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• W4300002796 abstract "Ongoing cell replacement studies and clinical trials have demonstrated the need to control donor and newborn cell behavior within their target tissue. Here we present a methodology to guide stem cell-derived and endogenously regenerated neurons by engineering the microenvironment. Being an “approachable part of the brain,” the eye provides a unique opportunity to study donor neuron fate, migration, and integration within the central nervous system. Glaucoma and other optic neuropathies lead to the permanent loss of retinal ganglion cells (RGCs) – the neurons in the retina that transfer all visual information from the eye to the brain. Cell transplantation and transdifferentiation strategies have been proposed to restore RGCs, and one of the significant barriers to successful RGC integration into the existing retinal circuitry is cell migration towards their natural position in the retina. Here we describe a framework for identifying, selecting, and applying chemokines to direct cell migration in vivo within the retina. We have performed an in silico analysis of the single-cell transcriptome of the developing human retina and identified six receptor-ligand candidates to guide stem cell-derived or newborn neurons. The lead candidates were then tested in functional in vitro assays for their ability to guide stem cell-derived RGCs. For the in vivo studies, donor and newborn neurons were differentiated in human and mouse retinal organoids or endogenously reprogrammed with proneuronal transcription factors, respectively. An exogenous stromal cell-derived factor-1 (SDF1) gradient led to a 2.7-fold increase in donor RGC migration into the ganglion cell layer and a 3.3-fold increase in the displacement of newborn RGCs out of the inner nuclear layer. Furthermore, by altering the migratory profile of donor RGCs toward multipolar migration, overall migration was improved in mature retinal tissues. Together, these results highlight the ability and importance of engineering the tissue microenvironment and the individual cells for research and clinical applications in gene and cell therapies. Graphical Abstract In brief, the “in silico – in vitro – in vivo” funnel holds significant potential for identifying targets to control cellular processes in research and clinical applications. In this report, Soucy et al. describes a framework for identifying, selecting, and applying chemokines to direct retinal ganglion cell migration in vivo within the adult mouse retina." @default.
• W4300002796 created "2022-10-03" @default.
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• W4300002796 date "2022-10-01" @default.
• W4300002796 modified "2023-10-01" @default.
• W4300002796 title "Introduced chemokine gradients guide transplanted and regenerated retinal neurons toward their natural position in the retina" @default.
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• W4300002796 cites W2034429149 @default.
• W4300002796 cites W2036245829 @default.
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• W4300002796 cites W2049956106 @default.
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• W4300002796 cites W2081407876 @default.
• W4300002796 cites W2083452535 @default.
• W4300002796 cites W2093304039 @default.
• W4300002796 cites W2094203217 @default.
• W4300002796 cites W2103656923 @default.
• W4300002796 cites W2104315509 @default.
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• W4300002796 cites W2169536434 @default.
• W4300002796 cites W2176746291 @default.
• W4300002796 cites W2193602046 @default.
• W4300002796 cites W2229551171 @default.
• W4300002796 cites W2313749244 @default.
• W4300002796 cites W2318035006 @default.
• W4300002796 cites W2326860902 @default.
• W4300002796 cites W2332292689 @default.
• W4300002796 cites W2537731554 @default.
• W4300002796 cites W2539197373 @default.
• W4300002796 cites W2549085969 @default.
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• W4300002796 doi "https://doi.org/10.1101/2022.09.29.510158" @default.
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