Matches in SemOpenAlex for { <https://semopenalex.org/work/W4299109619> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W4299109619 abstract "The limitations of cell-based synthetic biology are becoming increasingly apparent as researchers aim to develop larger and more complex synthetic genetic regulatory circuits. The analysis of synthetic genetic regulatory networks in vivo is time consuming and suffers from a lack of environmental control, with exogenous synthetic components interacting with host processes resulting in undesired behavior. To overcome these issues, cell-free characterization of novel circuitry is becoming more prevalent. In vitro transcription and translation (IVTT) mixtures allow the regulation of the experimental environment and can be optimized for each unique system. The protocols presented here detail the fabrication of a multilayer microfluidic device that can be utilized to sustain IVTT reactions for prolonged durations. In contrast to batch reactions, where resources are depleted over time and (by-) products accumulate, the use of microfluidic devices allows the replenishment of resources as well as the removal of reaction products. In this manner, the cellular environment is emulated by maintaining an out-of-equilibrium environment in which the dynamic behavior of gene circuits can be investigated over extended periods of time. To fully exploit the multilayer microfluidic device, hardware and software have been integrated to automate the IVTT reactions. By combining IVTT reactions with the microfluidic platform presented here, it becomes possible to comprehensively analyze complex network behaviors, furthering our understanding of the mechanisms that regulate cellular processes." @default.
- W4299109619 created "2022-10-02" @default.
- W4299109619 creator A5026856647 @default.
- W4299109619 creator A5032301188 @default.
- W4299109619 creator A5032517726 @default.
- W4299109619 creator A5057058267 @default.
- W4299109619 creator A5080341371 @default.
- W4299109619 creator A5087352392 @default.
- W4299109619 creator A5089850484 @default.
- W4299109619 date "2019-10-06" @default.
- W4299109619 modified "2023-10-16" @default.
- W4299109619 title "A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression" @default.
- W4299109619 doi "https://doi.org/10.3791/59655-v" @default.
- W4299109619 hasPublicationYear "2019" @default.
- W4299109619 type Work @default.
- W4299109619 citedByCount "0" @default.
- W4299109619 crossrefType "journal-article" @default.
- W4299109619 hasAuthorship W4299109619A5026856647 @default.
- W4299109619 hasAuthorship W4299109619A5032301188 @default.
- W4299109619 hasAuthorship W4299109619A5032517726 @default.
- W4299109619 hasAuthorship W4299109619A5057058267 @default.
- W4299109619 hasAuthorship W4299109619A5080341371 @default.
- W4299109619 hasAuthorship W4299109619A5087352392 @default.
- W4299109619 hasAuthorship W4299109619A5089850484 @default.
- W4299109619 hasBestOaLocation W42991096191 @default.
- W4299109619 hasConcept C104317684 @default.
- W4299109619 hasConcept C127413603 @default.
- W4299109619 hasConcept C150194340 @default.
- W4299109619 hasConcept C165696696 @default.
- W4299109619 hasConcept C171250308 @default.
- W4299109619 hasConcept C183696295 @default.
- W4299109619 hasConcept C186060115 @default.
- W4299109619 hasConcept C191908910 @default.
- W4299109619 hasConcept C192562407 @default.
- W4299109619 hasConcept C38652104 @default.
- W4299109619 hasConcept C41008148 @default.
- W4299109619 hasConcept C54355233 @default.
- W4299109619 hasConcept C67339327 @default.
- W4299109619 hasConcept C70721500 @default.
- W4299109619 hasConcept C8673954 @default.
- W4299109619 hasConcept C86803240 @default.
- W4299109619 hasConceptScore W4299109619C104317684 @default.
- W4299109619 hasConceptScore W4299109619C127413603 @default.
- W4299109619 hasConceptScore W4299109619C150194340 @default.
- W4299109619 hasConceptScore W4299109619C165696696 @default.
- W4299109619 hasConceptScore W4299109619C171250308 @default.
- W4299109619 hasConceptScore W4299109619C183696295 @default.
- W4299109619 hasConceptScore W4299109619C186060115 @default.
- W4299109619 hasConceptScore W4299109619C191908910 @default.
- W4299109619 hasConceptScore W4299109619C192562407 @default.
- W4299109619 hasConceptScore W4299109619C38652104 @default.
- W4299109619 hasConceptScore W4299109619C41008148 @default.
- W4299109619 hasConceptScore W4299109619C54355233 @default.
- W4299109619 hasConceptScore W4299109619C67339327 @default.
- W4299109619 hasConceptScore W4299109619C70721500 @default.
- W4299109619 hasConceptScore W4299109619C8673954 @default.
- W4299109619 hasConceptScore W4299109619C86803240 @default.
- W4299109619 hasIssue "152" @default.
- W4299109619 hasLocation W42991096191 @default.
- W4299109619 hasLocation W42991096192 @default.
- W4299109619 hasLocation W42991096193 @default.
- W4299109619 hasLocation W42991096194 @default.
- W4299109619 hasOpenAccess W4299109619 @default.
- W4299109619 hasPrimaryLocation W42991096191 @default.
- W4299109619 hasRelatedWork W1555590497 @default.
- W4299109619 hasRelatedWork W1966228684 @default.
- W4299109619 hasRelatedWork W2050404753 @default.
- W4299109619 hasRelatedWork W2134900891 @default.
- W4299109619 hasRelatedWork W2135851057 @default.
- W4299109619 hasRelatedWork W2162719345 @default.
- W4299109619 hasRelatedWork W2773418065 @default.
- W4299109619 hasRelatedWork W2791844198 @default.
- W4299109619 hasRelatedWork W2959176998 @default.
- W4299109619 hasRelatedWork W3106547188 @default.
- W4299109619 isParatext "false" @default.
- W4299109619 isRetracted "false" @default.
- W4299109619 workType "article" @default.