Matches in SemOpenAlex for { <https://semopenalex.org/work/W4299600243> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W4299600243 abstract "Cellular contractility is essential in diverse aspects of biology, driving processes that range from motility and division, to tissue contraction and mechanical stability, and represents a core element of multi-cellular animal life. In adherent cells, acto-myosin contraction is seen in traction forces that cells exert on their substrate. Dysregulation of cellular contractility appears in a myriad of pathologies, making contractility a promising target in diverse diagnostic approaches using biophysics as a metric. Moreover, novel therapeutic strategies can be based on correcting the apparent malfunction of cell contractility. These applications, however, require direct quantification of these forces. We have developed silicone elastomer-based traction force microscopy (TFM) in a parallelized multi-well format. Our use of a silicone rubber, specifically polydimethylsiloxane (PDMS), rather than the commonly employed hydrogel polyacrylamide (PAA) enables us to make robust and inert substrates with indefinite shelf-lives requiring no specialized storage conditions. Unlike pillar-PDMS based approaches that have a modulus in the GPa range, the PDMS used here is very compliant, ranging from approximately 0.4 kPa to 100 kPa. We create a high-throughput platform for TFM by partitioning these large monolithic substrates spatially into biochemically independent wells, creating a multi-well platform for traction force screening that is compatible with existing multi-well systems. In this manuscript, we use this multi-well traction force system to examine the Epithelial to Mesenchymal Transition (EMT); we induce EMT in NMuMG cells by exposing them to TGF-β, and to quantify the biophysical changes during EMT. We measure the contractility as a function of concentration and duration of TGF-β exposure. Our findings here demonstrate the utility of parallelized TFM in the context of disease biophysics." @default.
- W4299600243 created "2022-10-02" @default.
- W4299600243 creator A5006331400 @default.
- W4299600243 creator A5013305634 @default.
- W4299600243 creator A5013577650 @default.
- W4299600243 creator A5054395481 @default.
- W4299600243 creator A5082063550 @default.
- W4299600243 creator A5083606690 @default.
- W4299600243 date "2019-06-01" @default.
- W4299600243 modified "2023-09-24" @default.
- W4299600243 title "High Throughput Traction Force Microscopy Using PDMS Reveals Dose-Dependent Effects of Transforming Growth Factor-β on the Epithelial-to-Mesenchymal Transition" @default.
- W4299600243 doi "https://doi.org/10.3791/59364-v" @default.
- W4299600243 hasPublicationYear "2019" @default.
- W4299600243 type Work @default.
- W4299600243 citedByCount "0" @default.
- W4299600243 crossrefType "journal-article" @default.
- W4299600243 hasAuthorship W4299600243A5006331400 @default.
- W4299600243 hasAuthorship W4299600243A5013305634 @default.
- W4299600243 hasAuthorship W4299600243A5013577650 @default.
- W4299600243 hasAuthorship W4299600243A5054395481 @default.
- W4299600243 hasAuthorship W4299600243A5082063550 @default.
- W4299600243 hasAuthorship W4299600243A5083606690 @default.
- W4299600243 hasConcept C12554922 @default.
- W4299600243 hasConcept C127413603 @default.
- W4299600243 hasConcept C134018914 @default.
- W4299600243 hasConcept C136229726 @default.
- W4299600243 hasConcept C151730666 @default.
- W4299600243 hasConcept C171250308 @default.
- W4299600243 hasConcept C185592680 @default.
- W4299600243 hasConcept C192562407 @default.
- W4299600243 hasConcept C2779849746 @default.
- W4299600243 hasConcept C2780171871 @default.
- W4299600243 hasConcept C32520587 @default.
- W4299600243 hasConcept C38834483 @default.
- W4299600243 hasConcept C39133596 @default.
- W4299600243 hasConcept C49892992 @default.
- W4299600243 hasConcept C66938386 @default.
- W4299600243 hasConcept C6997183 @default.
- W4299600243 hasConcept C71924100 @default.
- W4299600243 hasConcept C86803240 @default.
- W4299600243 hasConcept C95444343 @default.
- W4299600243 hasConceptScore W4299600243C12554922 @default.
- W4299600243 hasConceptScore W4299600243C127413603 @default.
- W4299600243 hasConceptScore W4299600243C134018914 @default.
- W4299600243 hasConceptScore W4299600243C136229726 @default.
- W4299600243 hasConceptScore W4299600243C151730666 @default.
- W4299600243 hasConceptScore W4299600243C171250308 @default.
- W4299600243 hasConceptScore W4299600243C185592680 @default.
- W4299600243 hasConceptScore W4299600243C192562407 @default.
- W4299600243 hasConceptScore W4299600243C2779849746 @default.
- W4299600243 hasConceptScore W4299600243C2780171871 @default.
- W4299600243 hasConceptScore W4299600243C32520587 @default.
- W4299600243 hasConceptScore W4299600243C38834483 @default.
- W4299600243 hasConceptScore W4299600243C39133596 @default.
- W4299600243 hasConceptScore W4299600243C49892992 @default.
- W4299600243 hasConceptScore W4299600243C66938386 @default.
- W4299600243 hasConceptScore W4299600243C6997183 @default.
- W4299600243 hasConceptScore W4299600243C71924100 @default.
- W4299600243 hasConceptScore W4299600243C86803240 @default.
- W4299600243 hasConceptScore W4299600243C95444343 @default.
- W4299600243 hasIssue "148" @default.
- W4299600243 hasLocation W42996002431 @default.
- W4299600243 hasOpenAccess W4299600243 @default.
- W4299600243 hasPrimaryLocation W42996002431 @default.
- W4299600243 hasRelatedWork W2019663832 @default.
- W4299600243 hasRelatedWork W2087752740 @default.
- W4299600243 hasRelatedWork W2112320300 @default.
- W4299600243 hasRelatedWork W2734723147 @default.
- W4299600243 hasRelatedWork W2743566243 @default.
- W4299600243 hasRelatedWork W2947729025 @default.
- W4299600243 hasRelatedWork W2951502215 @default.
- W4299600243 hasRelatedWork W3082123246 @default.
- W4299600243 hasRelatedWork W4210591574 @default.
- W4299600243 hasRelatedWork W2185565390 @default.
- W4299600243 isParatext "false" @default.
- W4299600243 isRetracted "false" @default.
- W4299600243 workType "article" @default.