.
- W4298577950 type Work @default.
- W4298577950 citedByCount "0" @default.
- W4298577950 crossrefType "journal-article" @default.
- W4298577950 hasAuthorship W4298577950A5005226020 @default.
- W4298577950 hasAuthorship W4298577950A5005569192 @default.
- W4298577950 hasAuthorship W4298577950A5007797524 @default.
- W4298577950 hasAuthorship W4298577950A5016120063 @default.
- W4298577950 hasAuthorship W4298577950A5028636451 @default.
- W4298577950 hasAuthorship W4298577950A5051453276 @default.
- W4298577950 hasAuthorship W4298577950A5073055805 @default.
- W4298577950 hasAuthorship W4298577950A5081248127 @default.
- W4298577950 hasAuthorship W4298577950A5088053624 @default.
- W4298577950 hasConcept C120750228 @default.
- W4298577950 hasConcept C126322002 @default.
- W4298577950 hasConcept C135285700 @default.
- W4298577950 hasConcept C160262196 @default.
- W4298577950 hasConcept C170493617 @default.
- W4298577950 hasConcept C185592680 @default.
- W4298577950 hasConcept C2778938600 @default.
- W4298577950 hasConcept C58732023 @default.
- W4298577950 hasConcept C71924100 @default.
- W4298577950 hasConcept C98274493 @default.
- W4298577950 hasConceptScore W4298577950C120750228 @default.
- W4298577950 hasConceptScore W4298577950C126322002 @default.
- W4298577950 hasConceptScore W4298577950C135285700 @default.
- W4298577950 hasConceptScore W4298577950C160262196 @default.
- W4298577950 hasConceptScore W4298577950C170493617 @default.
- W4298577950 hasConceptScore W4298577950C185592680 @default.
- W4298577950 hasConceptScore W4298577950C2778938600 @default.
- W4298577950 hasConceptScore W4298577950C58732023 @default.
- W4298577950 hasConceptScore W4298577950C71924100 @default.
- W4298577950 hasConceptScore W4298577950C98274493 @default.
- W4298577950 hasIssue "156" @default.
- W4298577950 hasLocation W42985779501 @default.
- W4298577950 hasOpenAccess W4298577950 @default.
- W4298577950 hasPrimaryLocation W42985779501 @default.
- W4298577950 hasRelatedWork W1976548894 @default.
- W4298577950 hasRelatedWork W1979013132 @default.
- W4298577950 hasRelatedWork W2046945670 @default.
- W4298577950 hasRelatedWork W2068497320 @default.
- W4298577950 hasRelatedWork W2071331143 @default.
- W4298577950 hasRelatedWork W2291710622 @default.
- W4298577950 hasRelatedWork W2766465375 @default.
- W4298577950 hasRelatedWork W4252729716 @default.
- W4298577950 hasRelatedWork W4281739791 @default.
- W4298577950 hasRelatedWork W4286856416 @default.
- W4298577950 isParatext "false" @default.
- W4298577950 isRetracted "false" @default.
- W4298577950 workType "article" @default.