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- W4299124615 abstract "SECTION 12 Molecular Diagnostics Book Editor(s):Lynne S. Garcia, Lynne S. Garcia LSG & Associates, Santa Monica, CaliforniaSearch for more papers by this authorHenry D. Isenberg, Henry D. IsenbergSearch for more papers by this author First published: 01 August 2010 https://doi.org/10.1128/9781555817435.ch12 AboutPDFPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShareShare a linkShare onFacebookTwitterLinked InRedditWechat Summary This section deals with molecular techniques that have been used successfully to aid in the detection and identification of pathogens and for the treatment of many infectious diseases. This is followed by an introduction of nucleic acid amplification techniques (NAATs) for which utility in clinical testing was originally complicated by the complexity of the testing processes. These processes include many manual steps, such as nucleic acid extraction, amplification, and detection, and methods were fraught with the potential for false-positive results due to a combination of contamination risks plus the exquisite sensitivity of the testing methods. Some of the commercially available tests that use nucleic acid probes and amplification-based techniques for direct detection of organisms in clinical material are listed in the section. This section provides overall protocols that illustrate the application of molecular techniques used for the management of infectious diseases. It also focuses on molecular methods for epidemiologic typing of microorganisms, and molecular methods for antimicrobial agent resistance determination. The techniques of molecular biology have been used to characterize resistance at the DNA level and may provide rapid, sensitive, and specific information to the clinician for use in therapeutic decision making. Clinical Microbiology Procedures Handbook, Third Edition RelatedInformation" @default.
- W4299124615 created "2022-10-02" @default.
- W4299124615 date "2010-08-01" @default.
- W4299124615 modified "2023-09-23" @default.
- W4299124615 title "Molecular Diagnostics" @default.
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